SIN3A antibody

Principal name

SIN3A antibody

Alternative names for SIN3A antibody

Paired amphipathic helix protein Sin3a, Transcriptional corepressor Sin3a, Histone deacetylase complex subunit Sin3a

SwissProt ID

Q60520 (Mouse), Q96ST3 (Human)

Gene ID

20466 (Sin3a), 25942 (SIN3A)

Available reactivities

Hu (Human), Ms (Mouse), Chk (Chicken), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), PLA/Proximity ligation assay (PLA), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), ChIP assay (ChIP)

Background of SIN3A antibody

SIN3 was origilly identified as a negative regulator of transcription in budding yeast. Since then, three isoforms of the SIN3 proteins have been identified in mammalian cells, as products of two different genes, SIN3A and SIN3B. Both SIN3A and SIN3B are nuclear proteins that function as scaffolding subunits for the multi-subunit SIN3 transcriptiol repressor complex, containing SIN3A or SIN3B, HDAC1, HDAC2, SDS3, RBBP4/RBAP48, RBBP7/RBAP46, SAP30, and SAP18. SIN3 proteins contain four paired amphipathic alpha-helix (PAH) motifs that function in the recruitment of the SIN3 complex to target genes by binding a multitude of D-binding transcriptiol repressor proteins, including Mad1, p53, E2F4, HCF-1, AML1, Elk-1, NRSF, CTCF, ERa, and MeCP2. In addition, SIN3 proteins contain an HDAC interaction domain (HID), which mediates binding of HDAC1 and HDAC2 via the SDS3 bridging protein, and a highly conserved region (HCR) at the carboxy terminus, which contributes to repressor protein binding. RBBP4 and RBBP7 proteins also bind to SDS3 and contribute to nucleosome binding of the complex. The SIN3 complex functions to repress transcription, in part, by deacetylating histones at target gene promoters. In addition, recent studies have shown that SIN3 is recruited to the coding regions of repressed and active genes, where it deacetylates histones and suppresses spurious transcription by R polymerase II. In addition to histone deacetylase activity, the SIN3 complex associates with histone methyltransferase (ESET), histone demethylase (JARID1A/RBP2), ATP-dependent chromatin remodeling (SWI/SNF), methylcytosine dioxygese (TET1), and O-Glcc transferase (OGT) activities, all of which appear to contribute to the regulation of target genes. The SIN3 complex is critical for proper regulation of embryonic development, cell growth and proliferation, apoptosis, D replication, D repair, and D methylation (imprinting and X-chromosome ictivation)

General readings

General / background references:Razin A CpG methylation, chromatin structure and gene silencing-a three-way connection. EMBO J 17:4905-8 (1998). PubMed PMID: 9724627
Wong CW & Privalsky ML Transcriptional repression by the SMRT-mSin3 corepressor: multiple interactions, multiple mechanisms, and a potential role for TFIIB. Mol Cell Biol 18:5500-10 (1998). PubMed PMID: 9710634
Ayer DE et al. Mad-Max transcriptional repression is mediated by ternary complex formation with mammalian homologs of yeast repressor Sin3. Cell 80:767-76 (1995). PubMed PMID: 7889570

13 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SIN3A antibody

Western blot analysis of extracts of various cell lines, using SIN3A antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

SIN3A antibody

WB Suggested Anti-SIN3A Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Hela cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

SIN3A antibody

SIN3A Mouse IgG1 1B7 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SIN3A antibody

Human Placenta; Immunohistochemistry with Human Placenta lysate tissue at an antibody concentration of 5.0ug/ml using anti-SIN3A antibody (ARP32483_P050); SIN3A antibody - N-terminal region (ARP32483_P050) in Human Placenta cells using Immunohistochemistry Rabbit Aff - Purified Chk, Hu, Ms, Rt P, WB
50 µg / €350.00
  AVIVA Systems Biology

SIN3A antibody

Immunohistochemistry-Paraffin: Sin3A Antibody [NB100-2252] - See Notes, IHC Caption A Rabbit Aff - Purified Hu, Ms IP, P, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

SIN3A antibody

WB and IP: Sin3A Antibody [NB100-2253] - Detection of Human and Mouse Sin3A on HeLa whole cell lysate using NB100-2253. Sin3A was  also immunoprecipitated using rabbit anti-Sin3A antibodies NB100-2252, NB100-2254 and another Sin3A antibody. Rabbit Aff - Purified Hu, Ms IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

SIN3A antibody

Western Blot: Sin3A Antibody [NB100-2254] - Detection of Human Sin3A by Western Blot of  Immunoprecipitates.  Samples:  Whole cell lysate (1 mg for IP,  20% of IP loaded) from HeLa cells.  Antibodies:  Affinity purified  rabbit anti-Sin3A antibody NB100-2254 used  for IP at 3 mcg/mg lysate. Sin3A was also immunoprecipitated  using rabbit anti-Sin3A antibodies NB100-2252, NB100-2253 and another Sin3A antibody at 3  mcg/mg lysate.  Detection:  Chemiluminescence with exposure times of 3 seconds  (A) and 10 seconds (B). Rabbit Aff - Purified Hu IP, PLA
0.1 mg / €440.00
  Novus Biologicals Inc.

SIN3A antibody

Western Blot: mSin 3A Antibody [NB600-1263] - Figure 1 shows a Western blot of mSin3A on K562 cell extract using NB600-1263. Rabbit IgG Aff - Purified Hu, Ms IP, WB
0.1 mg / €370.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

SIN3A

SIN3A Human
2 µg / €300.00
  Abnova Taiwan Corp.

SIN3A

SIN3A Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

SIN3A

SIN3A Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

SIN3A

SIN3A Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

SIN3A

SIN3A Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

13 Item(s)

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