SIGLEC12 antibody

Principal name

SIGLEC12 antibody

Alternative names for SIGLEC12 antibody

Siglec-12, SIGLECL1, Siglec-L1, SLG, Sialic acid-binding Ig-like lectin 12, Sialic acid-binding Ig-like lectin-like 1

SwissProt ID

Q91Y57 (Mouse), Q96PQ1 (Human)

Gene ID

89858 (SIGLEC12)

Available reactivities

Chimp (Chimpanzee), Hu (Human), Bov (Bovine), Rt (Rat), Ms (Mouse), Eq (Equine), Por (Porcine), Ze (Zebrafish)

Available hosts

Goat, Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Paraffin Sections (P)

Background of SIGLEC12 antibody

Sialic acid-binding immunoglobulin-like lectins (SIGLECs) are a family of cell surface proteins belonging to the immunoglobulin superfamily. They mediate protein-carbohydrate interactions by selectively binding to different sialic acid moieties present on glycolipids and glycoproteins. SIGLEC12 is a member of the SIGLEC3-like subfamily of SIGLECs. SIGLEC12, upon tyrosine phosphorylation, has been shown to recruit the Src homology 2 domain-containing protein-tyrosine phosphatases SHP1 and SHP2. It has been suggested that the protein is involved in the negative regulation of macrophage signaling by functioning as an inhibitory receptor.Western blots using four different antibodies against four unique regions of this protein target confirm the same apparent molecular weight in our tests.Sialic acid-binding immunoglobulin-like lectins (SIGLECs) are a family of cell surface proteins belonging to the immunoglobulin superfamily. They mediate protein-carbohydrate interactions by selectively binding to different sialic acid moieties present on glycolipids and glycoproteins. This gene encodes a member of the SIGLEC3-like subfamily of SIGLECs. Members of this subfamily are characterized by an extracellular V-set immunoglobulin-like domain followed by two C2-set immunoglobulin-like domains, and the cytoplasmic tyrosine-based motifs ITIM and SLAM-like. The encoded protein, upon tyrosine phosphorylation, has been shown to recruit the Src homology 2 domain-containing protein-tyrosine phosphatases SHP1 and SHP2. It has been suggested that the protein is involved in the negative regulation of macrophage signaling by functioning as an inhibitory receptor. This gene is located in a cluster with other SIGLEC3-like genes on 19q13.4. Alternatively spliced transcript variants encoding distinct isoforms have been described for this gene.

General readings

Angata,T., (2001) J. Biol. Chem. 276 (43), 40282-40287

14 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SIGLEC12 antibody

  Rabbit Polyclonal Anti-SIGLEC12 Antibody  
WB Suggested Anti-SIGLEC12 Antibody Titration: 0.2-1 ug/ml; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Eq, Hu, Por, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

SIGLEC12 antibody

  Rabbit Polyclonal Anti-SIGLEC12 Antibody  
WB Suggested Anti-SIGLEC12 Antibody Titration: 0.2-1 ug/ml; Positive Control: Jurkat cell lysate Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

SIGLEC12 antibody

  Rabbit Polyclonal Anti-SIGLEC12 Antibody  
WB Suggested Anti-SIGLEC12 Antibody Titration: 0.2-1 ug/ml; Positive Control: HepG2 cell lysate Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

SIGLEC12 antibody

  Rabbit Polyclonal Anti-SIGLEC12 rabbit polyclonal antibody  
Gel: 8%SDS-PAGE,Lysate: 40 ug,,Primary antibody: (SIGLEC12 Antibody) at dilution 1/500 dilution,Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,Exposure time: 1 minute Rabbit IgG Purified Hu, Ms E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SIGLEC12 (C-term) antibody

Western blot analysis of SIGLEC12 Antibody (C-term) (AP18196PU-N) in mouse bladder tissue lysates (35ug/lane). SIGLEC12 (arrow) was detected using the purified Pab. Rabbit Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

SIGLEC12 - human gene knockout kit via CRISPR

SIGLEC12 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

SIGLEC12 - human gene knockout kit via CRISPR

SIGLEC12 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

SIGLEC12 - human gene knockout kit via CRISPR

SIGLEC12 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

SIGLEC12 - human gene knockout kit via CRISPR

SIGLEC12 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

SIGLEC12 (transcript variant 1)

  Recombinant protein of human sialic acid binding Ig-like lectin 12 (SIGLEC12), transcript variant 1  
SIGLEC12 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

SIGLEC12 overexpression lysate

SIGLEC12 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

SIGLEC12 overexpression lysate

SIGLEC12 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, SIGLEC12 (mGFP-tagged) - Human sialic acid binding Ig-like lectin 12 (SIGLEC12), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, SIGLEC12 (mGFP-tagged) - Human sialic acid binding Ig-like lectin 12 (SIGLEC12), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €1,100.00
  OriGene Technologies, Inc.

Lenti ORF particles, SIGLEC12 (Myc-DDK tagged) - Human sialic acid binding Ig-like lectin 12 (SIGLEC12), transcript variant 2 , 200 uL, >10^7 TU/mL

Lenti ORF particles, SIGLEC12 (Myc-DDK tagged) - Human sialic acid binding Ig-like lectin 12 (SIGLEC12), transcript variant 2 , 200 uL, >10^7 TU/mL  
200 µl / €980.00
  OriGene Technologies, Inc.

14 Item(s)

per page
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