SIK1 antibody

Principal name

SIK1 antibody

Alternative names for SIK1 antibody

SIK-1, SIK, SNF1LK, Serine/threonine-protein kinase SIK1, Salt-inducible protein kinase 1, Serine/threonine-protein kinase SNF1-like kinase 1

SwissProt ID

P57059 (Human), Q60670 (Mouse), Q9R1U5 (Rat)

Gene ID

102724428 (LOC102724428), 59329 (Sik1), 150094 (SIK1), 17691

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Dros (Drosophila), Ms (Mouse), Rt (Rat), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of SIK1 antibody

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.

General readings

Nishimura,Y., (1999) Biochem. Biophys. Res. Commun. 254 (1), 21-26

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SIK1 antibody

  Rabbit polyclonal SIK (Ab-182) antibody  
Western blot analysis of extracts from COLO cells, treated with PMA (125ng/ml, 30mins), using SIK (Ab-182) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SIK1 (Center) antibody

  Rabbit Polyclonal SIK1 Antibody  
Western blot analysis of SIK1 in human small intestine tissue lysate with SIK1 antibody at 1 ug/mL. Rabbit Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SIK1 antibody

  Rabbit Polyclonal Anti-SNF1LK Antibody  
WB Suggested Anti-SNF1LK Antibody Titration: 1.25 ug/ml; Positive Control: Jurkat cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SIK1 antibody

  Rabbit Polyclonal Anti-SIK1 Antibody  
Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane 1-5: HT29 cells, hela cells, mouse liver tissue, A549 cells, Jurkat cells, Primary antibody: (SIK1 Antibody) at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SIK1 (1-101) antibody

Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2a 2C12 Purified Hu E, P
50 µg / €530.00
  Acris Antibodies GmbH

SIK1 (C-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

SIK1 antibody

Immunofluorescence of purified MaxPab antibody to SNF1LK on HeLa cell. [antibody concentration 10 ug/ml] Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

SIK1 antibody

SIK1 MaxPab rabbit polyclonal antibody. Western Blot analysis of SIK1 expression in mouse lung. Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

SIK1 antibody

SIK1 Mouse IgG2a 2C12 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

SIK1 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with SNF1LK polyclonal antibody ( Cat # PAB3316 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma. Rabbit Hu E, P, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

SIK1 antibody

Western blot analysis of extracts from COLO205 cells, treated with PMA 125ng/ml 30', using SIK (Ab-182) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Rt E, WB
50 µg / €230.00
  Assay Biotech

SIK1 antibody

Western blot analysis of extracts from COLO205 cells, treated with PMA 125ng/ml 30', using SIK (Ab-182) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Rt E, WB
0.1 mg / €330.00
  Assay Biotech

SIK1 (Center) antibody

Rat Lung; WB Suggested Anti-Sik1 Antibody. . Titration: 1.0 ug/ml. . Positive Control: Rat Lung; Sik1 antibody - middle region (ARP45917_P050) in Rat Lung cells using Western Blot Rabbit Aff - Purified Hu, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

SIK1 antibody

Human Stomach; SNF1LK antibody - N-terminal region (ARP45918_T100) in Human Stomach cells using Immunohistochemistry Rabbit Aff - Purified Bov, Can, Chk, Dros, Hu, Ms, Rt P, WB
0.1 mg / €310.00
  AVIVA Systems Biology

CRISPR

Catalog No. Species Donor Vector  

SIK1 - human gene knockout kit via CRISPR

SIK1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

SIK1 - human gene knockout kit via CRISPR

SIK1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

SIK1 - human gene knockout kit via CRISPR

SIK1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

SIK1 - human gene knockout kit via CRISPR

SIK1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Sik1 - mouse gene knockout kit via CRISPR

Sik1 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Sik1 - mouse gene knockout kit via CRISPR

Sik1 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

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