SF3A1 / SAP114 antibody

Principal name

SF3A1 / SAP114 antibody

Alternative names for SF3A1 / SAP114 antibody

Splicing factor 3A subunit 1, SF3a120, SAP-114, SAP 114, Spliceosome-associated protein 114

SwissProt ID

A2VDN6 (Bovin), Q15459 (Human), Q8K4Z5 (Mouse), Q8RXF1 (Arath)

Gene ID

10291 (SF3A1), 67465 (Sf3a1)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Dros (Drosophila), Ms (Mouse), Rt (Rat), Ze (Zebrafish), African clawed frog, Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of SF3A1 / SAP114 antibody

SF3A1 is the subunit 1 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 1 belongs to the SURP protein family; named for the SURP motifs that are thought to mediate RNA binding. Subunit 1 has tandemly repeated SURP motifs in its amino-terminal half while its carboxy-terminal half contains a proline-rich region and a ubiquitin-like domain. Binding studies with truncated subunit 1 derivatives demonstrated that the two SURP motifs are necessary for binding to subunit 3 while contacts with subunit 2 may occur through sequences carboxy-terminal to the SURP motifs.This gene encodes subunit 1 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 1 belongs to the SURP protein family; named for the SURP (also called SWAP or Suppressor-of-White-APricot) motifs that are thought to mediate RNA binding. Subunit 1 has tandemly repeated SURP motifs in its amino-terminal half while its carboxy-terminal half contains a proline-rich region and a ubiquitin-like domain. Binding studies with truncated subunit 1 derivatives demonstrated that the two SURP motifs are necessary for binding to subunit 3 while contacts with subunit 2 may occur through sequences carboxy-terminal to the SURP motifs. Alternative splicing results in multiple transcript variants encoding different isoforms.

General readings

Ewing,R.M., Mol. Syst. Biol. 3, 89 (2007)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SF3A1 / SAP114 antibody

  SF3A1 mouse monoclonal antibody, clone OTI2C4 (formerly 2C4)  
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SF3A1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800587) Mouse IgG1 OTI2C4 Purified Hu P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

SF3A1 / SAP114 antibody

  SF3A1 mouse monoclonal antibody, clone OTI4D8 (formerly 4D8)  
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-SF3A1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800588) Mouse IgG1 OTI4D8 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SF3A1 / SAP114 antibody

  SF3A1 mouse monoclonal antibody, clone OTI3F4 (formerly 3F4)  
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-SF3A1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800622) Mouse IgG2a OTI3F4 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SF3A1 / SAP114 antibody

  Rabbit Polyclonal Anti-SF3A1 Antibody  
WB Suggested Anti-SF3A1 Antibody Titration: 0.2-1 ug/ml; Positive Control: 721_B cell lysate; SF3A1 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze ICC/IF, WB
50 µg / €325.00
  OriGene Technologies, Inc.

SF3A1 / SAP114 antibody

  Rabbit Polyclonal Anti-SF3A1 Antibody  
Host: Rabbit; Target Name: SF3A1; Sample Tissue: Human Adult Placenta; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

SF3A1 / SAP114 antibody

  SF3A1 mouse monoclonal antibody, clone OTI8H1 (formerly 8H1)  
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-SF3A1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800461) Mouse IgG2b OTI8H1 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SF3A1 / SAP114 antibody

  SF3A1 mouse monoclonal antibody, clone OTI1F10 (formerly 1F10)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SF3A1 (RC201098, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SF3A1. Mouse IgG1 OTI1F10 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SF3A1 / SAP114 antibody

  SF3A1 mouse monoclonal antibody, clone OTI1B5 (formerly 1B5)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SF3A1 (RC201098, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SF3A1. Mouse IgG1 OTI1B5 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SF3A1 / SAP114 antibody

  SF3A1 mouse monoclonal antibody, clone OTI7G6 (formerly 7G6)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SF3A1 (RC201098, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SF3A1. Mouse IgG2a OTI7G6 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CRISPR

Catalog No. Species Donor Vector  

SF3A1 - human gene knockout kit via CRISPR

SF3A1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

SF3A1 - human gene knockout kit via CRISPR

SF3A1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

SF3A1 - human gene knockout kit via CRISPR

SF3A1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

SF3A1 - human gene knockout kit via CRISPR

SF3A1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Sf3a1 - mouse gene knockout kit via CRISPR

Sf3a1 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Sf3a1 - mouse gene knockout kit via CRISPR

Sf3a1 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Sf3a1 - mouse gene knockout kit via CRISPR

Sf3a1 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Sf3a1 - mouse gene knockout kit via CRISPR

Sf3a1 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

SF3A1 / SAP114 (transcript variant 1)

  Recombinant protein of human splicing factor 3a, subunit 1, 120kDa (SF3A1), transcript variant 1  
SF3A1 / SAP114 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, SF3A1 (mGFP-tagged) - Human splicing factor 3a, subunit 1, 120kDa (SF3A1), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, SF3A1 (mGFP-tagged) - Human splicing factor 3a, subunit 1, 120kDa (SF3A1), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, SF3A1 (Myc-DDK tagged) - Human splicing factor 3a, subunit 1, 120kDa (SF3A1), transcript variant 1 , 200 uL, >10^7 TU/mL

Lenti ORF particles, SF3A1 (Myc-DDK tagged) - Human splicing factor 3a, subunit 1, 120kDa (SF3A1), transcript variant 1 , 200 uL, >10^7 TU/mL  
200 µl / €940.00
  OriGene Technologies, Inc.

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