SIRT1 antibody

Principal name

SIRT1 antibody

Alternative names for SIRT1 antibody

SIRT-1, SIR2-like protein 1, SIR2L1, sirtuin type 1, sirtuin 1, sirtuin-1, SIR2alpha, sirtuin (silent mating type information regulation 2, S. cerevisiae, homolog) 1, NAD-dependent deacetylase sirtuin-1

SwissProt ID

Q923E4 (Mouse), Q96EB6 (Human)

Gene ID

23411 (SIRT1), 64383 (Sirt2)

Available reactivities

Hu (Human), Can (Canine), Ms (Mouse), Rt (Rat), Chk (Chicken), Por (Porcine), African clawed frog, Bov (Bovine), Fe (Feline), Mky (Monkey), Prim (Primate)

Available hosts

Rabbit, Mouse, Goat, Chicken

Available applications

ChIP assay (ChIP), Immunoprecipitation (IP), Western blot / Immunoblot (WB), PLA/Proximity ligation assay (PLA), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Flow Cytometry (F), Frozen Sections (C)

Background of SIRT1 antibody

D-dependent protein deacetylase that links transcriptiol regulation directly to intracellular energetics and participates in the coordition of several separated cellular functions such as cell cycle, response to D damage, metobolism, apoptosis and autophagy. Can modulate chromatin function through deacetylation of histones and can promote alterations in the methylation of histones and D, leading to transcriptiol repression. Deacetylates a broad range of transcription factors and coregulators, thereby regulating target gene expression positively and negatively. Serves as a sensor of the cytosolic ratio of D(+)/DH which is altered by glucose deprivation and metabolic changes associated with caloric restriction. Is essential in skeletal muscle cell differentiation and in response to low nutrients mediates the inhibitory effect on skeletal myoblast differentiation which also involves 5'-AMP-activated protein kise (AMPK) and nicotimide phosphoribosyltransferase (MPT). Component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rD in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of D(+)/DP(+) ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rD locus. Deacetylates 'Lys-266' of SUV39H1, leading to its activation. Inhibits skeletal muscle differentiation by deacetylating PCAF and MYOD1. Deacetylates H2A and 'Lys-26' of HIST1H1E. Deacetylates 'Lys-16' of histone H4 (in vitro). Involved in NR0B2/SHP corepression function through chromatin remodeling: Recruited to LRH1 target gene promoters by NR0B2/SHP thereby stimulating histone H3 and H4 deacetylation leading to transcriptiol repression. Proposed to contribute to genomic integrity via positive regulation of telomere length; however, reports on localization to pericentromeric heterochromatin are conflicting. Proposed to play a role in constitutive heterochromatin (CH) formation and/or maintence through regulation of the available pool of nuclear SUV39H1. Upon oxidative/metabolic stress decreases SUV39H1 degradation by inhibiting SUV39H1 polyubiquitition by MDM2. This increase in SUV39H1 levels enhances SUV39H1 turnover in CH, which in turn seems to accelerate renewal of the heterochromatin which correlates with greater genomic integrity during stress response. Deacetylates 'Lys-382' of p53/TP53 and impairs its ability to induce transcription-dependent proapoptotic program and modulate cell senescence. Deacetylates TAF1B and thereby represses rD transcription by the R polymerase I. Deacetylates MYC, promotes the association of MYC with MAX and decreases MYC stability leading to compromised transformatiol capability. Deacetylates FOXO3 in response to oxidative stre

General readings

Sun C. et al. SIRT1 Improves Insulin Sensitivity under Insulin-Resistant Conditions by repressing PTP1B. Cell Metabolism 6: 307-319, 2007.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SIRT1 antibody

Western blot analysis of extracts of various cell lines, using SIRT1 antibody. Rabbit IgG Purified Hu, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

