SIPA1 / SPA1 antibody

Principal name

SIPA1 / SPA1 antibody

Alternative names for SIPA1 / SPA1 antibody

Sipa-1, Spa-1, p130 SPA-1, Signal-induced proliferation-associated protein 1, GTPase-activating protein Spa-1

SwissProt ID

P46062 (Mouse), Q96FS4 (Human)

Gene ID

6494 (SIPA1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Hst (Hamster)

Available hosts

Rabbit, Mouse, Goat

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of SIPA1 / SPA1 antibody

Kit Component:
- KN203747G1, SIPA1 gRNA vector 1 in pCas-Guide vector
- KN203747G2, SIPA1 gRNA vector 2 in pCas-Guide vector
- KN203747D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

Hattori M, Tsukamoto N, Nur-e-Kamal MS, Rubinfeld B, Iwai K, Kubota H, Maruta H, Minato N.
Molecular cloning of a novel mitogen-inducible nuclear protein with a Ran GTPase-activating domain that affects cell cycle progression.
Mol Cell Biol. 1995 Jan;15(1):552-60.
PMID: 7799964

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SIPA1 / SPA1 (C-term) antibody

  Goat Polyclonal Antibody against SIPA1  
TA302921 staining (1µg/ml) of Human EBV-immortilized lymphoblastoid lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SIPA1 / SPA1 antibody

  Rabbit polyclonal anti-Sipa1 antibody  
WB using Anti-Sipa1 antibody shows detection of over-expressed Sipa1 in lysates from mouse 3T3 cells transfected with Sipa1 (lane 1). Endogenous Sipa1 is detected in lane 2, which contains lysate from 3T3 cells mock-transfected with LacZGLB. Lane 3 and 4 are similar to lanes 1 and 2 except the antibody was preincubated with the immunizing peptide prior to reaction with the membrane. Primary antibody was used at 1:1250. Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SIPA1 / SPA1 (N-term) antibody

  Rabbit Polyclonal SIPA1 Antibody  
Western blot analysis of SIPA1 in human brain tissue lysate with SIPA1 antibody at 1 ug/mL. Rabbit IgG Aff - Purified Hu E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SIPA1 / SPA1 antibody

  Anti-SIPA1 Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm,Nucleus. Positive control: Human liver and lung cancer tissue . Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human liver cancer tissue using SIPA1 antibody at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

SIPA1 / SPA1 antibody

  Anti-SIPA1 Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm. Positive control: Human colon and cervical cancer tissue . Recommended dilution: 1/50-200  The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using SIPA1 antibody at dilution 1/40, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

SIPA1 / SPA1 antibody

  Rabbit Polyclonal Anti-SIPA1 Antibody - middle region  
WB Suggested Anti-SIPA1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: HT1080 cell lysate Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

SIPA1 / SPA1 antibody

  Rabbit Polyclonal SIPA1 Antibody  
Immunohistochemical staining of human rectum shows strong nuclear and cytoplasmic positivity in glandular cells.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only. Rabbit IgG Purified Hu E, ICC/IF, P
0.1 mg / €325.00
  OriGene Technologies, Inc.

SIPA1 / SPA1 antibody

Western blot analysis of human SPA-1 expression in Raji (1), HL-60 (2), Jurkat (3), HeLa (4) and 293T (5) using AM26544AF-N. Mouse IgG1 331-9 Azide Free Hu IP, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

SIPA1 / SPA1 antibody

Western blot analysis of mouse SPA-1 expression in NIH/3T3 (1), WR19L (2), CHO (3) and MEF (4) using AM26545AF-N. Mouse IgG2a 2-22-9 Azide Free Hst, Ms IP, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

SIPA1 / SPA1 antibody

SIPA1 / SPA1 Mouse IgG 2B4 Purified Hu WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SIPA1 / SPA1 antibody

SIPA1 / SPA1 Mouse IgG1 2B4 Ascites Hu E
0.2 ml / €370.00
  Abnova Taiwan Corp.

SIPA1 / SPA1 antibody

SIPA1 polyclonal antibody ( Cat # PAB6576 ) staining ( 1 µg/ml ) of human EBV-immortilized lymphoblastoid lysate ( RIPA buffer, 35 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

SIPA1 / SPA1 antibody

Immunohistochemistry of Sipa1 polyclonal antibody ( Cat # PAB11325 ) was used at 1.25 µg/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides.
This image shows moderate to strong positive staining of lymphocytes within human tonsil at 40X.
Tissue was formalin-fixed and paraffin embedded.
The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
Personal Communication, Tina Roush, Life Span Biosciences, Seattle, WA. Rabbit Hu, Ms, Rt E, P, WB
0.1 mg / €560.00
  Abnova Taiwan Corp.

SIPA1 / SPA1 antibody

Human HT1080; WB Suggested Anti-SIPA1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: HT1080 cell lysate; SIPA1 antibody - middle region (ARP58780_P050) in Human HT1080 cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

CRISPR

Catalog No. Species Donor Vector  

SIPA1 - human gene knockout kit via CRISPR

SIPA1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

SIPA1 - human gene knockout kit via CRISPR

SIPA1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

SIPA1 - human gene knockout kit via CRISPR

SIPA1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

SIPA1 - human gene knockout kit via CRISPR

SIPA1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

SIPA1 / SPA1

SIPA1 / SPA1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

SIPA1 / SPA1

SIPA1 / SPA1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

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