SFPQ / PSF antibody

Principal name

SFPQ / PSF antibody

Alternative names for SFPQ / PSF antibody

POMP100, Splicing factor proline- and glutamine-rich, Polypyrimidine tract-binding protein-associated-splicing factor, PTB-associated-splicing factor, DNA-binding p52/p100 complex 100 kDa subunit, 100 kDa DNA-pairing protein

SwissProt ID

P23246 (Human), Q8VIJ6 (Mouse)

Gene ID

6421 (SFPQ), 71514 (Sfpq)

Available reactivities

Hu (Human), Bov (Bovine), Ms (Mouse), Rt (Rat), Can (Canine), Chk (Chicken), Ze (Zebrafish), African clawed frog, Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), Immunoprecipitation (IP)

Background of SFPQ / PSF antibody

The RNAs that direct protein synthesis in animals and plant cells are synthesized in the nucleus as large precursors (pre-mRNAs). The protein coding sequences in pre-mRNA molecules are arranged in discontinuous segments - exons interspersed with noncoding sequences - introns. In a process termed splicing, these introns are efficiently removed before the pre-mRNA is transported from the nucleus to the cytoplasm, where it is translated into protein. Studies have shown that nuclear pre-mRNA splicing takes place in a multi-component structure termed a spliceosome. The polypyrimidine tract-binding (PTB) protein-associated splicing factor (PSF), which plays an essential role in mammalian spliceosomes, is a ubiquitous nuclear matrix protein. A complex between PTB and PSF is necessary for pre-mRNA splicing. PSF contains two consensus RNA-binding domains and an unusual amino terminus rich in proline and glutamine residues. The RNA-binding properties of PSF are apparently identical to those of PTB. Both proteins, together and independently, bind the polypyrimidine tract of mammalian introns. However, the nuclear localization of PSF and PTB and their distribution in subnuclear fractions differ markedly: isolated nuclear matrices contain a bulk of PSF, but only minor amounts of PTB. In confocal microscopy both proteins appear in speckles, the majority of which do not co-localize. These PTB/PSF complexes, as well as the observed PSF-PTB interaction, may reflect the presence of PTB and PSF in spliceosomal complexes during RNA processing, although other data point to different cellular distribution and nuclear matrix association of the majority of PSF and PTB. The cleavage of PSF during lysis of immature myeloid cells is accompanied by digestion of the PTB splicing regulator but not other proteins tested. In contrast, during apoptosis PTB is degraded while PSF remains intact. Proteolytic degradation of PSF specifically occurs in intact myeloid cells and this process is enhanced upon immature myeloid cell lysis; PSF is completely cleaved to a 47 kDa proteolytic cleavage product (p47), due to potent proteolytic activity found in these cells but rare in other cells and tissues. Furthermore, p47 is abundant in intact normal and tumor myeloid cells while in other cell types it is undetectable. The bone marrow 47 kDa protein is a fragment constituting the N-terminal, protease-resistant half of the splicing factor PSF. PSF is highly basic and migrates anomalously on SDS gels. The 47 kDa protein of mouse cells of immature myeloid origin (bone marrow and acute myeloid leukemia) exhibits a gel migration pattern corresponding to a 49 kDa molecule. In other cell types such as lymphoid cells and in peripheral blood cells, PSF appears as approx. 100 kDa or 75 kDa molecules. The sequence of a fragment of mouse PSF was found to be remarkably similar to that of human PSF (> 98% homology). Also, the sequences of PSF and the human (h) 100 kDa DNA-pairing protein (hPOMp100) reveals identity. Homologous pairing is a fundamental biological reaction implicated in various cellular processes such as DNA recombination and repair, chromosome pairing, sister chromatid cohesion and chromosome condensation, gene inactivation and initiation of replication. The base pairing is also involved in spliceosome assembly resulting in formation of a dynamic Holliday-like structure within which splicing occurs. Indeed, PSF/hPOMp100 bind both singlestranded (ss) and double-stranded (ds) DNA and facilitates the renaturation of complementary ssDNA molecules. Importantly, PSF/hPOMp100 promotes the formation of D-loops in superhelical duplex DNA. PSF/hPOMp100 also serves as an efficient substrate for protein kinase C (PKC) in vitro. PKC phosphorylation of PSF/hPOMp100 stimulates its DNA binding and D-loop formation activity suggesting a possible regulatory mechanism. PSF has been demonstrated to interact with a variety of cellular targets including the human pro-oncoproteins EWS, hPOMp75/TLS and calmodulin, the RNA/DNAbinding nuclear protein p54nrb/NonO (the homolog of PSF) and DNA topoisomerase. A direct interaction has been observed, between PSF and topoisomerase I which has been implied in DNA recombination, DNA repair, and chromosome formation and may act as a transcription factor and a protein kinase. PSF is also expressed by differentiating neurons in developing mouse brain. Both the expression of PSF mRNA in cortex and cerebellum and PSF immunoreactivity in all brain areas has been found to be high during embryonic and early postnatal life. In adult tissue, only various neuronal populations in the hippocampus and olfactory bulb express PSF. PSF is expressed by differentiating neurons but not by astrocytic cells including radial glia; however oligodendrocytes differentiating in vitro were found to express it. The restricted expression of PSF suggests that it is involved in the control of neuronal-specific splicing events occurring at particular stages of neuronal differentiation and maturation. Monoclonal antibodies reacting specifically with PSF are useful tools for the molecular identification and characterization of the functional activity of PSF.

