SF3B14 / SAP14 antibody

Principal name

SF3B14 / SAP14 antibody

Alternative names for SF3B14 / SAP14 antibody

Splicing factor 3B 14 kDa subunit, CGI-110, HSPC175, HT006, SAP-14, SAP 14, SF3B14a, Pre-mRNA branch site protein p14

SwissProt ID

P59708 (Mouse), Q8ITY4 (Caeel), Q9FMP4 (Arath), Q9VRV7 (Drome), Q9Y3B4 (Human)

Gene ID

51639 (SF3B14)

Available reactivities

Bov (Bovine), Can (Canine), Chk (Chicken), Hu (Human), Ms (Mouse), Ze (Zebrafish), African clawed frog, Rt (Rat), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of SF3B14 / SAP14 antibody

SF3B14 is a 14 kDa protein subunit of the splicing factor 3b complex. Splicing factor 3b associates with both the U2 and U11/U12 small nuclear ribonucleoprotein complexes (U2 snRNP) of spliceosomes. This 14 kDa protein interacts directly with subunit 1 of the splicing factor 3b complex. SF3B14 also interacts directly with the adenosine that carries out the first transesterification step of splicing at the pre-mRNA branch site. This gene encodes a 14 kDa protein subunit of the splicing factor 3b complex. Splicing factor 3b associates with both the U2 and U11/U12 small nuclear ribonucleoprotein complexes (U2 snRNP) of spliceosomes. This 14 kDa protein interacts directly with subunit 1 of the splicing factor 3b complex. This 14 kDa protein also interacts directly with the adenosine that carries out the first transesterification step of splicing at the pre-mRNA branch site.

General readings

Golas MM., et al. (2003) Science. 300(5621):980-4.
Will CL., et al. (2002) EMBO J. 21(18):4978-88.

20 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SF3B14 / SAP14 antibody

Western blot analysis of extracts from HepG2 cells, using SF3B14 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SF3B14 / SAP14 antibody

WB Suggested Anti-SF3B14 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: THP-1 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

SF3B14 / SAP14 antibody

WB Suggested Anti-SF3B14 Antibody Titration: 0.2-1 ug/ml; Positive Control: Human Placenta Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

SF3B14 / SAP14 antibody

Western blot analysis of extracts from HepG2 cells, using SF3B14 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, WB
50 µg / €205.00
  Assay Biotech

SF3B14 / SAP14 antibody

Western blot analysis of extracts from HepG2 cells, using SF3B14 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, WB
0.1 mg / €310.00
  Assay Biotech

SF3B14 / SAP14 antibody

Human THP-1; WB Suggested Anti-SF3B14 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: THP-1 cell lysate; SF3B14 antibody - N-terminal region (ARP40828_P050) in Human THP-1 cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

SF3B14 / SAP14 antibody

Human Placenta; WB Suggested Anti-SF3B14 Antibody Titration: 0.2-1 ug/ml. Positive Control: Human Placenta; SF3B14 antibody - middle region (ARP40829_P050) in Human Placenta cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

SF3B14 / SAP14 antibody

Immunofluorescent analysis of SAP14 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4  C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Bov, Hu, Ms, Rt, Ze ICC/IF, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

SF3B14 / SAP14 antibody

Immunofluorescent analysis of SAP14 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4  C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Bov, Hu, Ms, Rt, Ze ICC/IF, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

SF3B14 / SAP14 antibody

Immunofluorescent analysis of SAP14 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4  C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Bov, Hu, Ms, Rt, Ze ICC/IF, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

SF3B14 / SAP14 antibody

Western Blot: SF3B14 Antibody [NBP1-57226] - This Anti-SF3B14 antibody was used in Western Blot of THP-1     tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu, Ms WB
50 µg / €440.00
  Novus Biologicals Inc.

SF3B14 / SAP14 antibody

Immunohistochemistry-Paraffin: SF3B14 Antibody [NBP1-87430] - Immunohistochemical staining of human placenta shows strong nuclear positivity in decidual cells. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

SF3B14 / SAP14 antibody

Immunohistochemistry-Paraffin: SF3B14 Antibody [NBP1-87431] - Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

SF3B14 / SAP14 (full length, N-term HIS tag)

SF3B14 / SAP14 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

SF3B14 / SAP14 (1-125, His-tag)

SF3B14 / SAP14 Human Purified > 95 % E. coli
0.25 mg / €750.00
  Acris Antibodies GmbH

SF3B14 / SAP14 (1-125, His-tag)

SF3B14 / SAP14 Human Purified > 95 % E. coli
50 µg / €300.00
  Acris Antibodies GmbH

SF3B14 / SAP14

SF3B14 / SAP14 Human in vitro transl.
2 µg / €300.00
  Abnova Taiwan Corp.

SF3B14 / SAP14

SF3B14 / SAP14 Human Purified
0.25 mg / €870.00
  Novus Biologicals Inc.

SF3B14 / SAP14

SF3B14 / SAP14 Human Purified
50 µg / €380.00
  Novus Biologicals Inc.

Lysates

Catalog No.    

SF3B6 overexpression lysate

SF3B6 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

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