Sialidase-2 antibody

Principal name

Sialidase-2 antibody

Alternative names for Sialidase-2 antibody

Cytosolic sialidase, N-acetyl-alpha-neuraminidase 2, NEU2

SwissProt ID

Q64627 (Rat), Q6NTB4 (Human), Q9JMH3 (Mouse), Q9Y3R4 (Human)

Gene ID

4759 (NEU2)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Por (Porcine)

Available hosts

Rabbit, Mouse

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F)

Background of Sialidase-2 antibody

Neuraminidases or sialidases are exoglycosidases that catalyze the cleavage of ?-glycosidically linked terminal N-acetyl neuraminic acid from sialylated glycoconjugates. They are widely spread in nature, occurring in viruses, bacteria, fungi, protozoa, birds and mammals. Together, the neuraminidases form a family of hydrolases that share a conserved active site and similar sequence motifs. Three types of neuraminidase are found in mammals and are defined as lysosomal, plasma membrane and cytosolic on the basis of their biochemical properties and subcellular distribution. Lysosomal N-cetylneuraminidase (NEU1) has significant primary structurecharacteristics of other mammalian and microbial sialidases with similar substrate specificity. However, unlike other members of this family, lysosomal neuraminidase requires the carboxypeptidase protective protein/cathepsin A (PPCA) for intracellular transport and lysosomal activation. The enzyme is only catalytically active when it is bound to PPCA and is a component of a high molecular weight, multi-protein complex containing PPCA, ß-galactosidase and N-acetylgalactosamine-6-sulfate sulfatase. Using a hamster Sial3 probe, Monti et al. (1999) identified the gene encoding sialidase-2, which they designated NEU2, from a human genomic library.
The 2 putative exons of NEU2 encode a deduced 380-amino acid protein with a calculated molecular mass of 42.23 kD. The NEU2 protein has significant homology with the mammalian, viral, and bacterial sialidases. It shares over 72% similarity with the hamster and rat cytosolic sialidases and over 42% similarity with human NEU1. NEU2 contains a potential N-linked glycosylation site, 2 aspartic acid block consensus sequences, and an N-terminal F/YRIP sequence motif which is part of the active site of other sialidase enzymes. Monti et al. hypothesized that NEU2 has a cytosolic localization because it does not contain a cleavage site, transmembrane domain, or targeting motifs.

General readings

1. Monti,E., Preti,A., Rossi,E., Ballabio,A. and Borsani,G. (1999) Cloning and characterization of NEU2, a human gene homologous to rodent soluble sialidases. Genomics 57 (1), 137-143.
2. Miyagi,T., Konno,K., Emori,Y., Kawasaki,H., Suzuki,K., Yasui,A. and Tsuik,S. (1993) Molecular cloning and expression of cDNA encoding rat skeletal muscle cytosolic sialidase. J. Biol. Chem. 268 (35), 26435-26440.
3. Fanzani A, et al. (2003) Overexpression of cytosolic sialidase Neu2 induces myoblast differentiation in C2C12 cells. FEBS Lett 547, 183-188.
4. Tringali C, et al. (2004) Properties of Recombinant Human Cytosolic Sialidase HsNEU2: THE ENZYME HYDROLYZES MONOMERICALLY DISPERSED GM1 GANGLIOSIDE MOLECULES. J Biol Chem 279, 3169-3179.
Related Link(s): LocusLink ID: 4759 OMIM Accession: 605528 UCSC Genome Browser ID: NM_005383 Ensembl ID: NEU2 GenBank ID:Y16535 Gen

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Sialidase-2 antibody

WB analysis using Immunochemical's Anti-Neu2 antibody to detect recombinant His tagged Neu-2 (1.0 ?g loaded). Molecular weight marker (not shown) indicates a single band of the expected MW (43 kDa). The blot was incubated with a 1:500 dilution of the antibody at RT for 1 h followed by detection using IRDye800 labeled Goat-a-Rabbit IgG [H&L]  diluted 1:1,000. IRDye800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. Rabbit Aff - Purified Hu, Ms, Rt C, E, ICC/IF, IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Sialidase-2 (N-term) antibody

NEU2 Antibody (N-term) (Cat. #TA324482) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the NEU2 antibody detected the NEU2 protein (arrow). Rabbit Ig Aff - Purified Hu F, ICC/IF, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

Sialidase-2 antibody

Host: Rabbit; Target Name: NEU2; Sample Tissue: 293T Whole Cell lysates; Antibody Dilution: 1.0ug/ml Rabbit IgG Purified Can, Hu, Por WB
50 µg / €325.00
  OriGene Technologies, Inc.

Sialidase-2 antibody

Anti-NEU2 mouse monoclonal antibody (TA502914) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NEU2(RC219858 ). Mouse IgG1 OTI5E1 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Sialidase-2 antibody

Anti-NEU2 mouse monoclonal antibody (TA502942) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NEU2(RC219858 ). Mouse IgG2a OTI4F4 Purified Hu F, ICC/IF, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

Sialidase-2 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEU2 (RC219858, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEU2. Mouse IgG2b OTI8C12 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Sialidase-2 antibody

Sialidase-2 Mouse Serum Hu, Rt E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

Sialidase-2 antibody

Detection limit for recombinant GST tagged NEU2 is approximately 1ng/ml as a capture antibody. Mouse IgG2a 3G9 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

Sialidase-2 antibody

NEU2 monoclonal antibody (M03), clone 3B9. Western Blot analysis of NEU2 expression in human liver. Mouse IgG2a 3B9 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Sialidase-2 antibody

Detection limit for recombinant GST tagged NEU2 is approximately 3ng/ml as a capture antibody. Mouse IgG2a 2E5 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Sialidase-2

Sialidase-2 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

Sialidase-2 (full length, N-term HIS tag)

Sialidase-2 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

Sialidase-2

Sialidase-2 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

Sialidase-2

Sialidase-2 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

NEU2 overexpression lysate

NEU2 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

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