SFRS3 antibody

Principal name

SFRS3 antibody

Alternative names for SFRS3 antibody

SRP20, Splicing factor arginine/serine-rich 3, Pre-mRNA-splicing factor SRP20

SwissProt ID

P84103 (Human), P84104 (Mouse), Q3SZR8 (Bovin)

Gene ID

6428 (SRSF3)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Dros (Drosophila), Ze (Zebrafish), African clawed frog, Eq (Equine), GP (Guinea Pig), Gt (Goat), Rb (Rabbit), Sh (Sheep)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P)

Background of SFRS3 antibody

Pre-mRNA splicing enhancer elements are short RNA sequences capable of activating weak splice sites in nearby introns that are required for accurate splice site recognition and the control of alternative splicing. Splicing enhancer elements contain specific binding sites for serine/arginine (SR)-rich splicing factors, which include SC35, 9G8, SRp20, and SF2/ASF. The family of SR factors all contain one or more RNA recognition motifs (RRM) and an arginine/ serine (RS)-rich domain. They are not only essential for constitutive splicing but also regulate splicing in a concentration-dependent manner by influencing the selection of alternative splice sites. The majority of SR proteins, including SC35 and SRp40, are confined to the nucleus, while SF2/ASF, SRp20, and 9G8 are continuously shuttled between the nucleus and the cytoplasm and contribute to mRNA transport. The activity of SR proteins in regulated splicing is antagonized by members of the hnRNP A/B family of proteins, which induce drastic shifts in the selection of splicing sites. An additional SR-associated protein, p32, tightly associates with SR factors and preferentially inhibits ASF/SF2 functioning as both a splicing enhancer and splicing repressor protein by preventing the stable interaction of ASF/SF2 and RNA.

General readings

Bedard,K.M., (2007) EMBO J. 26 (2), 459-467

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SFRS3 antibody

Immunofluorescence of monoclonal antibody to SFRS3 on HeLa cell. [antibody concentration 10 ug/ml] Mouse IgG1 2D2 Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SFRS3 antibody

Western blot analysis of extracts from HeLa cells and Jurkat cells, using SFRS3 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SFRS3 antibody

WB Suggested Anti-SFRS3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Transfected 293T Rabbit IgG Purified Bov, Can, Eq, GP, Gt, Hu, Ms, Rb, Rt, Sh, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

SFRS3 antibody

Western blot analysis of SRSF3 in K562 cell lysate with SRSF3 antibody at (A) 1 and (B) 2 ug/mL. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SFRS3 antibody

Western blot analysis of SRp20 (SFRS3) in extracts from HeLa using SFRS3 antibody (Cat.-No. AP01175PU-N). Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

SFRS3 (C-term) antibody

Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

SFRS3 (Center) antibody

Western blot analysis using SFRS3 Antibody (Center) Cat.-No AP53880PU-N in HepG2 cell line lysates (35ug/lane). This demonstrates the SFRS3 antibody detected the SFRS3 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

SFRS3 antibody

SFRS3 polyclonal antibody (A01), Lot # 060707JCS1 Western Blot analysis of SFRS3 expression in IMR-32 ( Cat # L008V1 ). Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

SFRS3 antibody

Immunofluorescence analysis of MCF7 cells, using SFRS3 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, ICC/IF, WB
50 µg / €205.00
  Assay Biotech

SFRS3 antibody

Immunofluorescence analysis of MCF7 cells, using SFRS3 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, ICC/IF, WB
0.1 mg / €310.00
  Assay Biotech

SFRS3 antibody

Transfected 293T; WB Suggested Anti-SFRS3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Transfected 293T; SFRS3 antibody - N-terminal region (ARP40438_P050) in Transfected 293T cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Dros, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

SFRS3 antibody

Immunohistochemical analysis of SRSF3 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

SFRS3 antibody

Immunohistochemical analysis of SRSF3 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

SFRS3 antibody

Immunohistochemical analysis of SRSF3 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

SFRS3 antibody

Western Blot: SRp20 Antibody [NBP1-01020] - Western blot (WB) analysis of SRp20 (F151) antibody in extracts from HeLa Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

SFRS3 antibody

Western Blot: SFRS3 Antibody [NBP1-57242] - This Anti-SFRS3 antibody was used in Western Blot of Transfected 293T tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €440.00
  Novus Biologicals Inc.

SFRS3 antibody

SFRS3 Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

SFRS3 antibody

SFRS3 Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, WB
50 µg / €290.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

SFRS3

SFRS3 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

SFRS3

SFRS3 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

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