SIAH2 antibody

Principal name

SIAH2 antibody

Alternative names for SIAH2 antibody

Siah-2, E3 ubiquitin-protein ligase SIAH2, Seven in absentia homolog 2

SwissProt ID

O43255 (Human), Q06986 (Mouse), Q7SYL3 (Danre), Q8R4T2 (Rat), Q9I8X5 (Xenla)

Gene ID

6478 (SIAH2), 20439 (Siah2), 140593

Available reactivities

Hu (Human), Bov (Bovine), Chk (Chicken), Dros (Drosophila), Ms (Mouse), Rt (Rat), Ze (Zebrafish), African clawed frog, Can (Canine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Por (Porcine)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Flow Cytometry (F), Western blot / Immunoblot (WB)

Background of SIAH2 antibody

SIAH-2 (seven in absentia homolog 2) is an E3 ligase that catalyzes ubiquitination and proteasome-mediated degradation of protein substrates. SIAH-2 encodes a 324 amino acid protein that shares 77% identity with human SIAH-1 and 68% identity with the Drosophila sina (seven in absentia) gene, on which development of the Drosophila R7 photoreceptor is dependent. SIAH-2 targets TRAF2 (which regulates cell responses to stress and cytokines through the regulation of key stress-signaling cascades) for degradation under stress conditions such as hypoxia. It targets HIF-1alpha prolyl hydroxylase 3 (PHD3) for degradation upon exposure to hypoxic conditions, which coincides with an increase in SIAH-2 transcription. SIAH-2 can decrease TNF-alpha-dependent induction of JNK activity and transcriptional activation of NFkappaB. SIAH-2 null mice subjected to hypoxia display an impaired respiratory response and reduced levels of hemoglobin.

General readings

Fiucci G. et al. Siah-1b is a direct transcriptional target of p53: identification of the functional p53 responsive element in the siah-1b promoter.Proc Natl Acad Sci U S A. 2004;101(10):3510-5.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SIAH2 antibody

  Rabbit Polyclonal Anti-SIAH2 Antibody - N-terminal region  
Lanes: 1: 50ug HEK293T lysate, 2: 50ug A549 lysate; Primary Antibody Dilution: 1:1000; Secondary Antibody: Anti-rabbit-HRP; Secondary Antibody Dilution: 1:5000; Gene Name: SIAH2; Submitted by: Peter Brand & Dr. Oliver Krammer, Institute for Biotechnology Rabbit IgG Purified Bov, Hu, Ms, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

SIAH2 antibody

  Rabbit polyclonal anti-SIAH2 antibody  
Western blot analysis of extracts from COLO cells, using SIAH2 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SIAH2 antibody

  Rabbit Polyclonal Anti-SIAH2 Antibody  
WB Suggested Anti-SIAH2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Human Muscle Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

SIAH2 antibody

  Mouse Monoclonal SIAH2 Antibody (24E6H3)  
Immunohistochemistry: SIAH2 Antibody (24E6H3) [TA336562] - Staining of SIAH in human colorectal adenocarcinoma using TA336562. Staining of SIAH was not observed in normal colon epithelium. Photos courtesy of Dr. Amy Tang, Mayo Clinic Cancer Center. Mouse IgG1 24E6H3 Purified Dros, Hu, Ms, Por C, F, ICC/IF, P, WB
0.5 ml / €325.00
  OriGene Technologies, Inc.

SIAH2 antibody

  Rabbit Polyclonal Anti-SIAH2 Antibody  
The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using (SIAH2 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: ×200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

SIAH2 antibody

Immunohistochemistry (IHC) analyzes of Siah-2 antibody (Cat.-No.: AP20493PU-N) in paraffin-embedded human heart tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

SIAH2 antibody

SIAH2 Rabbit Serum Hu C, E, WB
0.2 ml / €400.00
  Acris Antibodies GmbH

SIAH2 antibody

Western blot analysis of extracts from COLO cells, using SIAH2 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €230.00
  Assay Biotech

SIAH2 antibody

Western blot analysis of extracts from COLO cells, using SIAH2 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €330.00
  Assay Biotech

CRISPR

Catalog No. Species Donor Vector  

SIAH2 - human gene knockout kit via CRISPR

SIAH2 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

SIAH2 - human gene knockout kit via CRISPR

SIAH2 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

SIAH2 - human gene knockout kit via CRISPR

SIAH2 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

SIAH2 - human gene knockout kit via CRISPR

SIAH2 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Siah2 - mouse gene knockout kit via CRISPR

Siah2 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Siah2 - mouse gene knockout kit via CRISPR

Siah2 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Siah2 - mouse gene knockout kit via CRISPR

Siah2 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Siah2 - mouse gene knockout kit via CRISPR

Siah2 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

SIAH2

  Recombinant protein of human seven in absentia homolog 2 (Drosophila) (SIAH2)  
SIAH2 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

SIAH2 overexpression lysate

SIAH2 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, SIAH2 (mGFP-tagged) - Human seven in absentia homolog 2 (Drosophila) (SIAH2), 200 uL, >10^7 TU/mL

Lenti ORF particles, SIAH2 (mGFP-tagged) - Human seven in absentia homolog 2 (Drosophila) (SIAH2), 200 uL, >10^7 TU/mL  
200 µl / €960.00
  OriGene Technologies, Inc.

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