SIRT2 / SIR2 antibody

Principal name

SIRT2 / SIR2 antibody

Alternative names for SIRT2 / SIR2 antibody

SIRT-2, SIR2-like protein 2, SIR2L, SIR2L2, sirtuin type 2, sirtuin 2, sirtuin-2, sirtuin (silent mating type information regulation 2 homolog) 2 (S. cerevisiae), NAD-dependent deacetylase sirtuin-2

SwissProt ID

Q5RJQ4 (Rat), Q7ZVK3 (Danre), Q8IXJ6 (Human), Q8VDQ8 (Mouse)

Gene ID

22933 (SIRT2), 361532 (Sirt2)

Available reactivities

Bov (Bovine), Chk (Chicken), Fe (Feline), Hu (Human), Ms (Mouse), Rt (Rat), Por (Porcine), Ze (Zebrafish), Mky (Monkey), Ce (C. elegans)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Flow Cytometry (F)

Background of SIRT2 / SIR2 antibody

Sirtuins are constitutively expressed in all domains of life. It was first identified in Saccharamyces cerevisiae as sirtuin, Sir2 (silent information regulator 2) which regulates ribosomal DNA recombination, gene silencing, DNA repair, chromosomal stability and longevity. The activities of epigenetic regulators such as histone acetyltransferases (HDACs), methyltransferases, chromatin-remodeling enzymes, etc., play a fundamental role in the control of gene expression by covalently modifying the local state of chromatin. The science of epigenetic regulation is a fairly new in gene regulation and not much is known about the regulation of these epigenetic-regulators by alternative splicing. The chromatin modulations is achieved by a series of chromatin modifying enzymes by either introducing histone modifications or by inducing ATP-dependent nucleosome remodeling. The regulatory state of a gene is explained by extend of covalent modifications (acetylation, methylation and ATP-dependent phosphorylation of histones as explained by “histone code hypothesis”.
The cytoplasmic sirtuin 2 is the most abundant sirtuin family member expressed in adipocytes, its expression is down regulated in 3T3 cells during their transformation in to adipocytes, the process of morphological differentiation is inhibited by over-expression of sirtuin 2 (2). In 3T3 cells the situin 2 controls the acetylation of Fox 1 protein (1). The adiopocyte differentiation is modulated by sirtuin 2 via acetylation of Fox1 protein. The microtubule regulatory alpha-tublin deactylases, histone deacetylase-6 (HDAC6) and Sirtuin 2 are the major players in cellular development of nervous system where all major cell types undergo cellular differentiation, generate unsurpassed quantities of plasma membranes and extend cellular processes to communicate and regulate the activities of other cells. The HDAC6 is abundantly expressed in Purkinje cells in hippocampus and Sirtuin 2 is mainly associated with myelin sheaths. The expression of these proteins in post-mitotic oligodendrocytes suggests their novel function in process out-growth and membrane remodeling by Sir2 in conjunction with the microtubule destabilizing protein Stathmin 1 during the early myelognesis. The lack of Sir 2 in oligodandrocytes of spastic paraplegic mouse model deficient in proteolipid protein (PLP)/DM20 suggest that lack of Sir 2 expression is post-translational and is mainly due to absence of transport carrier P:P/DM20 (3).

General readings

1. Yamamoto H, Schoonjans K, and Auwerx J. Sirtuin functions in health and disease. Mol. Endocrinol. 2007; 21:1745-55.
2. Frye RA. Characterization of five human cDNAs with homology to the yeast SIR2 gene: SIR2-like proteins (sirtuins) metabolize NAD and may have protein ADP-ribosyltransferase activity. Biochem. Biophys. Res. Commun. 1999; 260:273-279.
3. North BJ, Marshall BL, Borra MT, et. al. The human Sir2 ortholog, SIRT2, is an NAD+-dependent tubulin deacetylase. Mol. Cell 2003; 11:437-44.
4. Inoue T, Hiratsuka M, Osaki M, et al. The molecular biology of mammalian SIRT proteins: SIRT2 in cell cycle regulation. Cell Cycle 2007; 6:1011-8.
5. Outeiro TF, Kontopoulos E, Altmann SM, et al. Sirtuin 2
Inhibitors Rescue -Synuclein-Mediated Toxicity in Models of Parkinson's Disease. Science 2007; 317:516–519.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SIRT2 / SIR2 (C-term) antibody

