SHC1 / SHC antibody

Principal name

SHC1 / SHC antibody

Alternative names for SHC1 / SHC antibody

SHC-transforming protein 1, SHC-transforming protein A, SHC-transforming protein 3, SHCA, Src homology 2 domain-containing-transforming protein C1

SwissProt ID

P29353 (Human), P98083 (Mouse), Q0IIE2 (Bovin), Q5M824 (Rat), Q8AY68 (Xenla)

Gene ID

6464 (SHC1)

Available reactivities

Hu (Human), Ms (Mouse), Can (Canine), Rt (Rat), Bov (Bovine), Chk (Chicken), Ze (Zebrafish), African clawed frog

Available hosts

Mouse, Rabbit

Available applications

Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Immunoprecipitation (IP), Flow Cytometry (F)

Background of SHC1 / SHC antibody

The Sch protein as targets for activated tyrosine kinases and have been implicated in the transmission of down-stream activation signals to Ras proteins. Phosphorylation of Sch protein form stable complexes with cellular tyrosine phosphorylated proteins and with the GRB2 adapter protein. At least 2 Shc isoforms have been characterized with apparent MW of 52 and 46 kDa, both share a proline and glycine rich C-terminal SH2 domain CH1 and an N-terminal phosphotyrosine binding domain (PTB). Two new Shc-related cDNa were cloned (ShcB and ShcC) that exhibit similar binding specificities in and bind to activated EGFR in a posphotyrosine dependent manner. In contrast to ShcA which is widely expressed the ShcB and ShcC are expressed in distinct brain regions and are involved in cell type specific signaling in nervous system. Conformational changes in protein-protein interactions allows the protein to perform differential regulatory functions.
The Sch adapter protein (ShcA) posses two distinct phosphotyrosine-recognition motifs, a C-terminal Src homology 2 (SH2) domain and an N-terminal PTB
domain. The ShcA undergo phosphorylation on tyrosine residues in response to many extracellular signals. The human ShcA at Tyr-317 with in its central CH1 region with two phosphorylated tyrosine-X-asparagine motifs (pYXN) motif that each binding to the Growth factor receptor bound protein 2 (GRB2) in the SH2 domain and is implicated in the activation of Ras pathway (1). Phylogenetic analysis indicates that ShcA may signalthrough both pYXN-dependent and -independent pathways. Mouse cardiomyocytes expressed ShcA directs mid-gestational heart development by a PTB-dependent mechanism that does not require the pYXN motifs. In contrast the pYXN motifs are required with the PTB and SH2 domain in the same ShcA molecule for the formation of muscle spindles, the skeletal muscle sensory organs that regulate motor behavior (2).The ShcA growth factor adapter protein translates oxidative damage in to cell death by acting as reactive oxygen species (ROS) producer with in the mitochondria. This is achieved by protein kinase C beta which is activated by oxidative conditions in the cells induces phosphorylation of SchA and triggers the mitochondrial accumulation of the protein after it is recognized by the prolyl isomerase 1 (PIN1). Once imported the SchA cuses alterations of the mitochondrial Ca+2 response and the three dimensional structure causing apoptosis (2). The increases ROS and loss o endothelial NO bioavailability also are the key feature of vascular disease in diabetes mellitus. Genetically SchA deficient mice exhibit increased resistance to ROS and prolonged life span when challenged under hyperglycemic conditions in experimental diabetes model. The salient differences between wild type and SchA-/- animals were noted in lipid peroxidation levels, ROS-dependent endothelial dysfunction, induction of heame oxygenease1 and endothelial NO synthase (3).

General readings

Trampont P, et al. (2006) Mol Cell Biol; 26(23): 9035-9044.
Patrussi L, et al. (2005) Oncogene; 24(13): 2218-2228
van der Geer P, et al. (1996) Curr Biol ; 6(11): 1435-1444

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SHC1 / SHC antibody

Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-SHC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501056, Dilution 1:50) Mouse IgG1 OTI3A1 Purified Hu ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

SHC1 / SHC antibody

Immunofluorescent staining of HT29 cells using anti-SHC1 mouse monoclonal antibody (TA501074). Mouse IgG1 OTI1D3 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SHC1 / SHC antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-SHC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501075, Dilution 1:50) Mouse IgG1 OTI1H8 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SHC1 / SHC antibody

Human Adipocytes: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse 3F4 Hu E, ICC/IF, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

