SLC12A1 antibody

Principal name

SLC12A1 antibody

Alternative names for SLC12A1 antibody

NKCC2, Solute carrier family 12 member 1, Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2, Kidney-specific Na-K-Cl symporter

SwissProt ID

P55014 (Mouse), P55015 (Rabit), P55016 (Rat), Q13621 (Human)

Gene ID

6557 (SLC12A1), 25065 (Slc12a1)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Rb (Rabbit), Rt (Rat), Mky (Monkey), Bt (Bat), Eq (Equine), Hst (Hamster), Por (Porcine), Prim (Primate), Bear, GP (Guinea Pig)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Dot blot (Dot)

Background of SLC12A1 antibody

Electrically silent Na(+)-(K+)-Cl- transporter systems are present in a wide variety of cells and serve diverse physiological functions. In chloride secretory and absorbing epithelia, these cotransporters provide the chloride entry mechanism crucial for trans-cellular chloride transport. Renal handling of NaCl is a major determinant for homeostasis of salt and extracellular fluid volumes and consequently blood pressure. The kidney specific Na-K-co-transporter (NKCC2) facilitates and mediates the transport of Na and Cl across the luminal membrane of the thick ascending limb of the loop of Henle and the macula densa. NKCC2 mutations and lack of function caused Bartters syndrome and led to salt-wasting in neonates (1). Diuretics like bumetimide, furosemide act on NKCC2. A second physiological function of KCC2 is its involvement in tubuloglomerular feedback to control the renal blood flow. The renal-specific NKCC2 (Na+–K+–2Cl− co-transporter 2) is regulated by changes in phosphorylation state by metabolic sensing kinase (AMPK) which phosphorylates the serine126 (1). The chloride-sensitive activation of NKCC2 requires the interaction of two serine-threonine kinases, WNK3 and SPAK. WNK3 is positioned upstream of SPAK and appears to be the chloride-sensitive kinase. Elimination of WNK3’s unique SPAK-binding motif prevents its activation of NKCC2, as does the mutation of thr 96, 101, and 111 (2). The expression of NKCC2 in the thick ascending loop of Henle is decreased by cGMP mediated by PDE2A (3).
The two major electroneutral sodium-chloride transporters present in the mammalian kidney, the bumetanide-sensitive Na(+)-K(+)-Cl- symporter and thiazide-sensitive Na(+)-Cl- cotransporter, and have characterized. Despite their differing sensitivities to bumetanide and thiazides and their different requirements for potassium, these approximately 115-kDa proteins share significant sequence similarity (approximately 60%) and exhibit a topology featuring 12 potential membrane-spanning helices flanked by long non-hydrophobic domains at the NH2 and COOH termini. The NKCC2 has an apparent molecular weight of around 160kDa in ascending loop of Henle (4).

General readings

1. Scott A. Fraser, et. al., Regulation of the renal-specific Na+–K+–2Cl− co-transporter NKCC2 by AMP-activated protein kinase (AMPK). Biochem J. 2007 July 1; 405 85–93.
2. Ponce-Coria J. et. al., Regulation of NKCC2 by a chloride-sensing mechanism involving the WNK3 and SPAK kinases. PNAS 2008 Jun 17;105:8458-
63. Epub 2008 Jun 11.
3. Ares GR, Caceres P, Alvarez-Leefmans FJ, Ortiz PA. cGMP decreases surface NKCC2 levels in the thick ascending limb: role of phosphodiesterase 2
(PDE2). Am J Physiol Renal Physiol. 2008 Oct;295(4):F877-87. Epub 2008 Aug 6.

11 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SLC12A1 antibody

  Anti-SLC12A1 Rabbit Polyclonal Antibody  
Predicted band size: 46 kDa . Positive control: Human fetal kidney tissue lysate. Recommended dilution: 1/200-1000. (Gel: 12%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/200 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SLC12A1 antibody

  Rabbit Polyclonal Anti-NKCC2 Antibody  
Western blot analysis of NKCC2 in rat kidney tissue using a 1:1000 dilution of the antibody Rabbit Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SLC12A1 antibody

  Rabbit Polyclonal Anti-SLC12A1 Antibody  
WB Suggested Anti-SLC12A1 Antibody Titration: 0.2-1 ug/ml; Positive Control: Jurkat cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

SLC12A1 (43-57) antibody

Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE) Goat IgG Aff - Purified Hu, Mky, Platypus P
50 µg / €470.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

SLC12A1 - human gene knockout kit via CRISPR

SLC12A1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

SLC12A1 - human gene knockout kit via CRISPR

SLC12A1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

SLC12A1 - human gene knockout kit via CRISPR

SLC12A1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

SLC12A1 - human gene knockout kit via CRISPR

SLC12A1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, SLC12A1 (mGFP-tagged) - Human solute carrier family 12 (sodium/potassium/chloride transporters), member 1 (SLC12A1), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, SLC12A1 (mGFP-tagged) - Human solute carrier family 12 (sodium/potassium/chloride transporters), member 1 (SLC12A1), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €2,200.00
  OriGene Technologies, Inc.

Lenti ORF particles, SLC12A1 (mGFP-tagged)-Human solute carrier family 12 (sodium/potassium/chloride transporters), member 1 (SLC12A1), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, SLC12A1 (mGFP-tagged)-Human solute carrier family 12 (sodium/potassium/chloride transporters), member 1 (SLC12A1), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €2,200.00
  OriGene Technologies, Inc.

Lenti ORF particles, SLC12A1 (Myc-DDK-tagged)-Human solute carrier family 12 (sodium/potassium/chloride transporters), member 1 (SLC12A1), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, SLC12A1 (Myc-DDK-tagged)-Human solute carrier family 12 (sodium/potassium/chloride transporters), member 1 (SLC12A1), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €1,980.00
  OriGene Technologies, Inc.

11 Item(s)

per page
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