SIAH2 antibody

Principal name

SIAH2 antibody

Alternative names for SIAH2 antibody

Siah-2, E3 ubiquitin-protein ligase SIAH2, Seven in absentia homolog 2

SwissProt ID

O43255 (Human), Q06986 (Mouse), Q7SYL3 (Danre), Q8R4T2 (Rat), Q9I8X5 (Xenla)

Gene ID

6478 (SIAH2), 56478 (EIF4ENIF1)

Available reactivities

Hu (Human), Dros (Drosophila), Por (Porcine), Ms (Mouse), Rt (Rat), Bov (Bovine), Chk (Chicken), Ze (Zebrafish), African clawed frog, Can (Canine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Paraffin Sections (P), Western blot / Immunoblot (WB)

Background of SIAH2 antibody

SIAH-2 (seven in absentia homolog 2) is an E3 ligase that catalyzes ubiquitination and proteasome-mediated degradation of protein substrates. SIAH-2 encodes a 324 amino acid protein that shares 77% identity with human SIAH-1 and 68% identity with the Drosophila sina (seven in absentia) gene, on which development of the Drosophila R7 photoreceptor is dependent. SIAH-2 targets TRAF2 (which regulates cell responses to stress and cytokines through the regulation of key stress-signaling cascades) for degradation under stress conditions such as hypoxia. It targets HIF-1alpha prolyl hydroxylase 3 (PHD3) for degradation upon exposure to hypoxic conditions, which coincides with an increase in SIAH-2 transcription. SIAH-2 can decrease TNF-alpha-dependent induction of JNK activity and transcriptional activation of NFkappaB. SIAH-2 null mice subjected to hypoxia display an impaired respiratory response and reduced levels of hemoglobin.

General readings

Schmidt, et. al. Can. Res. 67(24):11798-11810 (2007) [Immunohistochemistry, Immunofluorescence, FACS, Western blot]

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SIAH2 antibody

Lanes: 1: 50ug HEK293T lysate, 2: 50ug A549 lysate; Primary Antibody Dilution: 1:1000; Secondary Antibody: Anti-rabbit-HRP; Secondary Antibody Dilution: 1:5000; Gene Name: SIAH2; Submitted by: Peter Brand & Dr. Oliver Krammer, Institute for Biotechnology Rabbit IgG Purified Bov, Hu, Ms, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

SIAH2 antibody

Human HepG2; Host: Rabbit .  Target Name: SIAH2 .  Sample Tissue: HepG2 . Antibody Dilution: 1.0ug/ml. SIAH2 is supported by BioGPS gene expression data to be expressed in HepG2.; SIAH2 antibody - N-terminal region (ARP58071_P050) in Human HepG2 cells using Western Blot Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

SIAH2 antibody

Western blot analysis of extracts from COLO cells, using SIAH2 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SIAH2 antibody

WB Suggested Anti-SIAH2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Human Muscle Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

SIAH2 antibody

Immunohistochemistry: SIAH2 Antibody (24E6H3) [TA336562] - Staining of SIAH in human colorectal adenocarcinoma using TA336562. Staining of SIAH was not observed in normal colon epithelium. Photos courtesy of Dr. Amy Tang, Mayo Clinic Cancer Center. Mouse IgG1 24E6H3 Purified Dros, Hu, Ms, Por C, F, ICC/IF, P, WB
0.5 ml / €325.00
  OriGene Technologies, Inc.

SIAH2 antibody

Immunohistochemistry (IHC) analyzes of Siah-2 antibody (Cat.-No.: AP20493PU-N) in paraffin-embedded human heart tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

SIAH2 antibody

SIAH2 Rabbit Serum Hu C, E, WB
0.2 ml / €400.00
  Acris Antibodies GmbH

SIAH2 antibody

SIAH2 Mouse IgG2b 1F5 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

SIAH2 antibody

Immunohistochemical staining of SIAH2 on human colorectal adenocarcinoma with SIAH2 monoclonal antibody, clone 24E6H3 ( Cat # MAB2298 ). Staining of SIAH was not observed in normal colon epithelium. Photos courtesy of Dr. Amy Tang, Mayo Clinic Cancer Center. Mouse IgG1 24E6H3 Hu C, F, ICC/IF, P
0.5 ml / €470.00
  Abnova Taiwan Corp.

SIAH2 antibody

Western blot analysis of 293T cells overexpressing human SIAH2 with MG-132 ( protease inhibitor ) , using SIAH2 polyclonal antibody ( Cat # PAB8469 ) . Rabbit Hu WB
25 µg / €660.00
  Abnova Taiwan Corp.

SIAH2 antibody

Western blot analysis of extracts from COLO cells, using SIAH2 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech

SIAH2 antibody

Western blot analysis of extracts from COLO cells, using SIAH2 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €310.00
  Assay Biotech

SIAH2 antibody

Human 721_B; Host:Rabbit. Target Name:SIAH2. Sample Tissue:721_B. Antibody Dilution: 1.0ug/ml. SIAH2 is supported by BioGPS gene expression data to be expressed in 721_B.; SIAH2 antibody - middle region (ARP33290_P050) in Human 721_B cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Chk, Dros, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

SIAH2 antibody

Immunohistochemical analysis of SIAH2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Ms, Rt, Ze ICC/IF, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

SIAH2 antibody

Immunohistochemical analysis of SIAH2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Ms, Rt, Ze ICC/IF, P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

SIAH2 antibody

Immunohistochemical analysis of SIAH2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Ms, Rt, Ze ICC/IF, P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

SIAH2 antibody

SIAH2 Rabbit Aff - Purified Hu WB
25 µg / €630.00
  Funakoshi Co., Ltd.

SIAH2 antibody

SIAH2 Mouse IgG1 24E6H3 Supernatant Dros, Hu, Por C, F, ICC/IF, P
0.125 ml / €170.00
  Novus Biologicals Inc.

SIAH2 antibody

Immunohistochemistry: Siah-2 Antibody [NBP1-19648] -  Siah-2 Antibody [NBP1-19648] - paraffin-embedded human heart tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

SIAH2 antibody

SIAH2 Rabbit IgG Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

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