SETDB1 antibody

Principal name

SETDB1 antibody

Alternative names for SETDB1 antibody

ESET, KMT1E, KIAA0067, Histone-lysine N-methyltransferase SETDB1, SET domain bifurcated 1, ERG-associated protein with SET domain, Histone H3-K9 methyltransferase 4, H3-K9-HMTase 4, Lysine N-methyltransferase 1E

SwissProt ID

O88974 (Mouse), Q15047 (Human), Q32KD2 (Drome), Q6INA9 (Xenla)

Gene ID

9869 (SETDB1)

Available reactivities

Hu (Human), Mky (Monkey), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection))

Background of SETDB1 antibody

The SET domain is a highly conserved,
approximately 150-amino acid motif implicated in the modulation of chromatin structure. It was originally identified as part of a larger
conserved region present in the Drosophila trithorax protein and was subsequently identified in the Drosophila Su(var)3-9 and 'Enhancer of zeste' proteins, from which the acronym SET is
derived. Studies have suggested that the SET domain may be a signature of proteins that modulate transcriptionally active or repressed chromatin states through chromatin remodeling activities. ESET functions as a histone methyltransferase by methylation of Lys-9 of histone H3. H3 Lys-9 methylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. ESET shows a nuclear localization and is associated with non-pericentromeric regions of chromatin, and is excluded from nucleoli and islands of
condensed chromatin. Although ESET is a widely expressed protein, it is highly expressed in the testis.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SETDB1 antibody

  Rabbit polyclonal anti-ESET antibody  
WB analysis using Anti-ESET antibody to detect human ESET present in a 293 whole cell lysate.   ~30µg of lysate was loaded per lane for SDS-PAGE.  Comparison to a molecular weight marker (not shown) indicates a single band of ~170 kDa is detected.  Peptide competition (not shown) blocks staining of this band.  The blot was incubated with a 1:1000 dilution of the antibody at RT for 2 h followed by detection using IRDye™800 labeled Goat-a-Rabbit IgG [H&L]  diluted 1:5,000 for 45 min. Rabbit Aff - Purified Hu E, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

SETDB1 antibody

  Rabbit anti-SETDB1 Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using SETDB1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

SETDB1 antibody

Western blot analysis using SETDB1 antibody Cat.-No AM06545SU-N against MCF-7 (1), T47D (2), HEK293 (3), JURKAT (4), NIH/3T3 (5) and F9 (6) cell lysate. Mouse IgG1 5H6D4 Ascites Hu, Mky, Ms E, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

SETDB1 antibody

Immunofluorescence analysis of LOVO cells using SETDB1 antibody Cat.-No AM06546SU-N (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Mouse IgG1 5H6A12 Ascites Hu, Mky, Ms E, ICC/IF, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

SETDB1 antibody

Figure 1. Staining SETDB1 in Testis by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue. Rabbit Aff - Purified Hu E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

SETDB1 (C-term) antibody

LEFT: Western blot analysis of anti-SETDB1 Pab(Cat. #AP11091PU-N) in Ramos cell line lysate (35ug/lane). SETDB1 (arrow) was detected using the purified Pab (1:60 dilution). RIGHT: Western blot analysis of SETDB1(arrow) using rabbit polyclonal SETDB1 Antibody (C-term) (Cat.#AP11091PU-N). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SETDB1 gene (Lane 2) (Origene Technologies). Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

SETDB1 (transcript variant 2)

  Purified recombinant protein of Homo sapiens SET domain, bifurcated 1 (SETDB1), transcript variant 2  
SETDB1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

SETDB1 (transcript variant 1)

  Recombinant protein of human SET domain, bifurcated 1 (SETDB1), transcript variant 1  
SETDB1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

SETDB1 overexpression lysate

SETDB1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

SETDB1 overexpression lysate

SETDB1 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, SETDB1 (mGFP-tagged) - Human SET domain, bifurcated 1 (SETDB1), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, SETDB1 (mGFP-tagged) - Human SET domain, bifurcated 1 (SETDB1), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €1,320.00
  OriGene Technologies, Inc.

Lenti ORF particles, SETDB1 (Myc-DDK tagged) - Human SET domain, bifurcated 1 (SETDB1), transcript variant 2 , 200 uL, >10^7 TU/mL

Lenti ORF particles, SETDB1 (Myc-DDK tagged) - Human SET domain, bifurcated 1 (SETDB1), transcript variant 2 , 200 uL, >10^7 TU/mL  
200 µl / €1,180.00
  OriGene Technologies, Inc.

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