SETDB1 antibody

Principal name

SETDB1 antibody

Alternative names for SETDB1 antibody

ESET, KMT1E, KIAA0067, Histone-lysine N-methyltransferase SETDB1, SET domain bifurcated 1, ERG-associated protein with SET domain, Histone H3-K9 methyltransferase 4, H3-K9-HMTase 4, Lysine N-methyltransferase 1E

SwissProt ID

O88974 (Mouse), Q15047 (Human), Q32KD2 (Drome), Q6INA9 (Xenla)

Gene ID

9869 (SETDB1), 986992 (accA)

Available reactivities

Hu (Human), Ms (Mouse), Mky (Monkey), Rt (Rat)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ChIP assay (ChIP), Immunoprecipitation (IP), Paraffin Sections (P), PLA/Proximity ligation assay (PLA), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection))

Background of SETDB1 antibody

The SET domain is a highly conserved,
approximately 150-amino acid motif implicated in the modulation of chromatin structure. It was originally identified as part of a larger
conserved region present in the Drosophila trithorax protein and was subsequently identified in the Drosophila Su(var)3-9 and 'Enhancer of zeste' proteins, from which the acronym SET is
derived. Studies have suggested that the SET domain may be a signature of proteins that modulate transcriptionally active or repressed chromatin states through chromatin remodeling activities. ESET functions as a histone methyltransferase by methylation of Lys-9 of histone H3. H3 Lys-9 methylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. ESET shows a nuclear localization and is associated with non-pericentromeric regions of chromatin, and is excluded from nucleoli and islands of
condensed chromatin. Although ESET is a widely expressed protein, it is highly expressed in the testis.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SETDB1 antibody

WB analysis using Anti-ESET antibody to detect human ESET present in a 293 whole cell lysate.   ~30µg of lysate was loaded per lane for SDS-PAGE.  Comparison to a molecular weight marker (not shown) indicates a single band of ~170 kDa is detected.  Peptide competition (not shown) blocks staining of this band.  The blot was incubated with a 1:1000 dilution of the antibody at RT for 2 h followed by detection using IRDye™800 labeled Goat-a-Rabbit IgG [H&L]  diluted 1:5,000 for 45 min. Rabbit Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SETDB1 antibody

Western blot analysis of extracts of various cell lines, using SETDB1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

SETDB1 antibody

Western blot analysis using SETDB1 antibody Cat.-No AM06545SU-N against MCF-7 (1), T47D (2), HEK293 (3), JURKAT (4), NIH/3T3 (5) and F9 (6) cell lysate. Mouse IgG1 5H6D4 Ascites Hu, Mky, Ms E, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

SETDB1 antibody

Immunofluorescence analysis of LOVO cells using SETDB1 antibody Cat.-No AM06546SU-N (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Mouse IgG1 5H6A12 Ascites Hu, Mky, Ms E, ICC/IF, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

SETDB1 antibody

Figure 1. Staining SETDB1 in Testis by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue. Rabbit Aff - Purified Hu E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

SETDB1 (C-term) antibody

LEFT: Western blot analysis of anti-SETDB1 Pab(Cat. #AP11091PU-N) in Ramos cell line lysate (35ug/lane). SETDB1 (arrow) was detected using the purified Pab (1:60 dilution). RIGHT: Western blot analysis of SETDB1(arrow) using rabbit polyclonal SETDB1 Antibody (C-term) (Cat.#AP11091PU-N). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SETDB1 gene (Lane 2) (Origene Technologies). Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

SETDB1 antibody

SETDB1 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

SETDB1 antibody

Western Blot analysis of SETDB1 expression in transfected 293T cell line (H00009869-T01) by SETDB1 MaxPab polyclonal antibody.

Lane 1: SETDB1 transfected lysate(141.9 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

SETDB1 antibody

Immunofluorescence of monoclonal antibody to SETDB1 on HeLa cell. [antibody concentration 10 ug/ml] Mouse IgG2a 4A3 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SETDB1 antibody

SETDB1 Mouse IgG2a 4E8 Ascites Hu E, WB
0.2 ml / €370.00
  Abnova Taiwan Corp.

SETDB1 antibody

Western blot analysis is shown using SETDB1 polyclonal antibody ( Cat # PAB10044 ) to detect human SETBD1 present in a 293 whole cell lysate.
Approximately 30 µg of lysate was loaded per lane for SDS-PAGE.
Comparison to a molecular weight marker ( not shown ) indicates a singleband of ~170 kDa is detected.
Peptide competition ( not shown ) blocks staining of this band.
The blot was incubated with a 1:1000 dilution of the antibody at room temperature for 2h followed by detection using IRDyeTM800 labeled Goat-a-Rabbit IgG [H&L] diluted1:5,000 for 45 min.
IRDyeTM800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
IRDye is a trademark of LI-COR, Inc. Hu
0.1 mg / €560.00
  Abnova Taiwan Corp.

SETDB1 antibody

SETDB1 Rabbit IgG Aff - Purified Hu, Ms WB
50 µg / €680.00
  Funakoshi Co., Ltd.

SETDB1 antibody

Western Blot and  Immunoprecipitation: SETDB1 Antibody [NB100-79776] - Detection of Human SETDB1 on HeLa whole cell lysate using NB100-79776. Rabbit anti-SETDB1 antibodies BL538 and  NB100-79776 were used at 1 mcg/mg lysate for IP (B). Rabbit IgG Aff - Purified Hu IP, PLA, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

SETDB1 antibody

Western Blot: SETDB1 Antibody (5H6D4) [NBP1-51676] - Western blot analysis using SETDB1 mouse mAb against MCF-7 (1),T47D (2), HEK293 (3), JURKAT (4), NIH/3T3 (5) and F9 (6) cell lysate. Mouse IgG1 5H6D4 Purified Hu, Mky, Ms E, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

SETDB1 antibody

Immunofluorescence: SETDB1 Antibody (5H6A12) [NBP1-51677] - Immunofluorescence analysis of LOVO cells using SETDB1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Mouse IgG1 5H6A12 Purified Hu, Mky, Ms E, ICC/IF, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

SETDB1 antibody

Immunohistochemistry-Paraffin: SETDB1 Antibody [NBP1-84790] - Immunohistochemical staining of human colon shows strong nuclear, cytoplasmic and membranous positivity in glandular cells. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

SETDB1 (transcript variant 2)

SETDB1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

SETDB1 (transcript variant 1)

SETDB1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

SETDB1

SETDB1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

SETDB1

SETDB1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

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