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SIAH1 antibody

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Principal name

SIAH1 antibody

Alternative names for SIAH1 antibody

Siah-1, Siah-1a, HUMSIAH, E3 ubiquitin-protein ligase SIAH1, Seven in absentia homolog 1

SwissProt ID

Q7ZVG6 (Danre), Q8IUQ4 (Human), Q920M9 (Rat)

Gene ID

6477 (SIAH1), 140941 (Siah1a)

Ncbi ID

9606, NP_001006611, NP_003022, NP_001006611.1, NM_003031, NM_001006610, NP_003022.3, NM_080905

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Rt (Rat), Ze (Zebrafish), African clawed frog, Chk (Chicken), Dros (Drosophila), Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Por (Porcine)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Frozen Sections (C)

Background of SIAH1 antibody

Seven in absentia homolog 1 (SIAH-1) is a member of the RING-finger-containing E3 ubiquitin ligases. Alpha-synuclein and synphilin-1 are substrates of SIAH-1 , both proteins are involved in the development of Parkinson's disease (PD). Mutations in Parkin, another E3 ubiquitin ligase which ubiquinates synphilin-1 and glycosylated alpha-synuclein, have been defined as a major cause of autosomal recessive PD. The role of SIAH-1 in PD is highlighted by the fact that SIAH-1 is a component of the Lewy bodies and plays a role in apoptosis caused by nitric oxide (NO) induced oxidative stress. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) a classic glycolytic enzyme, and multi-functional protein. GAPDH plays role as a mediator for cell death. GAPDH translocates to the nucleus under a variety of stressors/conditions, most of which are associated with oxidative stress. Sequential steps lead to nuclear translocation of GAPDH during cell death; 1] a catalytic cysteine in GAPDH (C150 in rat GAPDH) is S-nitrosylated by nitric oxide (NO) which is generated from inducible nitric oxide synthase (iNOS) and/or neuronal NOS (nNOS); 2] the modified GAPDH becomes capable of binding with Siah1, an E3 ubiquitin ligase, and stabilizes it;3] the GAPDH-Siah protein complex translocates to the nucleus, dependent on Siah1's nuclear localization signal, and degrades Siah1's substrates in the nucleus, which results in cytotoxicity. GAPDH may be genetically associated with late-onset Alzheimer's disease. (-)-deprenyl, which has been used as a monoamine oxidase inhibitor for Parkinson's disease, binds to GAPDH and has neuroprotective actions. The molecular action of this protection is as yet not clear.

General readings

Susini L. et al. Siah-1 binds and regulates the function of Numb. Proc Natl Acad Sci U S A. 2001 Dec 18;98(26):15067-72.

