SIAH1 antibody
Principal name
SIAH1 antibody
Alternative names for SIAH1 antibody
Siah-1, Siah-1a, HUMSIAH, E3 ubiquitin-protein ligase SIAH1, Seven in absentia homolog 1
Ncbi ID
9606, NP_001006611, NP_003022, NP_001006611.1, NM_003031, NM_001006610, NP_003022.3, NM_080905
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Frozen Sections (C)
Background of SIAH1 antibody
Seven in absentia homolog 1 (SIAH-1) is a member of the RING-finger-containing E3 ubiquitin ligases. Alpha-synuclein and synphilin-1 are substrates of SIAH-1 , both proteins are involved in the development of Parkinson's disease (PD). Mutations in Parkin, another E3 ubiquitin ligase which ubiquinates synphilin-1 and glycosylated alpha-synuclein, have been defined as a major cause of autosomal recessive PD. The role of SIAH-1 in PD is highlighted by the fact that SIAH-1 is a component of the Lewy bodies and plays a role in apoptosis caused by nitric oxide (NO) induced oxidative stress. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) a classic glycolytic enzyme, and multi-functional protein. GAPDH plays role as a mediator for cell death. GAPDH translocates to the nucleus under a variety of stressors/conditions, most of which are associated with oxidative stress. Sequential steps lead to nuclear translocation of GAPDH during cell death; 1] a catalytic cysteine in GAPDH (C150 in rat GAPDH) is S-nitrosylated by nitric oxide (NO) which is generated from inducible nitric oxide synthase (iNOS) and/or neuronal NOS (nNOS); 2] the modified GAPDH becomes capable of binding with Siah1, an E3 ubiquitin ligase, and stabilizes it;3] the GAPDH-Siah protein complex translocates to the nucleus, dependent on Siah1's nuclear localization signal, and degrades Siah1's substrates in the nucleus, which results in cytotoxicity. GAPDH may be genetically associated with late-onset Alzheimer's disease. (-)-deprenyl, which has been used as a monoamine oxidase inhibitor for Parkinson's disease, binds to GAPDH and has neuroprotective actions. The molecular action of this protection is as yet not clear.
General readings
Susini L. et al. Siah-1 binds and regulates the function of Numb. Proc Natl Acad Sci U S A. 2001 Dec 18;98(26):15067-72.


















RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.








- Quick-Filters:
- Cited (1)
Primary Antibodies
Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
---|---|---|---|---|---|---|---|
TA302689 | SIAH1 (N-term) antibody |
||||||
Goat Polyclonal Antibody against SIAH1 | |||||||
![]() |
Goat | Aff - Purified | Bov, Can, Hu, Ms, Rt | E, IP, WB |
0.1 mg /
€360.00
|
||
OriGene Technologies, Inc. | |||||||
![]() |
Published recently! |
||||||
TA332521 | SIAH1 antibody |
||||||
Rabbit anti-SIAH1 Polyclonal Antibody | |||||||
![]() |
Rabbit | IgG | Purified | Hu, Ms, Rt |
0.1 mg /
€335.00
|
||
OriGene Technologies, Inc. | |||||||
![]() |
|||||||
TA334774 | SIAH1 antibody |
||||||
Rabbit Polyclonal Anti-SIAH1 Antibody | |||||||
