SIRT5 antibody

Principal name

SIRT5 antibody

Alternative names for SIRT5 antibody

SIRT-5, SIR2-like protein 5, SIR2L5, sirtuin type 5, sirtuin 5, sirtuin-5, sirtuin (silent mating type information regulation 2 homolog) 5 (S. cerevisiae), NAD-dependent deacetylase sirtuin-5

SwissProt ID

Q3ZBQ0 (Bovin), Q68FX9 (Rat), Q8K2C6 (Mouse), Q9NXA8 (Human)

Gene ID

23408 (SIRT5), 68346 (Sirt5)

Available reactivities

Bov (Bovine), Can (Canine), Chk (Chicken), Hu (Human), Ms (Mouse), Por (Porcine), Rt (Rat), Ze (Zebrafish), African clawed frog

Available hosts

Rabbit, Mouse, Chicken

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Frozen Sections (C), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of SIRT5 antibody

SIRT5 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class III of the sirtuin family. Alternative splicing of this gene results in two transcript variants. Transcript variant (2) contains a distinct 5' UTR, C-terminal coding region, and 3' UTR as compared to transcript variant 1. This variant encodes isoform 2 which has a distinct and shorter C-terminus than isoform 1.

General readings

Zhou,Y., et al. (2012) J. Biol. Chem. 287 (34), 28307-28314
Fischer,F., et al. (2012) PLoS ONE 7 (9), E45098

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SIRT5 antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-SIRT5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503232) Mouse IgG1 OTI2F1 Purified Hu, Rt F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SIRT5 antibody

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SIRT5 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human). Mouse IgG2b OTI 1G9 Purified Hu F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SIRT5 antibody

Immunofluorescent staining of HeLa cells using anti-SIRT5 mouse monoclonal antibody (TA503281). Mouse IgG1 OTI1A6 Purified Hu F, ICC/IF, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

SIRT5 antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-SIRT5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503282) Mouse IgG1 OTI2A11 Purified Hu F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SIRT5 (C-term) antibody

Western blot analysis of SIRT5 in human liver tissue lysate with SIRT5 antibody at 1 ug/mL. Chicken Aff - Purified Hu, Ms E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SIRT5 antibody

Predicted band size: 22 kDa . Positive control: Mouse heart tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 seconds) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SIRT5 antibody

Predicted band size: 22 kDa . Positive control: Mouse heart tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SIRT5 antibody

Western Blot: SIRT5 Antibody [TA336344] - Analysis of SIRT5 in A) human, B) mouse and C) rat intestine cell lysate using this antibody at 2 ug/ml. Rabbit Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SIRT5 antibody

Anti-SIRT5 mouse monoclonal antibody (TA503233) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SIRT5(RC200189). Mouse IgG1 OTI2C4 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SIRT5 antibody

Anti-SIRT5 mouse monoclonal antibody (TA503280) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SIRT5(RC200189). Mouse IgG1 OTI 1G5 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SIRT5 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIRT5 (RC200189, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIRT5. Mouse IgG2a OTI2E10 Purified Hu F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SIRT5 antibody

Anti-SIRT5 mouse monoclonal antibody (TA503292) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SIRT5(RC200189). Mouse IgG1 OTI 2E12 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SIRT5 antibody

Anti-SIRT5 mouse monoclonal antibody (TA503315) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SIRT5(RC200189). Mouse IgG1 OTI1B6 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SIRT5 (30-46) antibody

Kidney: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
50 µg / €470.00
  Acris Antibodies GmbH

SIRT5 (C-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P, WB

Regular Price: 0.4 ml / €370.00

Special Price: 0.4 ml / €231.00

  Acris Antibodies GmbH

SIRT5 control peptide

SIRT5
50 µg / €160.00
  Acris Antibodies GmbH

SIRT5 antibody

Western Blot analysis of SIRT5 expression in transfected 293T cell line (H00023408-T01) by SIRT5 MaxPab polyclonal antibody.

Lane 1: SIRT5 transfected lysate(34.1 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

SIRT5 antibody

SIRT5 Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

SIRT5 antibody

Western Blot analysis of SIRT5 expression in transfected 293T cell line (H00023408-T01) by SIRT5 MaxPab polyclonal antibody.

Lane 1: SIRT5 transfected lysate(33.90 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

SIRT5 antibody

Western blot analysis of SIRT5 in A ) human, B ) mouse and C ) rat intestine cell lysate. Using SIRT5 polyclonal antibody ( Cat # PAB0206 ) at 2 µg/ml. Rabbit Hu, Ms, Rt WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

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