Human Placenta (formalin-fixed, paraffin-embedded) stained with SIRT2 antibody AP22673PU-N at 5 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit Purified Hu, Ms, Rt E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

SIRT2 / SIR2 antibody

Immunofluorescence of monoclonal antibody to SIRT2 on HeLa cell . [antibody concentration 10 ug/ml] Mouse IgG1 4B11 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SIRT2 / SIR2 antibody

Immunohistochemistry-Paraffin: SIRT2 Antibody [NBP1-50461] - Human Heart (formalin-fixed, paraffin-embedded) stained with SIRT2 antibody at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €480.00
  Novus Biologicals Inc.

SIRT2 / SIR2 (C-term) antibody

Western blot analysis of SIRT2 in Mouse brain lysate with SIRT2 antibody at (A) 1 and (B) 2 ug/ml. Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SIRT2 / SIR2 (N-term) antibody

Western blot analysis of SIRT2 in human brain lysate with SIRT2 antibody at (A) 2.5 and (B) 5 ug/ml. Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SIRT2 / SIR2 antibody

Western blot analysis of extracts from 293 cells and NIH/3T3 cells, using SIRT2 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SIRT2 / SIR2 antibody

Predicted band size: 43 kDa . Positive control: Human fetal brain tissue lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/100 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 minutes) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SIRT2 / SIR2 antibody

Predicted band size: 43 kDa . Positive control: Mouse heart tissue lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/350 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 30 seconds) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SIRT2 / SIR2 antibody

Western blot analysis of extracts of mouse brain tissue, using SIRT2 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SIRT2 / SIR2 antibody

Western Blot: Sir2.1 Antibody [TA336275] - Western blot using TA336275 against His tagged SIR-2.1 full-length protein. Rabbit Purified Ce C, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SIRT2 / SIR2 antibody

Western blot analysis of SIRT2 expression in 293 cells Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

SIRT2 / SIR2 control peptide

SIRT2 / SIR2
0.1 mg / €250.00
  Acris Antibodies GmbH

SIRT2 / SIR2 (308-324) antibody

Western blot using Sirtuin-2 antibody (AP10323PU-N at 1/500 dilution) Rabbit IgG Aff - Purified Bov, Chk, Fe, Hu, Ms, Rt WB
0.1 mg / €420.00
  Acris Antibodies GmbH

SIRT2 / SIR2 (N-term) antibody

Human Brain, Cerebellum (formalin-fixed, paraffin-embedded) stained with SIRT2 antibody AP22674PU-N at 5 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit Purified Hu, Ms, Rt E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

SIRT2 / SIR2 Control Peptide

SIRT2 / SIR2
50 µg / €160.00
  Acris Antibodies GmbH

SIRT2 / SIR2 Control Peptide

SIRT2 / SIR2
50 µg / €160.00
  Acris Antibodies GmbH

SIRT2 / SIR2 antibody

Western Blot analysis of SIRT2 expression in transfected 293T cell line (H00022933-T01) by SIRT2 MaxPab polyclonal antibody.

Lane 1: SIRT2 transfected lysate(38.72 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

SIRT2 / SIR2 antibody

Western Blot analysis of SIRT2 expression in transfected 293T cell line (H00022933-T02) by SIRT2 MaxPab polyclonal antibody.

Lane 1: SIRT2 transfected lysate(38.72 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

SIRT2 / SIR2 antibody

Western Blot analysis of SIRT2 expression in transfected 293T cell line (H00022933-T02) by SIRT2 MaxPab polyclonal antibody.

Lane 1: SIRT2 transfected lysate(39.50 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

SIRT2 / SIR2 antibody

SIRT2 / SIR2 Rabbit Hu, Ms, Rt WB
0.1 mg / €470.00
  Abnova Taiwan Corp.

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