SIV Protein Nef antibody

Principal name

SIV Protein Nef antibody

Alternative names for SIV Protein Nef antibody

Simian immunodeficiency virus Negative factor, F-protein, 3'ORF

SwissProt ID

P12482 (Sivs4)

Ncbi ID

11737

Available reactivities

Mky (Monkey)

Available hosts

Sheep, Mouse

Available applications

Western blot / Immunoblot (WB), Frozen Sections (C), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)

Background of SIV Protein Nef antibody

The Simian Immunodeficiency Virus (SIV) nef protein (SIV nef) is expressed at high levels early and throughout infection. This protein is a determinant of pathogenesis and disease progression in adult macaques infected with SIV.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SIV Protein Nef Nef (71-80) antibody

SIV Protein Nef Mouse IgG1 17 (04-001) Aff - Purified Mky C, E, ICC/IF, IP, WB
0.1 mg / €400.00
  Acris Antibodies GmbH

SIV Protein Nef antibody

THP-I cells (Lane 1) and THP-I cells transduced to express SIVmm239Nef (Lane 2) were lysed in 50 mM Tris, pH: 8.0, containing 5% glycerol, 133 mM NaCI, 2 mM EDTA, 2 µg/ml each of leupeptin and aprotinin, 2 mM Na3VO4, 1 mM PMSF, and 0.5% NP-40. After centrifugation at 15,000xg for 20 minutes the supernatant fractions (200 µg protein) were analyzed by SDS PAGE (11% acrylamide), blotted to nitrocellulose, and detected by ECL. Primary antibody was sheep anti-SIVmm239Nef (1 µg/ml) and secondary antibody was rabbit anti-sheep conjugated to HRP (1:10,000) from Chemicon International, Inc. Kodak XAR film was exposed to the blot for 15 seconds. Sheep IgG Purified WB
0.25 mg / €360.00
  Acris Antibodies GmbH

2 Item(s)

per page
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