SIT1 / SIT antibody

Principal name

SIT1 / SIT antibody

Alternative names for SIT1 / SIT antibody

Signaling threshold-regulating transmembrane adapter 1, SHP2-interacting transmembrane adapter protein, Suppression-inducing transmembrane adapter 1

SwissProt ID

P39980 (Yeast), Q5M869 (Rat), Q8C503 (Mouse), Q9Y3P8 (Human)

Gene ID

27240 (SIT1), 54390 (Sit1), 500449

Available reactivities

Hu (Human), Ms (Mouse)

Available hosts

Mouse, Rabbit

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Flow Cytometry (F)

Background of SIT1 / SIT antibody

Kit Component:
- KN315809G1, Sit1 gRNA vector 1 in pCas-Guide vector
- KN315809G2, Sit1 gRNA vector 2 in pCas-Guide vector
- KN315809D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

Pfrepper K., et al. (2001) Eur. J. Immunol. 31:1825-1836.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SIT1 / SIT (C-term) antibody

Western blot analysis of SIT1 / SIT Antibody (C-term) Cat.-No AP53920PU-N in MDA-MB453 cell line lysates (35ug/lane). This demonstrates the SIT1 antibody detected the SIT1 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

SIT1 / SIT antibody

Western blot of human Jurkat T cell line lysate (1% laurylmaltoside); non-reduced sample, immunostained by mAb SIT-01 and goat anti-mouse IgG (H+L)-HRP conjugate. Mouse IgG1 SIT-01 Purified Hu, Ms F, IP, WB
0.1 mg / €240.00
  Acris Antibodies GmbH

SIT1 / SIT antibody

Western blot of human Jurkat T cell line lysate (1% laurylmaltoside); non-reduced sample, immunostained by mAb SIT-01 and goat anti-mouse IgG (H+L)-HRP conjugate. Mouse IgG1 SIT-01 PE Hu, Ms F
0.1 mg / €370.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

SIT1 - human gene knockout kit via CRISPR

SIT1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

SIT1 - human gene knockout kit via CRISPR

SIT1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

SIT1 - human gene knockout kit via CRISPR

SIT1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

SIT1 - human gene knockout kit via CRISPR

SIT1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Sit1 - mouse gene knockout kit via CRISPR

Sit1 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Sit1 - mouse gene knockout kit via CRISPR

Sit1 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Sit1 - mouse gene knockout kit via CRISPR

Sit1 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Sit1 - mouse gene knockout kit via CRISPR

Sit1 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

SIT1 / SIT

  Recombinant protein of human signaling threshold regulating transmembrane adaptor 1 (SIT1)  
SIT1 / SIT Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

SIT1 / SIT

  Recombinant protein of human signaling threshold regulating transmembrane adaptor 1 (SIT1)  
SIT1 / SIT Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
10 µg / €399.00
  OriGene Technologies, Inc.

SIT1 / SIT (62-196, HIS-Tag)

Recombinant human SIT1, 62-196aa, His-tagged Human Purified > 90 % by SDS-PAGE E. coli
50 µg / €750.00
  Acris Antibodies GmbH

SIT1 / SIT (62-196, HIS-Tag)

Recombinant human SIT1, 62-196aa, His-tagged Human Purified > 90 % by SDS-PAGE E. coli
10 µg / €300.00
  Acris Antibodies GmbH

Lysates

Catalog No.    

SIT1 overexpression lysate

SIT1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, SIT1 (mGFP-tagged) - Human signaling threshold regulating transmembrane adaptor 1 (SIT1), 200 uL, >10^7 TU/mL

Lenti ORF particles, SIT1 (mGFP-tagged) - Human signaling threshold regulating transmembrane adaptor 1 (SIT1), 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, SIT1 (Myc-DDK tagged) - Human signaling threshold regulating transmembrane adaptor 1 (SIT1) , 200 uL, >10^7 TU/mL

Lenti ORF particles, SIT1 (Myc-DDK tagged) - Human signaling threshold regulating transmembrane adaptor 1 (SIT1) , 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Sit1 (GFP-tagged) - Mouse suppression inducing transmembrane adaptor 1 (Sit1), 200 uL, >10^7 TU/mL

Lenti ORF particles, Sit1 (GFP-tagged) - Mouse suppression inducing transmembrane adaptor 1 (Sit1), 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, Sit1 (Myc-DDK-tagged) - Mouse suppression inducing transmembrane adaptor 1 (Sit1), 200 uL, >10^7 TU/mL

Lenti ORF particles, Sit1 (Myc-DDK-tagged) - Mouse suppression inducing transmembrane adaptor 1 (Sit1), 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

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