SIRT3 antibody

Principal name

SIRT3 antibody

Alternative names for SIRT3 antibody

SIRT-3, hSIRT3, SIR2-like protein 3, SIR2L3, sirtuin type 3, sirtuin 3, sirtuin-3, sirtuin (silent mating type information regulation 2 homolog) 3 (S. cerevisiae), NAD-dependent deacetylase sirtuin-3 mitochondrial

SwissProt ID

Q8R104 (Mouse), Q9NTG7 (Human)

Gene ID

23410 (SIRT3), 64384 (Sirt3)

Available reactivities

Can (Canine), Hu (Human), Ms (Mouse), Rb (Rabbit), Rt (Rat), Dros (Drosophila), Ze (Zebrafish), African clawed frog, Bov (Bovine), Chk (Chicken), Fe (Feline)

Available hosts

Rabbit, Mouse, Goat, Chicken

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Enzyme Immunoassay (E)

Background of SIRT3 antibody

Sirtuins are constitutively expressed in all domains of life. It was first identified in Saccharamyces cerevisiae as sirtuin, Sir2 (silent information regulator 2) which regulates ribosomal DNA recombination, gene silencing, DNA repair, chromosomal stability and longevity. The activities of epigenetic regulators such as histone acetyltransferases (HDACs), methyltransferases, chromatin-remodeling enzymes, etc., play a fundamental role in the control of gene expression by covalently modifying the local state of chromatin. The science of epigenetic regulation is a fairly new in gene regulation and not much is known about the regulation of these epigenetic-regulators by alternative splicing. The chromatin modulations is achieved by a series of chromatin modifying enzymes by either introducing histone modifications or by inducing ATP-dependent nucleosome remodeling. The regulatory state of a gene is explained by extend of covalent modifications (acetylation, methylation and ATP-dependent phosphorylation of histones as explained by “histone code hypothesis”.
There are several mammalian homolog of yeast protein Sirtuin 2 (Sir 2) including Sirtuin 3, Sirtuin 4 and Sirtuin 5. The yeast homologs are involved in extension of yeast longevity by caloric restriction. The localization of sirtuin 3, 4 and 5 are localized in distinct compartment of mitochondrion. When Sir 3 and 5 are coexpressed the localizxation of Sir 3 changed from mitochondria to nucleus (1). The cytoplasmic sirtuin 2 is the most abundant sirtuin family member expressed in adipocytes, its expression is down regulated in 3T3 cells during their transformation in to adipocytes, the process of morphological differentiation is inhibited by over-expression of sirtuin 2 (2). In 3T3 cells the sirtuin 2 controls the acetylation of Fox 1 protein (1). The adiopocyte differentiation is modulated by sirtuin 2 via acetylation of Fox1 protein. The microtubule regulatory alpha-tublin deactylases, histone deacetylase-6 (HDAC6) and Sirtuin 2 are the major players in cellular development of nervous system where all major cell types undergo cellular differentiation, generate unsurpassed quantities of plasma membranes and extend cellular processes to communicate and regulate the activities of other cells. The HDAC6 is abundantly expressed in Purkinje cells in hippocampus and Sirtuin 2 is mainly associated with myelin sheaths.
The expression of these proteins in post-mitotic oligodendrocytes suggests their novel function in process out-growth and membrane remodeling by Sir2 in conjunction with the microtubule destabilizing protein Stathmin 1 during the early myelognesis. The lack of Sir 2 in oligodandrocytes of spastic paraplegic mouse model deficient in proteolipid protein (PLP)/DM20 suggest that lack of Sir 2 expression is post-translational and is mainly due to absence of transport carrier P:P/DM20 (3).

General readings

Shi T, Wang F, Stieren E, Tong Q. SIRT3, a mitochondrial Sirtuin deacetylase, regulates mitochondrial function and thermogenesis in Brown adipocytes. J Biol Chem. 2005 Jan 14

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SIRT3 (C-term) antibody

  Goat Polyclonal Antibody against SIRT3  
TA302609 (0.2µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SIRT3 (Center) antibody

  Chicken Polyclonal SIRT3 Antibody  
Western blot analysis of SIRT3 in mouse heart tissue lysate with SIRT3 antibody at (A) 1 and (B) 2 ug/mL. Chicken Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SIRT3 antibody

  Anti-SIRT3 Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm. Positive control: Human brain and liver cancer tissue . Recommended dilution: 1/15-50  The image on the left is immunohistochemistry of paraffin-embedded human brain tissue using SIRT3 antibody at dilution 1/15, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

SIRT3 antibody

  Anti-SIRT3 Rabbit Polyclonal Antibody  
Predicted band size: 44 kDa . Positive control: Mouse brain and human liver cancer tissue lysate. Recommended dilution: 1/500-2000. (Gel: 12%SDS-PAGE Lane1: Mouse brain tissue lysate Lane2: Human liver cancer tissue lysate Lysates: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 90 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SIRT3 antibody

  Rabbit polyclonal SIRT3 antibody  
Western blot analysis of extracts from COLO205 cells, using SIRT3 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SIRT3 antibody

  Rabbit Polyclonal Anti-SIRT3 Antibody  
WB Suggested Anti-SIRT3 Antibody Titration: 2.5ug/ml; ELISA Titer: 1:312500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SIRT3 antibody

  Rabbit Polyclonal Anti-SIRT3 Antibody  
WB Suggested Anti-SIRT3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Human Thymus Rabbit IgG Purified Hu P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

SIRT3 antibody

  Rabbit Polyclonal Anti-SIRT3 Antibody  
WB Suggested Anti-SIRT3 Antibody; Titration: 5 ug/ml; Positive Control: Fetal thymus Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SIRT3 antibody

  Rabbit Polyclonal Anti-SIRT3 antibody  
Western blot analysis of SIRT3 expression in Mouse Heart lysate Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SIRT3 (C-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

SIRT3 - human gene knockout kit via CRISPR

SIRT3 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

SIRT3 - human gene knockout kit via CRISPR

SIRT3 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

SIRT3 - human gene knockout kit via CRISPR

SIRT3 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

SIRT3 - human gene knockout kit via CRISPR

SIRT3 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Sirt3 - mouse gene knockout kit via CRISPR

Sirt3 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Sirt3 - mouse gene knockout kit via CRISPR

Sirt3 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Sirt3 - mouse gene knockout kit via CRISPR

Sirt3 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Sirt3 - mouse gene knockout kit via CRISPR

Sirt3 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

SIRT3 (full length, N-term HIS tag, transcript variant 2)

  Purified recombinant protein of Human sirtuin 3 (SIRT3), nuclear gene encoding mitochondrial protein, transcript variant 2, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug  
SIRT3 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

SIRT3 (118-399, His-tag)

SIRT3 Human Purified > 95 % by SDS - PAGE E. coli
0.25 mg / €750.00
  Acris Antibodies GmbH

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