ARP34163_T100 - SIAH1
Human Heart; . Rabbit Anti-SIAH1 antibody. . Catalog Number:. ARP34163_T100 . Paraffin Embedded Tissue: Human Heart cell . Cellular Data: Epithelial cells of renal tubule. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X; SIAH1 antibody (ARP34163_T100) in Human Heart cells using Immunohistochemistry
ARP34163_T100 - SIAH1
Human HEK293T; Researcher: Peter Brand & Dr. Oliver Kramer, Institute for Biochemistry and Biophysics, Center for Molecular Biomedicine, Friedrich-Schiller University JenaApplication: Western blottingSpecies+tissue/cell type: Human embryonic kidney 293THow many ug’s of tissue/cell lysate run on the gel:1: 50 ug human HEK-293T cell lysatePrimary antibody dilution: 1:1000Secondary antibody: Anti-rabbit-IgGSecondary antibody dilution: 1:5000 - See more at: http://www.avivasysbio.com/anti-siah1-antibody-n-terminal-region-arp34163-t100.html#sthash.GIS7dILc.dpuf. SIAH1 is strongly supported by BioGPS gene expression data to be expressed in Human HEK293T cells.; SIAH1 antibody - N-terminal region (ARP34163_T100) in Human HEK293T cells using Western Blot
ARP34163_T100 - SIAH1
Human Jurkat; WB Suggested Anti-SIAH1 Antibody Titration: 1.25ug/ml. ELISA Titer: 1:312500. Positive Control: Jurkat cell lysate; SIAH1 antibody - N-terminal region (ARP34163_T100) in Human Jurkat cells using Western Blot
ARP38212_T100 - SIAH1
Human Liver; Liver; SIAH1 antibody - C-terminal region (ARP38212_T100) in Human Liver cells using Immunohistochemistry
ARP38212_T100 - SIAH1
Human HEK293T; . Lanes: Lane1: 50 ug human HEK-293T lysate. Primary Antibody Dilution: 1:1000. Secondary Antibody: Anti-rabbit-IgG. Secondary Antibody Dilution: 1:5000. Gene Name: SIAH1. Submitted by: Peter Brand & Dr. Oliver Kramer, Institute for Biochemistry and Biophysics, Center for Molecular Biomedicine, Friedrich-Schiller University Jena. SIAH1 is strongly supported by BioGPS gene expression data to be expressed in Human HEK293T cells.; SIAH1 antibody - C-terminal region (ARP38212_T100) in Human HEK293T cells using Western Blot
ARP38212_T100 - SIAH1
Human Placenta; WB Suggested Anti-SIAH1 Antibody Titration: 1.25ug/ml. ELISA Titer: 1:312500. Positive Control: Human Placenta; SIAH1 antibody - C-terminal region (ARP38212_T100) in Human Placenta cells using Western Blot
AP05260PU-N - SIAH1
Western blot using AP05260PU-N, affinity purified rabbit polyclonal at 1 µg/ml on human brain lysate (20 µg/lane). Blots were developed with goat anti-MIg (1:30k) and Pierce's Supersignal West Femto system.
AP05260SU-N - SIAH1
Western blot using AP05260SU-N, rabbit polyclonal at 1 µg/ml on human brain lysate (20 µg/lane). Blots were developed with goat anti-MIg (1:30k) and Pierce's Supersignal West Femto system.
PAB6099 - SIAH1
SIAH1 polyclonal antibody ( Cat # PAB6099 ) staining ( 0.1 µg/ml ) of human liver lysate ( RIPA buffer, 30 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
H00006477-M02 - SIAH1
SIAH1 monoclonal antibody (M02), clone 2C5. Western Blot analysis of SIAH1 expression in Jurkat(Cat # L017V1 ).
MAB2299 - SIAH1
SIAH1/SIAH2 monoclonal antibody, clone 8G7H12 ( Cat # MAB2299 ) recognize both Drosophila SINA and human SIAH. There are two SINA-like E3 ligases, SINA and SINAH, in Drosophila.SIAH1/SIAH2 monoclonal antibody, clone 8G7H12 is highly specific in recognizing both Drosophila SINA and human SIAH proteins but not Drosophila SINAH.Coomassie blue staining patterns of both the wild-type ( WT ) and dominant negative ( PD ) forms of affinity-purified recombinant GST-SINA/SINAH/SIAH fusion proteins are shown in the top panel.Protein standards were included and are shown in kDa.Cross-reactivity of SIAH1/SIAH2 monoclonal antibody, clone 8G7H12 against Drosophila SINA and SINAH, and human hSIAH-1 and hSIAH-2 proteins were determined.SIAH1/SIAH2 monoclonal antibody, clone 8G7H12 recognizes both human SIAH-1 and SIAH-2 protein.Photo courtesy of Dr. Amy Tang, Mayo Clinic Cancer Center.
PAB10383 - SIAH1
Western blot using SIAH1 polyclonal antibody ( Cat # PAB10383 ) at 1 µg/ml on human brain lysate (20 µg/lane).
