SIPA1 / SPA1 antibody

Principal name

SIPA1 / SPA1 antibody

Alternative names for SIPA1 / SPA1 antibody

Sipa-1, Spa-1, p130 SPA-1, Signal-induced proliferation-associated protein 1, GTPase-activating protein Spa-1

SwissProt ID

P46062 (Mouse), Q96FS4 (Human)

Gene ID

6494 (SIPA1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Hst (Hamster)

Available hosts

Mouse, Goat, Rabbit

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of SIPA1 / SPA1 antibody

**Please note, this product is one of a range of Investigative Grade antibodies, made against targets that have limited or no commercial antibodies available to them and for which there are no data on the expression of the protein in the range of common cell lines and tissues available to us. These antibodies are affinity purified using their peptide immunogen and are known to give low background staining in a western blot. However no additional claims are made for their ability to recognise native protein in any application. SIPA1 is a mitogen induced GTPase activating protein (GAP). It exhibits a specific GAP activity for Ras-related regulatory proteins Rap1 and Rap2, but not for Ran or other small GTPases. This protein may also hamper mitogen-induced cell cycle progression when abnormally or prematurely expressed. It is localized to the perinuclear region.

General readings

Hattori M, Tsukamoto N, Nur-e-Kamal MS, Rubinfeld B, Iwai K, Kubota H, Maruta H, Minato N.
Molecular cloning of a novel mitogen-inducible nuclear protein with a Ran GTPase-activating domain that affects cell cycle progression.
Mol Cell Biol. 1995 Jan;15(1):552-60.
PMID: 7799964

20 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SIPA1 / SPA1 (C-term) antibody

TA302921 staining (1µg/ml) of Human EBV-immortilized lymphoblastoid lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SIPA1 / SPA1 antibody

WB using Anti-Sipa1 antibody shows detection of over-expressed Sipa1 in lysates from mouse 3T3 cells transfected with Sipa1 (lane 1). Endogenous Sipa1 is detected in lane 2, which contains lysate from 3T3 cells mock-transfected with LacZGLB. Lane 3 and 4 are similar to lanes 1 and 2 except the antibody was preincubated with the immunizing peptide prior to reaction with the membrane. Primary antibody was used at 1:1250. Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SIPA1 / SPA1 (N-term) antibody

Western blot analysis of SIPA1 in human brain tissue lysate with SIPA1 antibody at 1 ug/mL. Rabbit IgG Aff - Purified Hu E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SIPA1 / SPA1 antibody

Predicted cell location: Cytoplasm,Nucleus. Positive control: Human liver and lung cancer tissue . Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human liver cancer tissue using SIPA1 antibody at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

SIPA1 / SPA1 antibody

Predicted cell location: Cytoplasm. Positive control: Human colon and cervical cancer tissue . Recommended dilution: 1/50-200  The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using SIPA1 antibody at dilution 1/40, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

SIPA1 / SPA1 antibody

WB Suggested Anti-SIPA1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: HT1080 cell lysate Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

SIPA1 / SPA1 antibody

Immunohistochemical staining of human rectum shows strong nuclear and cytoplasmic positivity in glandular cells.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only. Rabbit IgG Purified Hu E, ICC/IF, P
0.1 mg / €325.00
  OriGene Technologies, Inc.

SIPA1 / SPA1 antibody

Western blot analysis of human SPA-1 expression in Raji (1), HL-60 (2), Jurkat (3), HeLa (4) and 293T (5) using AM26544AF-N. Mouse IgG1 331-9 Azide Free Hu IP, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

SIPA1 / SPA1 antibody

Western blot analysis of mouse SPA-1 expression in NIH/3T3 (1), WR19L (2), CHO (3) and MEF (4) using AM26545AF-N. Mouse IgG2a 2-22-9 Azide Free Hst, Ms IP, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

SIPA1 / SPA1 (N-term) Control Peptide

SIPA1 / SPA1
50 µg / €160.00
  Acris Antibodies GmbH

SIPA1 / SPA1 antibody

SIPA1 / SPA1 Mouse IgG 2B4 Purified Hu WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SIPA1 / SPA1 antibody

SIPA1 / SPA1 Mouse IgG1 2B4 Ascites Hu E
0.2 ml / €370.00
  Abnova Taiwan Corp.

SIPA1 / SPA1 antibody

SIPA1 polyclonal antibody ( Cat # PAB6576 ) staining ( 1 µg/ml ) of human EBV-immortilized lymphoblastoid lysate ( RIPA buffer, 35 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

SIPA1 / SPA1 antibody

Immunohistochemistry of Sipa1 polyclonal antibody ( Cat # PAB11325 ) was used at 1.25 µg/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides.
This image shows moderate to strong positive staining of lymphocytes within human tonsil at 40X.
Tissue was formalin-fixed and paraffin embedded.
The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
Personal Communication, Tina Roush, Life Span Biosciences, Seattle, WA. Rabbit Hu, Ms, Rt E, P, WB
0.1 mg / €560.00
  Abnova Taiwan Corp.

SIPA1 / SPA1 antibody

Human HT1080; WB Suggested Anti-SIPA1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: HT1080 cell lysate; SIPA1 antibody - middle region (ARP58780_P050) in Human HT1080 cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

SIPA1 / SPA1 antibody

ELISA: SIPA1 Antibody [30850002] - Affinity Purified Rabbit Hu E, P
50 µg / €440.00
  Novus Biologicals Inc.

SIPA1 / SPA1 antibody

SIPA1 / SPA1 Goat IgG Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

SIPA1 / SPA1 antibody

Western Blot and Immunoprecipitation: SIPA1 Antibody [NBP1-18917] - Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells. SIPA1 was also immunoprecipitated by another rabbit anti-SIPA1 antibody. Rabbit IgG Aff - Purified Hu, Ms IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

SIPA1 / SPA1

SIPA1 / SPA1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

SIPA1 / SPA1

SIPA1 / SPA1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

20 Item(s)

per page
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