SETD8 / SET07 antibody

Principal name

SETD8 / SET07 antibody

Alternative names for SETD8 / SET07 antibody

SET8, PRSET7, PR-Set7, KMT5A, Histone-lysine N-methyltransferase SETD8, H4-K20-HMTase SETD8, SET domain-containing protein 8, PR/SET domain-containing protein 07, Lysine N-methyltransferase 5A

SwissProt ID

Q2YDJ8 (Bovin), Q2YDW7 (Mouse), Q9NQR1 (Human), Q9VFK6 (Drome)

Gene ID

387893 (SETD8)

Available reactivities

Hu (Human), Ms (Mouse)

Available hosts

Mouse, Rabbit

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E)

Background of SETD8 / SET07 antibody

SET07 is a histone methyltransferase that methylates Lys-20 of histone H4. H4 Lys-20 methylation represents a specific tag for epigenetic transcriptional repression. The nuclear SET07 protein, which associates with silent chromatin on euchromatic arms but shows no association with constitutive heterochromatin, prefers nucleosomes as substrate compared to free histones. It appears that SET07 may play a role in maintaining silent chromatin by preventing neighboring acetylation of H4 tail. Although the SET domain contains the active site of enzymatic activity, both sequences upstream and downstream of the SET domain are required for methyltransferase activity.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SETD8 / SET07 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SETD8 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808172)(1:150) Mouse IgG1 OTI3G9 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SETD8 / SET07 antibody

Western Blot: SETD8 Antibody - Western blot analysis of SETD8 in 1. overexpression SETD8 vector and 2. empty vector. Rabbit Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SETD8 / SET07 antibody

WB was performed on nuclear extracts from HeLa cells (HeLa NE, 20 ug) using the antibody against SETD8 diluted 1:1000 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right. Rabbit IgG Purified Hu
0.1 ml / €325.00
  OriGene Technologies, Inc.

SETD8 / SET07 antibody

WB was performed on nuclear extracts from HeLa cells (HeLa NE, 20 ug) using the antibody against SETD8 diluted 1:1000 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the right; the location of the protein of interest is indicated by an arrow. Rabbit IgG Purified Hu, Ms
50 µg / €325.00
  OriGene Technologies, Inc.

SETD8 / SET07 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SETD8 (RC208219, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SETD8.(1:2000) Mouse IgG1 OTI5C7 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SETD8 / SET07 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SETD8 (RC208219, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SETD8.(1:2000) Mouse IgG2a OTI2B3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SETD8 / SET07 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SETD8 (RC208219, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SETD8.(1:2000) Mouse IgG1 OTI5E5 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SETD8 / SET07 antibody

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-SETD8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808272)(1:150) Mouse IgG1 OTI2F5 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SETD8 / SET07 (N-term) antibody

Figure 1. Western blot analysis using anti-SET07 Mab (Cat. #AM11037PU-N) to detect SET07 in SAOS-2 (lane 1) and NIH/3T3 cell lysates (lane 2). Mouse IgG1 43AT551.86 Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

SETD8 / SET07 (N-term) antibody

Figure 1. Western blot analysis using anti-SET07 Mab (Cat. #AM11038PU-N) to detect SET07 in 293 cells. Mouse IgG1 43AT890 Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

SETD8 / SET07 (N-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

SETD8 / SET07 (C-term) antibody

Western blot analysis of SET07 Antibody (C-term) Cat.-No AP11171PU-N in K562 cell line lysates (35ug/lane). SET07 (arrow) was detected using the purified Pab. Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

SETD8 / SET07 (Center) antibody

Western blot analysis of SETD8 antibody (Center) (AP17740PU-N) in 293 cell line lysates (35ug/lane). SETD8 (arrow) was detected using the purified Pab. Rabbit Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

SETD8 / SET07 antibody

The SETD8 monoclonal antibody, clone 43AT551.86.76. ( Cat # MAB1153 ) is used in Western blot to detect SETD8 in SAOS-2 ( lane 1 ) and NIH/3T3 cell lysates ( lane 2 ) . Mouse IgG1 43AT551.86.76. Purified Hu, Ms E, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

SETD8 / SET07 antibody

The SETD8 monoclonal antibody, clone 43AT890 ( Cat # MAB1154 ) is used in Western blot to detect SETD8 in 293 cells. Mouse IgG1 43AT890 Purified Hu, Ms E, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

SETD8 / SET07 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the SETD8 polyclonal antibody ( Cat # PAB2363 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma. Rabbit Hu, Ms E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

SETD8 / SET07 antibody

SETD8 / SET07 Rabbit Hu E
0.1 mg / €390.00
  Abnova Taiwan Corp.

SETD8 / SET07 antibody

Western blot was performed on HeLa nuclear extract ( HeLa NE, 20 µg ) using SETD8 polyclonal antibody ( Cat # PAB14114 ) at dilution 1 : 1000 in TBS-Tween + 5% skimmed milk. Rabbit Purified Hu, Ms WB
50 µg / €390.00
  Abnova Taiwan Corp.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

SETD8 / SET07 (full length, N-term HIS tag)

SETD8 / SET07 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

SETD8 overexpression lysate

SETD8 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

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