PAB8468 - SIAH1
Western blot analysis of 293T cells overexpressing human SIAH1 with MG-132 ( protease inhibitor ) , using SIAH2 polyclonal antibody ( Cat # PAB8468 ).Lane 1 : control.Lane 2 : SIAH1 gene transgenic cell treat with MG-132 ( protease inhibitor ).
AP20448PU-N - SIAH1
Western blot analysis of Siah-1/2 Antibody (Cat.-No AP20448PU-N) in extracts from HeLa cells at 1/500 dilution.
C11300-1 - SIAH1
Western blot analysis of extracts from A549/HeLa cells, using SIAH1 Antibody. The lane on the right is treated with the synthesized peptide.
AP53913PU-N - SIAH1
Western blot analysis of SIAH1 Antibody (Center) Cat.-No AP53913PU-N in human placenta tissue lysates (35ug/lane).This demonstrates the SIAH1 antibody detected the SIAH1 protein (arrow).
AR50493PU-N - SIAH1
CPA3491-100ul - SIAH1/2
Immunohistochemical analysis of SIAH1/2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
CPA3491-100ul - SIAH1/2
Immunofluorescent analysis of SIAH1/2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
CPA3491-100ul - SIAH1/2
Western blot analysis of SIAH1/2 expression in HeLa (A); Jurkat (B) whole cell lysates.
TA302689 - SIAH1
Antibody staining (0.1µg/ml) of Human Liver lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
TA302689 - SIAH1
Antibody (1μg/ml) staining of Mouse (A, C) and Rat (B, D) Brain (A, B) and Liver (C, D) lysates (35μg protein in
RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA332521 - SIAH1
Western blot analysis of extracts of various cell lines, using SIAH1 antibody.
TA332521 - SIAH1
Immunofluorescence analysis of U2OS cell using SIAH1 antibody. Blue: DAPI for nuclear staining.
TA334774 - SIAH1
WB Suggested Anti-SIAH1 Antibody Titration: 1.25 ug/ml; ELISA Titer: 1:312500; Positive Control: Jurkat cell lysate
TA334774 - SIAH1
Researcher: Peter Brand & Dr. Oliver Kramer, Institute for Biochemistry and Biophysics, Center for Molecular Biomedicine, Friedrich-Schiller University JenaApplication: Western blottingSpecies+tissue/cell type: Human embryonic kidney 293THow many ug�s o
TA334774 - SIAH1
Rabbit Anti-SIAH1 antibody; Paraffin Embedded Tissue: Human Heart cell; Cellular Data: Epithelial cells of renal tubule; Antibody Concentration: 4.0-8.0 ug/ml; Magnification: 400X
TA335795 - SIAH1
WB Suggested Anti-SIAH1 Antibody Titration: 1.25 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human Placenta
TA335795 - SIAH1
Lanes: Lane1: 50 ug human HEK-293T lysate; Primary Antibody Dilution: 1: 1000; Secondary Antibody: Anti-rabbit-IgG; Secondary Antibody Dilution: 1: 5000; Gene Name: SIAH1; Submitted by: Peter Brand & Dr. Oliver Kramer, Institute for Biochemistry and Biophysics, Center for Molecular Biomedicine, Friedrich-Schiller University Jena; SIAH1 is strongly supported by BioGPS gene expression data to be expressed in Human HEK293T cells
TA335795 - SIAH1
Liver
TA336573 - SIAH1
Western Blot: SIAH1/2 Antibody (8G7H12) [TA336573] - Anti-SINA/SIAH monoclonal antibodies recognize both Drosophila SINA and human SIAH. There are two SINA-like E3 ligases, SINA and SINAH, in Drosophila. SINA monoclonal antibody 8G7H12 is highly speci
TA350414 - SIAH1
Gel: 12%SDS-PAGE, Lysate: 40 ug, Lane 1-4: Hela, Jurkat and hepg2 cell, human fetal liver tissue, Primary antibody: (SIAH1 Antibody) at dilution 1/300 dilution, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute
TA350414 - SIAH1
The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using (SIAH1 Antibody) at dilution 1/35, on the right is treated with fusion protein. (Original magnification: ×200)
TA350414 - SIAH1
The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using (SIAH1 Antibody) at dilution 1/35, on the right is treated with fusion protein. (Original magnification: ×200)
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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SIAH1 (N-term) antibody
  Goat Polyclonal Antibody against SIAH1  
Antibody staining (0.1µg/ml) of Human Liver lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt E, IP, WB
0.1 mg / €360.00
  OriGene Technologies, Inc.
+1 additional image

