SF3B1 / SAP155 antibody

Principal name

SF3B1 / SAP155 antibody

Alternative names for SF3B1 / SAP155 antibody

Splicing factor 3B subunit 1, SAP-155, SAP 155, SF3b155, Pre-mRNA-splicing factor SF3b 155 kDa subunit, Spliceosome-associated protein 155

SwissProt ID

O57683 (Xenla), O75533 (Human), P49955 (Yeast), Q10178 (Schpo), Q99NB9 (Mouse)

Gene ID

23451 (SF3B1)

Available reactivities

Can (Canine), Chk (Chicken), Hu (Human), Ms (Mouse), Rt (Rat), Ze (Zebrafish), African clawed frog, Dros (Drosophila), Hst (Hamster), Bov (Bovine), Eq (Equine), GP (Guinea Pig), Gt (Goat), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C)

Background of SF3B1 / SAP155 antibody

SF3B1 is subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron\'s branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures.This gene encodes subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron\'s branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures. Alternative splicing results in multiple transcript variants encoding different isoforms.

General readings

Beausoleil,S.A., (2004) Proc. Natl. Acad. Sci. U.S.A. 101 (33), 12130-12135

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SF3B1 / SAP155 antibody

WB Suggested Anti-SF3B1 Antibody Titration: 1.25 ug/ml; Positive Control: Human Thymus Rabbit IgG Purified Bov, Can, Eq, GP, Gt, Hu, Ms, Por, Rb, Rt, Ze P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SF3B1 / SAP155 antibody

Human Lung; . Rabbit Anti-SF3B1 Antibody. . Catalog Number:. ARP40249 . Paraffin Embedded Tissue: Human alveolar cell . Cellular Data: Epithelial cells of renal tubule. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X Rabbit Aff - Purified African clawed frog, Can, Chk, Dros, Hu, Ms, Rt, Ze P, WB
0.1 mg / €310.00
  AVIVA Systems Biology

SF3B1 / SAP155 antibody

WB Suggested Anti-SF3B1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Hela cell lysateSF3B1 is strongly supported by BioGPS gene expression data to be expressed in Human HeLa cells Rabbit IgG Purified Bov, Can, Eq, GP, Gt, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

SF3B1 / SAP155 antibody

SF3B1 / SAP155 Rabbit IgG Purified Bov, Can, Eq, GP, Gt, Hu, Ms, Por, Rt, Ze WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SF3B1 / SAP155 antibody

WB Suggested Anti-SF3B1 Antibody Titration: 1.25 ug/ml; ELISA Titer: 1: 62500; Positive Control: Jurkat cell lysate; SF3B1 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells Rabbit IgG Purified Bov, Can, Eq, GP, Gt, Hu, Ms, Por, Rb, Rt, Ze P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SF3B1 / SAP155 antibody

Western blot analysis of Sap155 expression in several cells using AM26484AF-N. Mouse IgG2b 1A5 Azide Free Hst, Hu, Ms WB
0.1 mg / €430.00
  Acris Antibodies GmbH

SF3B1 / SAP155 (98-198) antibody

Immunocytochemical detection of Sap155 on 4% PFA fixed HeLa cells with AM26528AF-N. Mouse IgG2b 16 Azide Free Hu, Ms ICC/IF, IP, WB
0.1 mg / €390.00
  Acris Antibodies GmbH

SF3B1 / SAP155 antibody

Human Heart; . Rabbit Anti-SF3B1 Antibody. . Catalog Number:. ARP40248 . Paraffin Embedded Tissue: Human cardiac cell . Cellular Data: Epithelial cells of renal tubule. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X Rabbit IgG Purified African clawed frog, Can, Chk, Dros, Hu, Ms, Rt, Ze P, WB
0.1 mg / €310.00
  AVIVA Systems Biology

SF3B1 / SAP155 antibody

Human HeLa; WB Suggested Anti-SF3B1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Hela cell lysate. SF3B1 is strongly supported by BioGPS gene expression data to be expressed in Human HeLa cells.; SF3B1 antibody - N-terminal region (ARP40732_P050) in Human HeLa cells using Western Blot Rabbit Aff - Purified African clawed frog, Chk, Dros, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

SF3B1 / SAP155 antibody

Human Jurkat; WB Suggested Anti-SF3B1 Antibody Titration: 0.3125ug/ml. ELISA Titer: 1:312500. Positive Control: Jurkat cell lysate. SF3B1 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells.; SF3B1 antibody - N-terminal region (ARP40733_T100) in Human Jurkat cells using Western Blot Rabbit IgG Purified African clawed frog, Can, Chk, Hu, Ms, Rt, Ze WB
0.1 mg / €310.00
  AVIVA Systems Biology

SF3B1 / SAP155 antibody

Western blot analysis of SAP155 expression in A431 (A); Ramos (B); SP2/0 (C); H9C2 (D) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

SF3B1 / SAP155 antibody

Western blot analysis of SAP155 expression in A431 (A); Ramos (B); SP2/0 (C); H9C2 (D) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

SF3B1 / SAP155 antibody

Western blot analysis of SAP155 expression in A431 (A); Ramos (B); SP2/0 (C); H9C2 (D) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

SF3B1 / SAP155 antibody

Western Blot and IP: SAP155 Antibody [NB100-55255] - Detection of Human SF3b155/SAP155 on HeLa whole cell lysate using NB100-55255. SF3b155/SAP155 was also immunoprecipitated by rabbit anti-SF3b155/SAP155 antibody NB100-55256. Rabbit Aff - Purified Hu IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

SF3B1 / SAP155 antibody

Immunohistochemistry-Paraffin: SAP155 Antibody [NB100-55256] - See Notes, IHC Caption B Rabbit Aff - Purified Hu IP, P, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

SF3B1 / SAP155 antibody

SF3B1 / SAP155 Rabbit Purified Can, Hu, Ms, Rt P, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

SF3B1 / SAP155 (transcript variant 2)

SF3B1 / SAP155 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

SF3B1 overexpression lysate

SF3B1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

18 Item(s)

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