![]() |
Rabbit | IgG | Purified | Bov, Can, Eq, GP, Hu, Ms, Rt | P, WB |
0.1 mg /
€360.00
|
|
OriGene Technologies, Inc. | |||||||
![]() |
|||||||
TA335795 | SIAH1 antibody |
||||||
Rabbit Polyclonal Anti-SIAH1 Antibody | |||||||
![]() |
Rabbit | IgG | Purified | Bov, Can, Eq, Hu, Ms, Rb, Rt | P, WB |
0.1 mg /
€360.00
|
|
OriGene Technologies, Inc. | |||||||
![]() |
|||||||
TA336573 | SIAH1 antibody |
||||||
Mouse Monoclonal SIAH1/2 Antibody (8G7H12) | |||||||
![]() |
Mouse | IgG1 | 8G7H12 | Purified | Dros, Hu, Ms, Por, Rt, Ze | IP, WB |
0.5 ml /
€393.00
|
OriGene Technologies, Inc. | |||||||
TA350414 | SIAH1 antibody |
||||||
Rabbit Polyclonal Anti-SIAH1 Antibody | |||||||
![]() |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
OriGene Technologies, Inc. | |||||||
![]() |
|||||||
AP05260CP-N | SIAH1 Control Peptide |
||||||
![]() |
Purified |
50 µg /
€220.00
|
|||||
Acris Antibodies GmbH | |||||||
AP05260PU-N | SIAH1 antibody |
||||||
![]() |
Rabbit | IgG | Aff - Purified | Hu | WB |
50 µg /
€370.00
|
|
Acris Antibodies GmbH | |||||||
AP05260SU-N | SIAH1 antibody |
||||||
![]() |
Rabbit | IgG | Purified | Hu | WB |
0.1 mg /
€370.00
|
|
Acris Antibodies GmbH | |||||||
AP20448PU-N | SIAH1 (+SIAH2) antibody |
||||||
![]() |
Rabbit | Aff - Purified | Hu, Ms, Rt | ICC/IF, P, WB |
0.1 mg /
€350.00
|
||
Acris Antibodies GmbH | |||||||
AP53913PU-N | SIAH1 (Center) antibody |
||||||
![]() |
Rabbit | Ig | Aff - Purified | Hu | WB |
0.4 ml /
€370.00
|
|
Acris Antibodies GmbH | |||||||
AP54905SU-N | SIAH1 antibody |
||||||
![]() |
Rabbit | Serum | Hu | C, E, WB |
0.2 ml /
€400.00
|
||
Acris Antibodies GmbH | |||||||
C11300-1 | SIAH1 antibody |
||||||
![]() |
Rabbit | Aff - Purified | Hu, Ms, Rt | E, WB |
50 µg /
€230.00
|
||
Assay Biotech | |||||||
C11300-2 | SIAH1 antibody |
||||||
![]() |
Rabbit | Aff - Purified | Hu, Ms, Rt | E, WB |
0.1 mg /
€330.00
|
||
Assay Biotech |
CRISPR
Catalog No. | Species | Donor Vector | |
---|---|---|---|
KN206576 | SIAH1 - human gene knockout kit via CRISPR |
||
![]() |
Human | GFP-Puro |
1 kit /
€1,290.00
|
OriGene Technologies, Inc. | |||
KN206576BN | SIAH1 - human gene knockout kit via CRISPR |
||
![]() |
Human | mBFP-Neo |
1 kit /
€1,290.00
|
OriGene Technologies, Inc. | |||
KN206576LP | SIAH1 - human gene knockout kit via CRISPR |
||
![]() |
Human | Luciferase-Puro |
1 kit /
€1,290.00
|
OriGene Technologies, Inc. | |||
KN206576RB | SIAH1 - human gene knockout kit via CRISPR |
||
![]() |
Human | RFP-BSD |
1 kit /
€1,290.00
|
OriGene Technologies, Inc. |
Proteins & Growth Factors
Catalog No. | Species | Pres. | Purity | Source | |
---|---|---|---|---|---|
AR50493PU-N | SIAH1 (90-282, His-tag) |
||||
![]() |
Human | Purified | > 95 % by SDS - PAGE | E. coli |
0.25 mg /
€940.00
|
Acris Antibodies GmbH | |||||
AR50493PU-S | SIAH1 (90-282, His-tag) |
||||
![]() |
Human | Purified | > 95 % by SDS - PAGE | E. coli |
50 µg /
€370.00
|
Acris Antibodies GmbH |