SIAH1 antibody

Principal name

SIAH1 antibody

Alternative names for SIAH1 antibody

Siah-1, Siah-1a, HUMSIAH, E3 ubiquitin-protein ligase SIAH1, Seven in absentia homolog 1

SwissProt ID

Q7ZVG6 (Danre), Q8IUQ4 (Human), Q920M9 (Rat)

Gene ID

6477 (SIAH1), 140941 (Siah1a)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Rt (Rat), Ze (Zebrafish), African clawed frog, Chk (Chicken), Dros (Drosophila), Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Por (Porcine)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Frozen Sections (C)

Background of SIAH1 antibody

Seven in absentia homolog 1 (SIAH-1) is a member of the RING-finger-containing E3 ubiquitin ligases. Alpha-synuclein and synphilin-1 are substrates of SIAH-1 , both proteins are involved in the development of Parkinson's disease (PD). Mutations in Parkin, another E3 ubiquitin ligase which ubiquinates synphilin-1 and glycosylated alpha-synuclein, have been defined as a major cause of autosomal recessive PD. The role of SIAH-1 in PD is highlighted by the fact that SIAH-1 is a component of the Lewy bodies and plays a role in apoptosis caused by nitric oxide (NO) induced oxidative stress. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) a classic glycolytic enzyme, and multi-functional protein. GAPDH plays role as a mediator for cell death. GAPDH translocates to the nucleus under a variety of stressors/conditions, most of which are associated with oxidative stress. Sequential steps lead to nuclear translocation of GAPDH during cell death; 1] a catalytic cysteine in GAPDH (C150 in rat GAPDH) is S-nitrosylated by nitric oxide (NO) which is generated from inducible nitric oxide synthase (iNOS) and/or neuronal NOS (nNOS); 2] the modified GAPDH becomes capable of binding with Siah1, an E3 ubiquitin ligase, and stabilizes it;3] the GAPDH-Siah protein complex translocates to the nucleus, dependent on Siah1's nuclear localization signal, and degrades Siah1's substrates in the nucleus, which results in cytotoxicity. GAPDH may be genetically associated with late-onset Alzheimer's disease. (-)-deprenyl, which has been used as a monoamine oxidase inhibitor for Parkinson's disease, binds to GAPDH and has neuroprotective actions. The molecular action of this protection is as yet not clear.

General readings

Susini L. et al. Siah-1 binds and regulates the function of Numb. Proc Natl Acad Sci U S A. 2001 Dec 18;98(26):15067-72.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SIAH1 (N-term) antibody

  Goat Polyclonal Antibody against SIAH1  
Antibody staining (0.1µg/ml) of Human Liver  lysate (RIPA buffer, 30µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt E, IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SIAH1 antibody

  Rabbit anti-SIAH1 Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using SIAH1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

SIAH1 antibody

  Rabbit Polyclonal Anti-SIAH1 Antibody  
WB Suggested Anti-SIAH1 Antibody Titration: 1.25 ug/ml; ELISA Titer: 1:312500; Positive Control: Jurkat cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SIAH1 antibody

  Rabbit Polyclonal Anti-SIAH1 Antibody  
WB Suggested Anti-SIAH1 Antibody Titration: 1.25 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human Placenta Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Rb, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SIAH1 antibody

  Mouse Monoclonal SIAH1/2 Antibody (8G7H12)  
Western Blot: SIAH1/2 Antibody (8G7H12) [TA336573] - Anti-SINA/SIAH monoclonal antibodies recognize both Drosophila SINA and human SIAH. There are two SINA-like E3 ligases, SINA and SINAH, in Drosophila. SINA monoclonal antibody 8G7H12 is highly speci Mouse IgG1 8G7H12 Purified Dros, Hu, Ms, Por, Rt, Ze IP, WB
0.5 ml / €325.00
  OriGene Technologies, Inc.

SIAH1 antibody

  Rabbit Polyclonal Anti-SIAH1 Antibody  
Gel: 12%SDS-PAGE, Lysate: 40 ug, Lane 1-4: Hela, Jurkat and hepg2 cell, human fetal liver tissue, Primary antibody: (SIAH1 Antibody) at dilution 1/300 dilution, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SIAH1 Control Peptide

SIAH1 Purified
50 µg / €220.00
  Acris Antibodies GmbH

SIAH1 antibody

Western blot using AP05260PU-N, affinity purified rabbit polyclonal at 1 µg/ml on human brain lysate (20 µg/lane). Blots were developed with goat anti-MIg (1:30k) and Pierce's Supersignal West Femto system. Rabbit IgG Aff - Purified Hu WB
50 µg / €490.00
  Acris Antibodies GmbH

SIAH1 antibody

Western blot using AP05260SU-N, rabbit polyclonal at 1 µg/ml on human brain lysate (20 µg/lane). Blots were developed with goat anti-MIg (1:30k) and Pierce's Supersignal West Femto system. Rabbit IgG Purified Hu WB
0.1 mg / €380.00
  Acris Antibodies GmbH

SIAH1 (+SIAH2) antibody

Western blot analysis of Siah-1/2 Antibody (Cat.-No AP20448PU-N) in extracts from HeLa cells at 1/500 dilution. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

SIAH1 (Center) antibody

Western blot analysis of SIAH1 Antibody (Center) Cat.-No AP53913PU-N in human placenta tissue lysates (35ug/lane).This demonstrates the SIAH1 antibody detected the SIAH1 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

SIAH1 antibody

SIAH1 Rabbit Serum Hu C, E, WB
0.2 ml / €400.00
  Acris Antibodies GmbH

SIAH1 antibody

Western blot analysis of extracts from A549/HeLa cells, using SIAH1 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €230.00
  Assay Biotech

SIAH1 antibody

Western blot analysis of extracts from A549/HeLa cells, using SIAH1 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €330.00
  Assay Biotech

CRISPR

Catalog No. Species Donor Vector  

SIAH1 - human gene knockout kit via CRISPR

SIAH1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

SIAH1 - human gene knockout kit via CRISPR

SIAH1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

SIAH1 - human gene knockout kit via CRISPR

SIAH1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

SIAH1 - human gene knockout kit via CRISPR

SIAH1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

SIAH1 (90-282, His-tag)

SIAH1 Human Purified > 95 % by SDS - PAGE E. coli
0.25 mg / €940.00
  Acris Antibodies GmbH

SIAH1 (90-282, His-tag)

SIAH1 Human Purified > 95 % by SDS - PAGE E. coli
50 µg / €370.00
  Acris Antibodies GmbH

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