SHC1 / SHC antibody

Immunoperoxidase of monoclonal antibody to SHC1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 1 ug/ml] Mouse IgG 3F4 Purified Hu E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SHC1 / SHC antibody

Western blot analysis of extracts from Hela cells treated with Calyculin A (50nM, 15min), using Shc (Ab-349) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SHC1 / SHC antibody

Predicted band size: 63 kDa . Positive control: HepG2 cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lysate: 30 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SHC1 / SHC antibody

Western blot analysis of Shc expression in CalyculinA treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SHC1 / SHC antibody

Western blot analysis of Shc expression in CalyculinA treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SHC1 / SHC antibody

Anti-SHC1 mouse monoclonal antibody (TA501103) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SHC1(RC204362). Mouse IgG1 OTI3H1 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SHC1 / SHC (C-term) (incl. pos. control) antibody

Detection of endogenous shc: Whole cell lysates of serum starved tumor cells (ca. 20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab shc-11F6 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: HeLa; lane 2: HepG2; lane 3: HEK293; lane 4: SH-SY5Y; lane 5: MDCK; lane 6: PC12; lane 7: CMT 93; lane 8: Neuro 2A; lane 9: 3T3 Mouse IgG1 11F6 Purified Hu, Ms C, ICC/IF, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

SHC1 / SHC pTyr239/240 (incl. pos. control) antibody

Phosphospecificity Whole cell extracts of control (co), EGF-stimulated (EGF) or pervanadate-treated (VH) A549 tumor cells were applied to SDS-PAGE (ca. 20.000 cells per lane) and transferred to a PVDF membrane. The immunoblot was probed with mab shc-1E3 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Mouse IgG1 1E3 Purified Can, Hu, Ms C, E, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

SHC1 / SHC pTyr317 (incl. pos. control) antibody

Phosphospecificity Whole cell extracts of control (co), EGF stimulated (EGF) or pervanadate treated (VH) A549 tumor cells were applied to SDS-PAGE (ca 20.000 cells per lane) and transferred to a PVDF membrane. The immunoblot was probed with mab shc-15E11 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Mouse IgG1 15E11 Purified Can, Hu, Ms C, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

SHC1 / SHC (N-term) (incl. pos. control) antibody

Detection of endogenous p66shc: Whole cell lysates of serum starved tumor cells (ca. 20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab shc/p66-24E4 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). 
Lane 1: HeLa
Lane 2: HepG2
Lane 3: HEK293
Lane 4: SH-SY5Y
Lane 5: MDCK
Lane 6: PC12
Lane 7: CMT 93
Lane 8: Neuro 2A
Lane 9: 3T3 Mouse IgG1 24E4 Purified Can, Hu, Ms, Rt WB
0.1 mg / €430.00
  Acris Antibodies GmbH

SHC1 / SHC pSer36 (incl. pos. control) antibody

Immunoblot Analysis: HeLa cells were cultured under serum-free conditions for 24h and subsequently stimulated with 10 ng/ml EGF. Cells were lysed with RIPA buffer and shc immunoprecipitated with polyclonal anti-shc (Transduction Labs). Immunoprecipitates were separated by SDS-PAGE. Immunoblots were developed using mab shc/p66-6E10 at 1 µg/ml. Mouse IgG1 6E10 Purified Can, Hu, Ms E, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

SHC1 / SHC pTyr349 antibody

Figure 2. Immunofluorescence staining of methanol-fixed HeLa cells using Shc1 pTyr349 antibody AP02541PU (Red). Rabbit Aff - Purified Hu, Ms ICC/IF, WB
0.1 ml / €320.00
  Acris Antibodies GmbH

SHC1 / SHC pTyr349 antibody

Figure 2. Immunofluorescence staining of methanol-fixed HeLa cells using Shc1 pTyr349 antibody AP02541PU (Red). Rabbit Aff - Purified Hu, Ms ICC/IF, WB
50 µl / €240.00
  Acris Antibodies GmbH

SHC1 / SHC pTyr427 antibody

Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using Shc1 pTyr427 antibody AP02542PU Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €320.00
  Acris Antibodies GmbH

SHC1 / SHC pTyr427 antibody

Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using Shc1 pTyr427 antibody AP02542PU Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
50 µl / €240.00
  Acris Antibodies GmbH

SHC1 / SHC antibody

Figure 2. Immunofluorescence staining of methanol-fixed HeLa cells using Shc1 antibody AP02793PU (Red). Rabbit Aff - Purified Hu, Ms ICC/IF, WB
0.1 ml / €270.00
  Acris Antibodies GmbH

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