SIT1 / SIT antibody

Principal name

SIT1 / SIT antibody

Alternative names for SIT1 / SIT antibody

Signaling threshold-regulating transmembrane adapter 1, SHP2-interacting transmembrane adapter protein, Suppression-inducing transmembrane adapter 1

SwissProt ID

P39980 (Yeast), Q5M869 (Rat), Q8C503 (Mouse), Q9Y3P8 (Human)

Gene ID

27240 (SIT1), 54390 (Sit1)

Available reactivities

Hu (Human)

Available hosts

Mouse, Rabbit, Goat

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Flow Cytometry (F), Enzyme Immunoassay (E)

Background of SIT1 / SIT antibody

SIT is a nbsp40 kD disulfide-linked protein also known as SHP2-interacting transmembrane adaptor protein. SIT is a membrane protein containing a very short extracellular domain (18 amino acids). SIT is expressed in T cells, but is not expressed in monocytes. SIT is a signaling adaptor molecule that negatively regulates T cell activation through SHP-2 interactions. The SIT protein is phosphorylated by Src kinases and has been shown to associate with SHP2, Csk, Zap70, Syk, Fyn, Lck, and Grb2. SIT is also heavily glycosylated and treatment with endoglycosidase has been shown to reduce the apprarent molecular weight to approximately 20 kD. The SIT-40 antibody recognizes the intracellular region of human SIT and has been shown to be useful for Western blotting.

General readings

Simeoni L, Posevitz V, Kolsch U, Meinert I, Bruyns E, Pfeffer K, Reinhold D, Schraven B: The transmembrane adapter protein SIT regulates thymic development and peripheral T-cell functions. Mol Cell Biol. 2005 Sep;25(17):7557-68.
Hubener C, Mincheva A, Lichter P, Schraven B, Bruyns E: Complete sequence, genomic organization, and chromosomal localization of the human gene encoding the SHP2-interacting transmembrane adaptor protein (SIT). Immunogenetics. 2001 May-Jun;53(4):337-41.
Marie-Cardine A, Kirchgessner H, Bruyns E, Shevchenko A, Mann M, Autschbach F, Ratnofsky S, Meuer S, Schraven B: SHP2-interacting transmembrane adaptor protein (SIT), a novel disulfide-linked dimer regulating human T cell activation. J Exp Med. 1999 Apr 19;189(8):1181-94.
Horejsi V, Zhang W, Schraven B.: Transmembrane adaptor proteins: organizers of immunoreceptor signalling. Nat Rev Immunol. 2004 Aug;4(8):603-16.
Tedoldi S, Paterson JC, Hansmann ML, Natkunam Y, Rüdiger T, Angelisova P, Du MQ, Roberton H, Roncador G, Sanchez L, Pozzobon M, Masir N, Barry R, Pileri S, Mason DY, Marafioti T, Horejsí V.: Transmembrane adaptor molecules: a new category of lymphoid-cell markers. Blood. 2006 Jan 1;107(1):213-21.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SIT1 / SIT (C-term) antibody

Western blot analysis of SIT1 / SIT Antibody (C-term) Cat.-No AP53920PU-N in MDA-MB453 cell line lysates (35ug/lane). This demonstrates the SIT1 antibody detected the SIT1 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

SIT1 / SIT antibody

Western blot of human Jurkat T cell line lysate (1% laurylmaltoside); non-reduced sample, immunostained by mAb SIT-01 and goat anti-mouse IgG (H+L)-HRP conjugate. Mouse IgG1 SIT-01 Purified Hu, Ms F, IP, WB
0.1 mg / €240.00
  Acris Antibodies GmbH

SIT1 / SIT antibody

Western blot of human Jurkat T cell line lysate (1% laurylmaltoside); non-reduced sample, immunostained by mAb SIT-01 and goat anti-mouse IgG (H+L)-HRP conjugate. Mouse IgG1 SIT-01 PE Hu, Ms F
0.1 mg / €370.00
  Acris Antibodies GmbH

SIT1 / SIT antibody

SIT1 / SIT Mouse IgG1 SIT-01 Hu F, IP, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

SIT1 / SIT antibody

SIT1 / SIT Mouse IgG1 SIT-01 Hu F, IP, WB
0.1 mg / €470.00
  Abnova Taiwan Corp.

SIT1 / SIT antibody

Extracts from peripheral blood mononuclear cells were resolved by electrophoresis, transferred to nitrocellulose, and probed with mouse monoclonal antibody against SIT. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system. Mouse IgG1 SIT-40 Purified Hu WB
0.1 mg / €440.00
  Novus Biologicals Inc.

SIT1 / SIT antibody

SIT1 / SIT Mouse IgG1 SIT-01 Aff - Purified Hu F, IP, WB
0.1 mg / €340.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

SIT1 / SIT

SIT1 / SIT Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

SIT1 / SIT

SIT1 / SIT Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
10 µg / €399.00
  OriGene Technologies, Inc.

SIT1 / SIT (62-196, HIS-Tag)

Recombinant human SIT1, 62-196aa, His-tagged Human Purified > 90 % by SDS-PAGE E. coli
50 µg / €750.00
  Acris Antibodies GmbH

SIT1 / SIT (62-196, HIS-Tag)

Recombinant human SIT1, 62-196aa, His-tagged Human Purified > 90 % by SDS-PAGE E. coli
10 µg / €300.00
  Acris Antibodies GmbH

SIT1 / SIT

SIT1 / SIT Human
10 µg / €380.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

SIT1 overexpression lysate

SIT1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

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