SIRT1 antibody

Western Blot: SIRT1 Antibody (1F3) [TA336801] - Western blot analysis using SIRT1 mouse mAb against MCF-7 (1), Jurkat (2), Hela (3), HEK293 (4) and A549 (5) cell lysates. Mouse IgG1 1F3 Purified Hu, Ms, Prim, Rt C, E, F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SIRT1 antibody

Immunohistochemical analysis of paraffin-embedded lung cancer tissues (left) and kidney cancer tissues (right) using SIRT1 antibody Cat.-No AM06495SU-N with DAB staining. Mouse IgG1 1F3 Ascites Hu, Mky E, F, ICC/IF, P, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

SIRT1 (456-555) antibody

Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG1 7B7 Hu E, ICC/IF, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

SIRT1 antibody

Immunoperoxidase of monoclonal antibody to SIRT1 on formalin-fixed paraffin-embedded human lateral ventricle wall. [antibody concentration 1 ug/ml] Mouse IgG1 7B7 Purified Hu E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SIRT1 (577-590) antibody

TA303141 (0.1µg/ml) staining of HeLa cell lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SIRT1 (577-590) antibody

(0.05ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu, Ms E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SIRT1 (N-term) antibody

Western blot analysis of SIRT1 in mouse liver tissue lysate with SIRT1 antibody at 1 ug/mL. Chicken Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SIRT1 antibody

Western blot analysis of SirT1 expression in K562 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SIRT1 antibody

Western blot analysis of lysates from A549, 293 cell line (from left to right), using RPS6KB2 Antibody (Cat. #TA328121). TA328121 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35ug per lane. Mouse IgG3 1089CT5.3.1 Purified Hu WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

SIRT1 antibody

Western Blot: SIRT1 Antibody [TA336790] - Analysis of SIRT1 in HeLa whole cell lyates. Rabbit Purified Hu C, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SIRT1 antibody

Western blot detection of SIRT1 in Hela,Jurkat,Ramos,A549 and CEM cell lysates using SIRT1 mouse mAb (1:1000 diluted).Predicted band size:120KDa.Observed band size:120KDa. Mouse IgG2b 2G10-F4-D9 Purified WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SIRT1 antibody

WB was performed using nuclear extracts from HeLa cells (HeLa NE, 20 ug) and the antibody against hSirT1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left, the position of the protein of interest is shown on the right. Mouse IgG1 Monoclol Purified Hu
0.1 ml / €325.00
  OriGene Technologies, Inc.

SIRT1 antibody

WB was performed using nuclear extracts from HeLa cells (HeLa NE, 20 ug) and the antibody against hSirT1 diluted 1:500 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left), the position of the protein of interest is shown on the right. Mouse IgG1 Monoclol Purified Hu
50 µg / €325.00
  OriGene Technologies, Inc.

SIRT1 (C-term) antibody

Testis: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu IP, P, WB
50 µl / €470.00
  Acris Antibodies GmbH

SIRT1 control peptide

SIRT1
0.1 mg / €250.00
  Acris Antibodies GmbH

SIRT1 (33-55) antibody

SIRT1 Rabbit IgG Aff - Purified Bov, Chk, Fe, Hu, Ms, Rt WB
0.1 mg / €420.00
  Acris Antibodies GmbH

SIRT1 control peptide

SIRT1
50 µg / €160.00
  Acris Antibodies GmbH

SIRT1 pSer47 antibody

Western blot analysis of extracts from JK tissue using SirT1 (Phospho-Ser47) antibody AP55882PU-N.The lane on the right is treated with the antigen-specific peptide. Rabbit Aff - Purified Hu, Ms WB
0.1 ml / €340.00
  Acris Antibodies GmbH

SIRT1 pSer47 antibody

Western blot analysis of extracts from JK tissue using SirT1 (Phospho-Ser47) antibody AP55882PU-N.The lane on the right is treated with the antigen-specific peptide. Rabbit Aff - Purified Hu, Ms WB
50 µl / €270.00
  Acris Antibodies GmbH

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