SIRT5 antibody

Principal name

SIRT5 antibody

Alternative names for SIRT5 antibody

SIRT-5, SIR2-like protein 5, SIR2L5, sirtuin type 5, sirtuin 5, sirtuin-5, sirtuin (silent mating type information regulation 2 homolog) 5 (S. cerevisiae), NAD-dependent deacetylase sirtuin-5

SwissProt ID

Q3ZBQ0 (Bovin), Q68FX9 (Rat), Q8K2C6 (Mouse), Q9NXA8 (Human)

Gene ID

23408 (SIRT5), 68346 (Sirt5)

Available reactivities

Bov (Bovine), Can (Canine), Chk (Chicken), Hu (Human), Ms (Mouse), Por (Porcine), Rt (Rat), Ze (Zebrafish), African clawed frog

Available hosts

Rabbit, Mouse, Chicken

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of SIRT5 antibody

SIRT5 is included in class III of the sirtuin family which ischaracterized by a sirtuin core domain. Human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class III of the sirtuin family. Alternative splicing of this gene results in two transcript variants.

General readings

Zhou,Y., et al. (2012) J. Biol. Chem. 287 (34), 28307-28314
Fischer,F., et al. (2012) PLoS ONE 7 (9), E45098

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SIRT5 antibody

  SIRT5 mouse monoclonal antibody, clone OTI2F1 (formerly 2F1)  
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-SIRT5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503232) Mouse IgG1 OTI2F1 Purified Hu, Rt F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SIRT5 antibody

  SIRT5 mouse monoclonal antibody, clone OTI 1G9 (formerly 1G9)  
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SIRT5 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human). Mouse IgG2b OTI 1G9 Purified Hu F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SIRT5 antibody

  SIRT5 mouse monoclonal antibody, clone OTI1A6 (formerly 1A6)  
Immunofluorescent staining of HeLa cells using anti-SIRT5 mouse monoclonal antibody (TA503281). Mouse IgG1 OTI1A6 Purified Hu F, ICC/IF, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

SIRT5 antibody

  SIRT5 mouse monoclonal antibody, clone OTI2A11 (formerly 2A11)  
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-SIRT5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503282) Mouse IgG1 OTI2A11 Purified Hu F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SIRT5 (C-term) antibody

  Chicken Polyclonal SIRT5 Antibody  
Western blot analysis of SIRT5 in human liver tissue lysate with SIRT5 antibody at 1 ug/mL. Chicken Aff - Purified Hu, Ms E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SIRT5 antibody

  Anti-SIRT5 Rabbit Polyclonal Antibody  
Predicted band size: 22 kDa . Positive control: Mouse heart tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 seconds) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SIRT5 antibody

  Anti-SIRT5 Rabbit Polyclonal Antibody  
Predicted band size: 22 kDa . Positive control: Mouse heart tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SIRT5 antibody

  Rabbit Polyclonal Sirtuin 5/SIRT5 Antibody  
Western Blot: SIRT5 Antibody [TA336344] - Analysis of SIRT5 in A) human, B) mouse and C) rat intestine cell lysate using this antibody at 2 ug/ml. Rabbit Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SIRT5 antibody

  SIRT5 mouse monoclonal antibody, clone OTI2C4 (formerly 2C4)  
Anti-SIRT5 mouse monoclonal antibody (TA503233) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SIRT5(RC200189). Mouse IgG1 OTI2C4 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SIRT5 antibody

  SIRT5 mouse monoclonal antibody, clone OTI 1G5 (formerly 1G5)  
Anti-SIRT5 mouse monoclonal antibody (TA503280) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SIRT5(RC200189). Mouse IgG1 OTI 1G5 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SIRT5 antibody

  SIRT5 mouse monoclonal antibody, clone OTI2E10 (formerly 2E10)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIRT5 (RC200189, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIRT5. Mouse IgG2a OTI2E10 Purified Hu F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SIRT5 antibody

  SIRT5 mouse monoclonal antibody, clone OTI 2E12 (formerly 2E12)  
Anti-SIRT5 mouse monoclonal antibody (TA503292) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SIRT5(RC200189). Mouse IgG1 OTI 2E12 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SIRT5 antibody

  SIRT5 mouse monoclonal antibody, clone OTI1B6 (formerly 1B6)  
Anti-SIRT5 mouse monoclonal antibody (TA503315) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SIRT5(RC200189). Mouse IgG1 OTI1B6 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SIRT5 (30-46) antibody

Kidney: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
50 µg / €470.00
  Acris Antibodies GmbH

SIRT5 (C-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

SIRT5 - human gene knockout kit via CRISPR

SIRT5 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

SIRT5 - human gene knockout kit via CRISPR

SIRT5 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

SIRT5 - human gene knockout kit via CRISPR

SIRT5 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

SIRT5 - human gene knockout kit via CRISPR

SIRT5 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Sirt5 - mouse gene knockout kit via CRISPR

Sirt5 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

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