SETD7 / SET9 antibody

Principal name

SETD7 / SET9 antibody

Alternative names for SETD7 / SET9 antibody

SET7, SET7/9, KMT7, KIAA1717, Histone-lysine N-methyltransferase SETD7, Histone H3-K4 methyltransferase SETD7, H3-K4-HMTase SETD7, SET domain-containing protein 7, Lysine N-methyltransferase 7

SwissProt ID

Q6DHG0 (Danre), Q8VHL1 (Mouse), Q8WTS6 (Human)

Gene ID

80854 (SETD7), 2780854 (Q6MAT6), 3020 (H3F3A)

Available reactivities

Hu (Human), Rt (Rat), Ms (Mouse), Ze (Zebrafish), Can (Canine), Mky (Monkey)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Flow Cytometry (F)

Background of SETD7 / SET9 antibody

Similar to acetylation and phosphorylation, histone methylation at the N-terminal tail has emerged as an important role in regulating chromatin dynamics and gene activity. Histone methylation occurs on arginine and lysine residues and is catalyzed by two families of proteins, the protein arginine methyltransferase family and the SET-domain-containing methyltransferase family. Five members have been identified in the arginine methyltransferase family. About 27 are grouped into the SET-domain family, and another 17 make up the PR domain family that is related to the SET domain family. The retinoblastoma protein-interacting zinc finger gene RIZ 1 is a tumor suppressor gene and a FOUNDING member of the PR domain family. RIZ 1 inactivation is commonly found in many types of human cancers and occurs through loss of mRNA expression, frame shift mutation, chromosomal deletion, and missense mutation. RIZ 1 is also a tumor susceptibility gene in mice. The loss of RIZ 1 mRNA in human cancers was shown to associate with DNA methylation of its promoter CpG island. Methylation of the RIZ 1 promoter strongly correlated with lost or decreased RIZ 1 mRNA expression in breast, liver, colon, and lung cancer cell lines as well as in liver cancer tissues.

General readings

Subramanian,K., (2008) Mol. Cell 30 (3), 336-347

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SETD7 / SET9 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SETD7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503322) Mouse IgG2b OTI2D10 Purified Can, Hu, Mky, Ms, Rt F, ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

SETD7 / SET9 (1-366) antibody

Figure 3. SM6006 SETD7 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Skin at 5 µg/ml followed by biotinylated anti-Mouse IgG secondary antibody, Alkaline Phosphatase-Streptavidin and chromogen. Mouse IgG2b s4E5 Purified Hu E, F, ICC/IF, P, WB
0.1 ml / €290.00
  Acris Antibodies GmbH

SETD7 / SET9 (1-366) antibody

Figure 3. SM6006 SETD7 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Skin at 5 µg/ml followed by biotinylated anti-Mouse IgG secondary antibody, Alkaline Phosphatase-Streptavidin and chromogen. Mouse IgG2b s4E5 Purified Hu E, F, ICC/IF, P, WB
50 µl / €220.00
  Acris Antibodies GmbH

SETD7 / SET9 antibody

Western Blot: SETD7/9 Antibody [TA336441] - Analysis of SETD7 in A) MCF7 and B) HeLa lysate using SETD7 antibody at 1:500. Rabbit Purified Hu, Ms ChIP, P, WB
0.2 ml / €325.00
  OriGene Technologies, Inc.

SETD7 / SET9 antibody

WB was performed on nuclear extracts from HeLa cells (HeLa NE , 20 ug) using the antibody against Set9 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. A molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right. Rabbit IgG Purified Hu
0.1 ml / €325.00
  OriGene Technologies, Inc.

SETD7 / SET9 antibody

WB using the antibody against Set9 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. A molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right. Rabbit IgG Purified Hu
50 µg / €325.00
  OriGene Technologies, Inc.

SETD7 / SET9 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SETD7 (RC219244, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SETD7. Mouse IgG2a OTI5B11 Purified Hu F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SETD7 / SET9 antibody

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SETD7 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human). Mouse IgG1 OTI2E3 Purified Hu, Ms F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SETD7 / SET9 (257-367) antibody

Formalin Fixed, Paraffin-Embedded Human Skin tissue stained with SETD7 Antibody Cat.-No AM32803PU-N at 5 µg/ml after heat-induced antigen retrieval. Mouse IgG2a 5B4 Purified Hu E, ICC/IF, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

SETD7 / SET9 (Center) antibody

Figure 2. AP11177PU-N SET9 antibody staining of Formalin-Fixed and Paraffin-Embedded Human cancer tissue. The primary antibody was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

SETD7 / SET9 (C-term) antibody

Western blot analysis of anti-SET7 Pab(Cat. #AP11178PU-N) in mouse brain tissue lysate (35ug/lane). SET7(arrow) was detected using the purified Pab (1:70 dilution). Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

SETD7 / SET9 antibody

SET7 polyclonal antibody (A01), Lot # 051212JC01 Western Blot analysis of SET7 expression in MCF-7 ( Cat # L046V1 ). Mouse Serum Hu, Rt E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

SETD7 / SET9 antibody

Immunoperoxidase of monoclonal antibody to SETD7 on formalin-fixed paraffin-embedded human colon. [antibody concentration 0.5 ug/ml] Mouse IgG2a 5B4 Purified Hu E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SETD7 / SET9 antibody

Immunoblot analysis of recombinant SETD7 was resolved by electrophoresis , transferred to PVDF membrane and probed with SETD7 monoclonal antibody , clone s4E5 ( 1 : 1000 ) ( Cat # MAB1053 ) . Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and a DAP detection system. Arrow indicated SETD7 ( ~ 50 kDa ) . Mouse IgG2b s4E5 Hu E, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

SETD7 / SET9 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the SETD7 polyclonal antibody ( Cat # PAB2369 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma. Rabbit Hu, Ms E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

SETD7 / SET9 antibody

Western blot analysis of SETD7 polyclonal antibody ( Cat # PAB2370 ) in mouse brain tissue lysate ( 35 µg/lane ) . SETD7 ( arrow ) was detected using the purified polyclonal antibody ( 1 : 70 dilution ) . Rabbit Hu E, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

SETD7 / SET9 antibody

Human Pancreas; WB Suggested Anti-SETD7 Antibody Titration: 5.0ug/ml. Positive Control: Human Pancreas; SETD7 antibody - C-terminal region (ARP48940_T100) in Human Pancreas cells using Western Blot Rabbit Aff - Purified Hu, Ms, Rt, Ze WB
0.1 mg / €310.00
  AVIVA Systems Biology

SETD7 / SET9 antibody

Immunohistochemistry-Paraffin: SETD7 Antibody [NB100-93288] - FFPE section of human prostate carcinoma.  Affinity purified rabbit anti-SET7 used at a dilution of 1:250  Detection: DAB staining using Immunohistochemistry Accessory Kit Rabbit IgG Aff - Purified Hu, Ms IP, P, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

SETD7 / SET9

SETD7 / SET9 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

SETD7 / SET9

SETD7 / SET9 Human Purified > 95 % by SDS-PAGE E. coli
0.1 mg / €230.00
  Acris Antibodies GmbH

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