SKP1 / OCP2 antibody

Principal name

SKP1 / OCP2 antibody

Alternative names for SKP1 / OCP2 antibody

SKP1A, p19skp1, p19A, OCP-2, OCP-II, EMC19, TCEB1L, S-phase kinase-associated protein 1, Cyclin A/CDK2-associated protein p19, RNA polymerase II elongation factor-like protein, Organ of Corti protein 2, Transcription elongation factor B

SwissProt ID

P63208 (Human), P63209 (Cavpo), Q3ZCF3 (Bovin), Q5ZKF5 (Chick), Q6PEC4 (Rat), Q71U00 (Xenla), Q9WTX5 (Mouse)

Gene ID

6500 (SKP1), 21402 (Skp1a), 287280 (Skp1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), ELISA (detection) (E(detection)), Flow Cytometry (F)

Background of SKP1 / OCP2 antibody

The critical role that the family of regulatory proteins known as cyclins, plays in eukaryotic cell cycle regulation is well established. The best characterized cyclin complex is the mitotic cyclin B/Cdc2 p34 kinase, the active component of MPF (maturation promoting factor). Cyclin A accumulates prior to cyclin B in the cell cycle, appears to be involved in control of S phase and has been shown to associate with cyclin-dependent kinase-2 (Cdk2). In addition, cyclin A has been implicated in cell transformation and is found in complexes with E1A, transcription factors DP-1 and E2F and retinoblastoma protein p110. Two cyclin A-Cdk2 complex binding proteins, Skp1 p19 and Skp2 p45, have been described. Although the Skps (S phase kinase-associated proteins) associate with the active cyclin A-Cdk2 complex, they do not exhibit any regulatory effects on the complex. Abolition of Skp2 p45 function by either microinjection of anti-p45 antibodies or addition of antisense oligonucleotides prevents entry into S phase of both normal and transformed cells.

General readings

Specific Reference(s):
1. Michel,J.J. and Xiong,Y. (1998) Human CUL-1, but not other cullin family members, selectively interacts with SKP1 to form a complex with SKP2 and cyclin A. Cell Growth Differ. 9 (6), 435-449.
2. Ohta,T., Michel,J.J., Schottelius,A.J. and Xiong,Y. (1999) ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity. Mol. Cell 3 (4), 535-541.
3. Zhang,H., Kobayashi,R., Galaktionov,K. and Beach,D. (1995) p19Skp1 and p45Skp2 are essential elements of the cyclin A-CDK2 S phase kinase. Cell 82 (6), 915-925.
4. Bai C, Sen P, Hofmann K, Ma L, Goebl M, Harper JW, Elledge SJ. (1996) SKP1 connects cell cycle regulators to the ubiquitin proteolysis machinery through a novel motif, the F-box. Cell. 26;86(2):263-74.
5. Connelly C, Hieter P. (1996) Budding yeast SKP1 encodes an evolutionarily conserved kinetochore protein required for cell cycle progression. Cell. 86(2):275-85.
6. Feldman RM, Correll CC, Kaplan KB, Deshaies RJ. (1997) A complex of Cdc4p, Skp1p, and Cdc53p/cullin catalyzes ubiquitination of the phosphorylated CDK inhibitor Sic1p. Cell. 91(2):221-30.
7. Liu J, Furukawa M, Matsumoto T, Xiong Y. (2002) NEDD8 modification of CUL1 dissociates p120(CAND1), an inhibitor of CUL1-SKP1 binding and SCF ligases. Mol Cell. (6):1511-8.
General Reference(s):
1. Jentsch S, Pyrowolakis G. (2000) Ubiquitin and its kin: how close are the family ties? Trends Cell Biol. 10(8):335-42.
2. Sowden,J., Morrison,K., Schofield,J., Putt,W. and Edwards,Y. (1995) A novel cDNA with homology to an RNA polymerase II elongation factor maps to human chromosome 5q31 (TCEB1L) and to mouse chromosome 11 (Tceb1l). Genomics 29 (1), 145-151.
3. Liang,Y., Chen,H., Asher,J.H. Jr., Chang,C.C. and Friedman,T.B. (1997) Human inner ear OCP2 cDNA maps to 5q22-5q35.2 with related sequences on chromosomes 4p16.2-4p14, 5p13-5q22, 7pter-q22, 10 and 12p13-12qte. Gene 184 (2), 163-167.
4. Dias,D.C., Dolios,G., Wang,R. and Pan,Z.Q. (2002) CUL7: A DOC domain-containing cullin selectively binds Skp1.Fbx29 to form an SCF-like complex. Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16601-16606.
5. Matsuzawa,S.I. and Reed,J.C. (2001) Siah-1, SIP, and Ebi collaborate in a novel pathway for beta-catenin degradation linked to p53 responses. Mol. Cell 7 (5), 915-926.
6. Schulman,B.A., Carrano,A.C., Jeffrey,P.D., Bowen,Z., Kinnucan,E.R., Finnin,M.S., Elledge,S.J., Harper,J.W., Pagano,M. and Pavletich,N.P. (2000) Insights into SCF ubiquitin ligases from the structure of the Skp1-Skp2 complex. Nature 408 (6810), 381-386.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SKP1 / OCP2 antibody

Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using SKP1 mouse mAb with DAB staining. Mouse IgG1 4E11 Ascites Hu E, F, ICC/IF, P, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

SKP1 / OCP2 antibody

Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues (left) and cervical cancer tissues (right) using SKP1 antibody Cat.-No AM06720SU-N with DAB staining. Mouse IgG1 1H9 Ascites Hu E, F, P, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

SKP1 / OCP2 antibody

  Anti-SKP1 rabbit polyclonal antibody  
Predicted band size: 19kDa. Positive control: Hela cell lysate. Recommended dilution: 1/500-2000. (Gel: 12%SDS-PAGE Lysate:40 ug Primary antibody: 1/400 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/8000 dilution Exposure time: 20 seconds) Rabbit IgG Purified Hu, Ms, Rt P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SKP1 / OCP2 antibody

  Anti-SKP1 rabbit polyclonal antibody  
Predicted band size: 19kDa. Positive control: Mouse brain tissue and NIH/3T3 cell lysate. Recommended dilution: 1/500-2000. (Gel: 12%SDS-PAGE Lane1: Mouse brain tissue lysate Lane2: NIH/3T3 cell lysate Lysates: 40 ug per lane Primary antibody: 1/550 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/8000 dilution Exposure time: 20 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SKP1 / OCP2 antibody

  Rabbit anti-SKP1 Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using SKP1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

SKP1 / OCP2 antibody

  Rabbit Polyclonal anti-SKP1 p19 Antibody  
Western Blot analysis of NIH3T3 cell lysate with SKP1 p19 antibody Rabbit Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SKP1 / OCP2 antibody

Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Brain, Cerebellum. Rabbit Hu E, IP, P, WB
50 µl / €470.00
  Acris Antibodies GmbH

SKP1 / OCP2 (Center) antibody

Western blot analysis of anti-SKP1A (AP11747PU-N) in CEM cell line lysate (35 µg/lane). SKP1A (arrow) was detected using the purified Pab (1:60 dilution). Rabbit Ig Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

SKP1 / OCP2 antibody

Immunohistochemistry analyzes of Skp1 p19 antibody (AP20271PU-N) in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

SKP1 / OCP2 (C-term) antibody

Figure 1. Conjugation pathways for ubiquitin and ubiquitin-like modifiers (UBLs). Most modifiers mature by proteolytic processing from inactive precursors (a; amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins by RUB involves SCF(SKP1/cullin-1/F-box protein) /CBC(cullin-2/elongin B/elonginC) -like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin. Data contributed by S.Jentsch, see references above. Rabbit Serum Hu E, IP, WB
0.1 ml / €450.00
  Acris Antibodies GmbH

SKP1 / OCP2 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using SKP1A/p19 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €230.00
  Assay Biotech

SKP1 / OCP2 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using SKP1A/p19 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €330.00
  Assay Biotech

CRISPR

Catalog No. Species Donor Vector  

Skp1a - mouse gene knockout kit via CRISPR

Skp1a - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Skp1a - mouse gene knockout kit via CRISPR

Skp1a - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Skp1a - mouse gene knockout kit via CRISPR

Skp1a - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Skp1a - mouse gene knockout kit via CRISPR

Skp1a - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

SKP1 / OCP2 (transcript variant 2)

  Recombinant protein of human S-phase kinase-associated protein 1 (SKP1), transcript variant 2  
SKP1 / OCP2 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

SKP1 / OCP2 (full length, N-term HIS tag, transcript variant 1)

  Purified recombinant protein of Human S-phase kinase-associated protein 1 (SKP1), transcript variant 1, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug  
SKP1 / OCP2 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

SKP1 / OCP2 (1-160)

SKP1 / OCP2 Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €670.00
  Acris Antibodies GmbH

SKP1 / OCP2 (1-160)

SKP1 / OCP2 Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €250.00
  Acris Antibodies GmbH

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