General readings

General / background references:Akhmedov AT & Lopez BS Human 100-kDa homologous DNA-pairing protein is the splicing factor PSF and promotes DNA strand invasion. Nucleic Acids Res 28:3022-30 (2000). PubMed PMID: 10931916
Chanas-Sacra G et al. Identification of PSF, the polypyrimidine tract-binding protein-associated splicing factor, as a developmentally regulated neuronal protein. J Neurosci Res 57:62-73 (1999). PubMed PMID: 10397636
Straub T et al. The RNA-splicing factor PSF/p54 controls DNA-topoisomerase I activity by a direct interaction. J Biol Chem 273:26261-4 (1998). PubMed PMID: 9756848

  1. Quick-Filters:
  2. Highly Validated (5)

16 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SFPQ / PSF antibody

WB Suggested Anti-SFPQ Antibody Titration: 1.25 ug/ml; ELISA Titer: 1: 12500; Positive Control: HepG2 cell lysate.SFPQ is strongly supported by BioGPS gene expression data to be expressed in HepG2 Rabbit IgG Purified Bov, Can, Hu, Ms, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SFPQ / PSF antibody

Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2a 6D7 Purified Hu E, ICC/IF, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

SFPQ / PSF antibody

Immunoperoxidase of monoclonal antibody to SFPQ on formalin-fixed paraffin-embedded human thyroid nodular goiter. [antibody concentration 3 ug/ml] Mouse IgG2a 6D7 Purified Hu E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SFPQ / PSF antibody

Human Intestine; Rabbit Anti-SFPQ Antibody. Catalog Number: ARP40572. Paraffin Embedded Tissue: Human Intestine. Cellular Data: Epithelial cells of intestinal villas. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X; SFPQ antibody - N-terminal region (ARP40572_T100) in Human Intestine cells using Immunohistochemistry Rabbit Aff - Purified Bov, Hu, Ms, Rt P, WB
0.1 mg / €310.00
  AVIVA Systems Biology

SFPQ / PSF antibody

Immunohistochemistry-Paraffin: SFPQ Antibody [NB100-61045] - FFPE section of human breast carcinoma.  Affinity purified rabbit anti-SFPQ used at a dilution of 1:100. Detection: DAB staining using Immunohistochemistry Accessory Kit Rabbit IgG Aff - Purified Hu, Ms ICC/IF, IP, P, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

SFPQ / PSF antibody

WB Suggested Anti-SFPQ Antibody Titration: 5.0 ug/ml; Positive Control: Jurkat cell lysate; SFPQ is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ze P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SFPQ / PSF (693-706) antibody

Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE) Goat IgG Aff - Purified Bear, Bov, Bt, Can, Chk, Elephant, Eq, Hst, Hu, Mky, Ms, Op, Platypus, Prim, Rb, Rt, Tr, Xen P
50 µg / €470.00
  Acris Antibodies GmbH

SFPQ / PSF antibody

SFPQ / PSF Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

SFPQ / PSF antibody

SFPQ monoclonal antibody (M01), clone 3B10. Western Blot analysis of SFPQ expression in Hela NE ( Cat # L013V3 ). Mouse IgG2a 3B10 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SFPQ / PSF antibody

Human Muscle; Rabbit Anti-SFPQ Antibody. Catalog Number: ARP40571. Paraffin Embedded Tissue: Human Muscle. Cellular Data: Skeletal muscle cells. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X; SFPQ antibody - middle region (ARP40571_T100) in Human Muscle cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Rt, Ze P, WB
0.1 mg / €310.00
  AVIVA Systems Biology

SFPQ / PSF antibody

WB and Immunoprecipitation: PSF/SFPQ Antibody [NB100-61043] - Detection of Human PSF/SFPQ on HeLa whole cell lysate using NB100-61043. PSF/SFPQ was also immunoprecipitated by rabbit anti-PSF/SFPQ antibodies NB100-61044 and NB100-61045. Rabbit IgG Aff - Purified Hu IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

SFPQ / PSF antibody

SFPQ / PSF Mouse IgG1 B92 Purified Hu, Ms WB
50 µl / €340.00
  Novus Biologicals Inc.

SFPQ / PSF antibody

Western Blot: SFPQ Antibody [NBP1-57303] - This Anti-SFPQ antibody was used in Western Blot of Jurkat tissue lysate at a concentration of 5ug/ml. Rabbit Purified Hu, Ms, Rt P, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

SFPQ / PSF antibody

SFPQ / PSF Rabbit Purified Hu P, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

SFPQ / PSF

SFPQ / PSF Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

SFPQ / PSF

SFPQ / PSF Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

16 Item(s)

per page
  • LinkedIn