SF3B3 / SAP130 antibody

Principal name

SF3B3 / SAP130 antibody

Alternative names for SF3B3 / SAP130 antibody

Splicing factor 3B subunit 3, SF3b130, SAP-130, SAP 130, STAF130, KIAA0017, Spliceosome-associated protein 130, Pre-mRNA-splicing factor SF3b 130 kDa subunit

SwissProt ID

A0JN52 (Bovin), Q15393 (Human), Q1LVE8 (Danre), Q921M3 (Mouse)

Gene ID

23450 (SF3B3), 101943 (Sf3b3)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Rt (Rat), Ze (Zebrafish), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Goat, Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of SF3B3 / SAP130 antibody

SF3B3 is subunit 3 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron\'s branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 3 has also been identified as a component of the STAGA (SPT3-TAF(II)31-GCN5L acetylase) transcription coactivator-HAT (histone acetyltransferase) complex, and the TFTC (TATA-binding-protein-free TAF(II)-containing complex). These complexes may function in chromatin modification, transcription, splicing, and DNA repair.This gene encodes subunit 3 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron\'s branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 3 has also been identified as a component of the STAGA (SPT3-TAF(II)31-GCN5L acetylase) transcription coactivator-HAT (histone acetyltransferase) complex, and the TFTC (TATA-binding-protein-free TAF(II)-containing complex). These complexes may function in chromatin modification, transcription, splicing, and DNA repair. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Das BK, Xia L, Palandjian L, Gozani O, Chyung Y, Reed R.
Characterization of a protein complex containing spliceosomal proteins SAPs 49, 130, 145, and 155.
Mol Cell Biol 1999 Oct;19(10):6796-802
PMID: 10490618

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SF3B3 / SAP130 (C-term) antibody

  Goat Polyclonal Antibody against SAP130 / SF3B3  
TA302598 staining (0.1µg/ml) of nuclear lysate from HeLa cells (RIPA buffer, 30µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SF3B3 / SAP130 antibody

  Rabbit polyclonal antibody to SAP130 (splicing factor 3b, subunit 3, 130kDa)  
SAP130 antibody [C3], C-term detects SAP130 protein by western blot analysis. A. 30 ug HeLa whole cell extract. 5 % SDS-PAGE. SAP130 antibody [C3], C-term (TA308399) dilution: 1:10000 Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SF3B3 / SAP130 antibody

  Rabbit Polyclonal Anti-SF3B3 Antibody  
WB Suggested Anti-SF3B3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: HCT15 cell lysate; SF3B3 is supported by BioGPS gene expression data to be expressed in HCT15 Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

SF3B3 / SAP130 antibody

  Rabbit Polyclonal Anti-SF3B3 Antibody  
WB Suggested Anti-SF3B3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Transfected 293T Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

SF3B3 / SAP130 (C-term) antibody

Western blot analysis using SF3B3 Antibody (C-term) Cat.-No AP53870PU-N in CEM cell line lysates (35ug/lane). This demonstrates the SF3B3 antibody detected the SF3B3 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

SF3B3 - human gene knockout kit via CRISPR

SF3B3 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

SF3B3 - human gene knockout kit via CRISPR

SF3B3 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

SF3B3 - human gene knockout kit via CRISPR

SF3B3 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

SF3B3 - human gene knockout kit via CRISPR

SF3B3 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Sf3b3 - mouse gene knockout kit via CRISPR

Sf3b3 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Sf3b3 - mouse gene knockout kit via CRISPR

Sf3b3 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Sf3b3 - mouse gene knockout kit via CRISPR

Sf3b3 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Sf3b3 - mouse gene knockout kit via CRISPR

Sf3b3 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

SF3B3 / SAP130

  Recombinant protein of human splicing factor 3b, subunit 3, 130kDa (SF3B3)  
SF3B3 / SAP130 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

14 Item(s)

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