Skeletal muscle Troponin I antibody

Principal name

Skeletal muscle Troponin I antibody

Alternative names for Skeletal muscle Troponin I antibody

TNNI1

SwissProt ID

P02645 (Rabit), P13413 (Rat), P19237 (Human), Q9WUZ5 (Mouse)

Gene ID

7135 (TNNI1), 21952 (Tnni1), 29388

Available reactivities

Hu (Human), Chk (Chicken), Ms (Mouse), Por (Porcine), Rb (Rabbit), Rt (Rat), Ze (Zebrafish), African clawed frog, Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Gt (Goat)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), ELISA (capture) (E(capture)), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P)

Background of Skeletal muscle Troponin I antibody

Troponin proteins associate with tropomyosin and regulate the calcium sensitivity of the myofibril contractile apparatus of striated muscles. Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. The TnI-fast and TnI-slow genes are expressed in fast-twitch and slow-twitch skeletal muscle fibers, respectively, while the TnI-cardiac gene is expressed exclusively in cardiac muscle tissue. This gene encodes the Troponin-I-skeletal-slow-twitch protein. This gene is expressed in cardiac and skeletal muscle during early development but is restricted to slow-twitch skeletal muscle fibers in adults. The encoded protein prevents muscle contraction by inhibiting calcium-mediated conformatiol changes in actin-myosin complexes. [provided by RefSeq, Jul 2008].

General readings

Corin S.J., et al. (1994) J. Biol. Chem. 269:10651-10659Wade R., et al. (1990) Genomics. 7:346-357

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Skeletal muscle Troponin I (N-term) antibody

AP32707PU-N TNNI1 Antibody (0.03 μg/ml) staining of Human Skeletal Muscle lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Hu, Por E, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Skeletal muscle Troponin I antibody

  Rabbit Polyclonal Anti-TNNI1 Antibody  
WB Suggested Anti-TNNI1 Antibody Titration: 0.2-1 ug/ml; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Gt, Hu, Ms, Por, Rb, Rt P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Skeletal muscle Troponin I antibody

  Rabbit Polyclonal Anti-TNNI1 Antibody  
WB Suggested Anti-TNNI1 Antibody Titration: 2.5 ug/ml; Positive Control: Jurkat cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Gt, Hu, Ms, Por, Rb, Rt, Ze WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Skeletal muscle Troponin I antibody

  TNNI1 mouse monoclonal antibody, clone OTI8H8 (formerly 8H8)  
Immunohistochemical staining of paraffin-embedded Human muscle tissue using anti-TNNI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807521) Mouse IgG1 OTI8H8 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Skeletal muscle Troponin I antibody

Skeletal muscle, Human: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2b 12F10 Purified Hu P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Skeletal muscle Troponin I antibody

The extracts of mouse muscle (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TNNI1 antibody (1:500 ~ 1:5000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG2b AT36E7 Purified Hu E, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

Skeletal muscle Troponin I antibody

The extracts of mouse muscle (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TNNI1 antibody (1:500 ~ 1:5000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG2b AT36E7 Purified Hu E, WB
50 µl / €220.00
  Acris Antibodies GmbH

Skeletal muscle Troponin I antibody

Skeletal muscle Troponin I Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

Skeletal muscle Troponin I antibody

Detection limit for recombinant GST tagged TNNI1 is approximately 1ng/ml as a capture antibody. Mouse IgG2b 4G6 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

Skeletal muscle Troponin I antibody

Human Skeletal Muscle; Immunohistochemistry with Human Skeletal Muscle lysate tissue at an antibody concentration of 5.0ug/ml using anti-TNNI1 antibody (ARP42117_P050); TNNI1 antibody - N-terminal region (ARP42117_P050) in Human Skeletal Muscle cells using Immunohistochemistry Rabbit Aff - Purified Hu, Ms, Por, Rb, Rt P, WB
50 µg / €350.00
  AVIVA Systems Biology

Skeletal muscle Troponin I antibody

Human Jurkat; WB Suggested Anti-TNNI1 Antibody Titration: 2.5ug/ml. Positive Control: Jurkat cell lysate; TNNI1 antibody - middle region (ARP42118_T100) in Human Jurkat cells using Western Blot Rabbit Aff - Purified African clawed frog, Chk, Hu, Ms, Por, Rb, Rt, Ze WB
0.1 mg / €310.00
  AVIVA Systems Biology

Skeletal muscle Troponin I antibody

Skeletal muscle Troponin I Mouse IgG2b 7G2 Purified Hu E, E(detection), WB
1 mg / €410.00
  HyTest Ltd.

Skeletal muscle Troponin I antibody

Skeletal muscle Troponin I Mouse IgG2b 12F10 Purified Hu E, E(capture), WB
1 mg / €410.00
  HyTest Ltd.

CRISPR

Catalog No. Species Donor Vector  

TNNI1 - human gene knockout kit via CRISPR

TNNI1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

TNNI1 - human gene knockout kit via CRISPR

TNNI1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

TNNI1 - human gene knockout kit via CRISPR

TNNI1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

TNNI1 - human gene knockout kit via CRISPR

TNNI1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Skeletal muscle Troponin I

  Purified recombinant protein of Homo sapiens troponin I type 1 (skeletal, slow) (TNNI1)  
Skeletal muscle Troponin I Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Skeletal muscle Troponin I (full length, C-term DDK tag)

  Purified recombinant protein of Human troponin I type 1 (skeletal, slow) (TNNI1), full length, with C-terminal DDK tag, expressed in sf9, 20ug  
Skeletal muscle Troponin I Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
SF9 cells
20 µg / €399.00
  OriGene Technologies, Inc.

Skeletal muscle Troponin I (1-187, His-tag)

Skeletal muscle Troponin I Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

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