SF3B4 / SAP49 antibody

Principal name

SF3B4 / SAP49 antibody

Alternative names for SF3B4 / SAP49 antibody

Splicing factor 3B subunit 4, SAP-49, SAP 49, SF3b50, Spliceosome-associated protein 49, Pre-mRNA-splicing factor SF3b 49 kDa subunit

SwissProt ID

Q15427 (Human), Q6AYL5 (Rat), Q8QZY9 (Mouse)

Gene ID

10262 (SF3B4)

Available reactivities

Bov (Bovine), Can (Canine), Hu (Human), Ms (Mouse), Rt (Rat), Ze (Zebrafish), African clawed frog, Por (Porcine), Eq (Equine), GP (Guinea Pig), Gt (Goat), Rb (Rabbit), Ye (Yeast)

Available hosts

Rabbit, Goat, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of SF3B4 / SAP49 antibody

SF3B4 is one of four subunits of the splicing factor 3B. The protein cross-links to a region in the pre-mRNA immediately upstream of the branchpoint sequence in pre-mRNA in the prespliceosomal complex A. It also may be involved in the assembly of the B, C and E spliceosomal complexes. In addition to RNA-binding activity, this protein interacts directly and highly specifically with subunit 2 of the splicing factor 3B. This protein contains two N-terminal RNA-recognition motifs (RRMs), consistent with the observation that it binds directly to pre-mRNA.This gene encodes one of four subunits of the splicing factor 3B. The protein encoded by this gene cross-links to a region in the pre-mRNA immediately upstream of the branchpoint sequence in pre-mRNA in the prespliceosomal complex A. It also may be involved in the assembly of the B, C and E spliceosomal complexes. In addition to RNA-binding activity, this protein interacts directly and highly specifically with subunit 2 of the splicing factor 3B. This protein contains two N-terminal RNA-recognition motifs (RRMs), consistent with the observation that it binds directly to pre-mRNA.This gene encodes one of four subunits of the splicing factor 3B. The protein encoded by this gene cross-links to a region in the pre-mRNA immediately upstream of the branchpoint sequence in pre-mRNA in the prespliceosomal complex A. It also may be involved in the assembly of the B, C and E spliceosomal complexes. In addition to RNA-binding activity, this protein interacts directly and highly specifically with subunit 2 of the splicing factor 3B. This protein contains two N-terminal RNA-recognition motifs (RRMs), consistent with the observation that it binds directly to pre-mRNA.

General readings

Champion-Arnaud P, Reed R.
The prespliceosome components SAP 49 and SAP 145 interact in a complex implicated in tethering U2 snRNP to the branch site.
Genes Dev 1994 Aug 15;8(16):1974-83
PMID: 7958871

15 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SF3B4 / SAP49 (N-term) antibody

TA302812 staining (0.1µg/ml) of Hela lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SF3B4 / SAP49 antibody

Western Blot: SF3B4 Antibody (3A1) - Western blot of HeLa cell crude extract stained with anti-SF3B4.  The SF3B4 (3A1) antibody runs with an apparent SDS-PAGE molecular weight 49kDa. Mouse IgG2b 3A1 Purified Bov, Hu, Ms, Por, Rt ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SF3B4 / SAP49 antibody

Western blot analysis of extracts from K562 and Jurkat cells, using SF3B4 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SF3B4 / SAP49 antibody

WB Suggested Anti-SF3B4 Antibody Titration: 1.25 ug/ml; Positive Control: HepG2 cell lysate; SF3B4 is supported by BioGPS gene expression data to be expressed in HepG2 Rabbit IgG Purified Bov, Can, Eq, GP, Gt, Hu, Ms, Por, Rb, Rt, Ye, Ze P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SF3B4 / SAP49 antibody

WB Suggested Anti-SF3B4 Antibody Titration: 0.2-1 ug/ml; Positive Control: Jurkat cell lysate; SF3B4 is supported by BioGPS gene expression data to be expressed in Jurkat Rabbit IgG Purified Bov, Can, Eq, GP, Gt, Hu, Ms, Por, Rb, Rt, Ye, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

SF3B4 / SAP49 antibody

Western Blot analysis of SF3B4 expression in transfected 293T cell line by SF3B4 monoclonal antibody (M03), clone 1B8.

Lane 1: SF3B4 transfected lysate(44.4 KDa).
Lane 2: Non-transfected lysate. Mouse IgG2a 1B8 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SF3B4 / SAP49 antibody

SF3B4 polyclonal antibody ( Cat # PAB6214 ) staining ( 0.1 µg/ml ) of HeLa lysate ( RIPA buffer, 35 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

SF3B4 / SAP49 antibody

Human Intestine; Rabbit Anti-SF3B4 Antibody. Catalog Number: ARP40609. Paraffin Embedded Tissue: Human Intestine. Cellular Data: Epithelial cells of intestinal villas. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X; SF3B4 antibody - N-terminal region (ARP40609_T100) in Human Intestine cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Bov, Can, Hu, Ms, Rt, Ze P, WB
0.1 mg / €310.00
  AVIVA Systems Biology

SF3B4 / SAP49 antibody

Human Jurkat; WB Suggested Anti-SF3B4 Antibody Titration: 0.2-1 ug/ml. Positive Control: Jurkat cell lysate. SF3B4 is supported by BioGPS gene expression data to be expressed in Jurkat.; SF3B4 antibody - N-terminal region (ARP40610_P050) in Human Jurkat cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

SF3B4 / SAP49 antibody

NB100-1076 staining (0.1ug/ml) of Hela lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat IgG Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

SF3B4 / SAP49 antibody

Western Blot: SF3B4 Antibody [NBP1-57419] - This Anti-SF3B4 antibody was used in Western Blot of HepG2 tissue lysate at a concentration of 1.25ug/ml. Rabbit Purified Can, Hu, Ms, Rt, Ze P, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

SF3B4 / SAP49

SF3B4 / SAP49 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

SF3B4 / SAP49

SF3B4 / SAP49 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

SF3B4 / SAP49

SF3B4 / SAP49 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

SF3B4 / SAP49

SF3B4 / SAP49 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

15 Item(s)

per page
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