Published recently!

SIAH1 antibody
  Rabbit anti-SIAH1 Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using SIAH1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €335.00
  OriGene Technologies, Inc.
+1 additional image

SIAH1 antibody
  Rabbit Polyclonal Anti-SIAH1 Antibody  
WB Suggested Anti-SIAH1 Antibody Titration: 1.25 ug/ml; ELISA Titer: 1:312500; Positive Control: Jurkat cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rt P, WB
0.1 mg / €360.00
  OriGene Technologies, Inc.
+2 additional images

SIAH1 antibody
  Rabbit Polyclonal Anti-SIAH1 Antibody  
WB Suggested Anti-SIAH1 Antibody Titration: 1.25 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human Placenta Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Rb, Rt P, WB
0.1 mg / €360.00
  OriGene Technologies, Inc.
+2 additional images

SIAH1 antibody
  Mouse Monoclonal SIAH1/2 Antibody (8G7H12)  
Western Blot: SIAH1/2 Antibody (8G7H12) [TA336573] - Anti-SINA/SIAH monoclonal antibodies recognize both Drosophila SINA and human SIAH. There are two SINA-like E3 ligases, SINA and SINAH, in Drosophila. SINA monoclonal antibody 8G7H12 is highly speci Mouse IgG1 8G7H12 Purified Dros, Hu, Ms, Por, Rt, Ze IP, WB
0.5 ml / €393.00
  OriGene Technologies, Inc.

SIAH1 antibody
  Rabbit Polyclonal Anti-SIAH1 Antibody  
Gel: 12%SDS-PAGE, Lysate: 40 ug, Lane 1-4: Hela, Jurkat and hepg2 cell, human fetal liver tissue, Primary antibody: (SIAH1 Antibody) at dilution 1/300 dilution, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.
+2 additional images

SIAH1 Control Peptide
SIAH1 Purified
50 µg / €220.00
  Acris Antibodies GmbH

SIAH1 antibody
Western blot using AP05260PU-N, affinity purified rabbit polyclonal at 1 µg/ml on human brain lysate (20 µg/lane). Blots were developed with goat anti-MIg (1:30k) and Pierce's Supersignal West Femto system. Rabbit IgG Aff - Purified Hu WB
50 µg / €370.00
  Acris Antibodies GmbH

SIAH1 antibody
Western blot using AP05260SU-N, rabbit polyclonal at 1 µg/ml on human brain lysate (20 µg/lane). Blots were developed with goat anti-MIg (1:30k) and Pierce's Supersignal West Femto system. Rabbit IgG Purified Hu WB
0.1 mg / €370.00
  Acris Antibodies GmbH

SIAH1 (+SIAH2) antibody
Western blot analysis of Siah-1/2 Antibody (Cat.-No AP20448PU-N) in extracts from HeLa cells at 1/500 dilution. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

SIAH1 (Center) antibody
Western blot analysis of SIAH1 Antibody (Center) Cat.-No AP53913PU-N in human placenta tissue lysates (35ug/lane).This demonstrates the SIAH1 antibody detected the SIAH1 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

SIAH1 antibody
SIAH1 Rabbit Serum Hu C, E, WB
0.2 ml / €400.00
  Acris Antibodies GmbH

SIAH1 antibody
Western blot analysis of extracts from A549/HeLa cells, using SIAH1 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €230.00
  Assay Biotech

SIAH1 antibody
Western blot analysis of extracts from A549/HeLa cells, using SIAH1 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €330.00
  Assay Biotech

CRISPR

Catalog No. Species Donor Vector  

SIAH1 - human gene knockout kit via CRISPR
SIAH1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

SIAH1 - human gene knockout kit via CRISPR
SIAH1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

SIAH1 - human gene knockout kit via CRISPR
SIAH1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

SIAH1 - human gene knockout kit via CRISPR
SIAH1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

SIAH1 (90-282, His-tag)
SIAH1 Human Purified > 95 % by SDS - PAGE E. coli
0.25 mg / €940.00
  Acris Antibodies GmbH

SIAH1 (90-282, His-tag)
SIAH1 Human Purified > 95 % by SDS - PAGE E. coli
50 µg / €370.00
  Acris Antibodies GmbH

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