SLC12A1 antibody

Principal name

SLC12A1 antibody

Alternative names for SLC12A1 antibody

NKCC2, Solute carrier family 12 member 1, Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2, Kidney-specific Na-K-Cl symporter

SwissProt ID

P55014 (Mouse), P55015 (Rabit), P55016 (Rat), Q13621 (Human)

Gene ID

6557 (SLC12A1), 25065

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Rb (Rabbit), Rt (Rat), Mky (Monkey), Eq (Equine), GP (Guinea Pig), Por (Porcine)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Dot blot (Dot), Frozen Sections (C)

Background of SLC12A1 antibody

Electrically silent Na(+)-(K+)-Cl- transporter systems are present in a wide variety of cells and serve diverse physiological functions. In chloride secretory and absorbing epithelia, these cotransporters provide the chloride entry mechanism crucial for trans-cellular chloride transport. Renal handling of NaCl is a major determinant for homeostasis of salt and extracellular fluid volumes and consequently blood pressure. The kidney specific Na-K-co-transporter (NKCC2) facilitates and mediates the transport of Na and Cl across the luminal membrane of the thick ascending limb of the loop of Henle and the macula densa. NKCC2 mutations and lack of function caused Bartters syndrome and led to salt-wasting in neonates (1). Diuretics like bumetimide, furosemide act on NKCC2. A second physiological function of KCC2 is its involvement in tubuloglomerular feedback to control the renal blood flow. The renal-specific NKCC2 (Na+–K+–2Cl− co-transporter 2) is regulated by changes in phosphorylation state by metabolic sensing kinase (AMPK) which phosphorylates the serine126 (1). The chloride-sensitive activation of NKCC2 requires the interaction of two serine-threonine kinases, WNK3 and SPAK. WNK3 is positioned upstream of SPAK and appears to be the chloride-sensitive kinase. Elimination of WNK3’s unique SPAK-binding motif prevents its activation of NKCC2, as does the mutation of thr 96, 101, and 111 (2). The expression of NKCC2 in the thick ascending loop of Henle is decreased by cGMP mediated by PDE2A (3).
The two major electroneutral sodium-chloride transporters present in the mammalian kidney, the bumetanide-sensitive Na(+)-K(+)-Cl- symporter and thiazide-sensitive Na(+)-Cl- cotransporter, and have characterized. Despite their differing sensitivities to bumetanide and thiazides and their different requirements for potassium, these approximately 115-kDa proteins share significant sequence similarity (approximately 60%) and exhibit a topology featuring 12 potential membrane-spanning helices flanked by long non-hydrophobic domains at the NH2 and COOH termini. The NKCC2 has an apparent molecular weight of around 160kDa in ascending loop of Henle (4).

General readings

1. Scott A. Fraser, et. al., Regulation of the renal-specific Na+–K+–2Cl− co-transporter NKCC2 by AMP-activated protein kinase (AMPK). Biochem J. 2007 July 1; 405 85–93.
2. Ponce-Coria J. et. al., Regulation of NKCC2 by a chloride-sensing mechanism involving the WNK3 and SPAK kinases. PNAS 2008 Jun 17;105:8458-
63. Epub 2008 Jun 11.
3. Ares GR, Caceres P, Alvarez-Leefmans FJ, Ortiz PA. cGMP decreases surface NKCC2 levels in the thick ascending limb: role of phosphodiesterase 2
(PDE2). Am J Physiol Renal Physiol. 2008 Oct;295(4):F877-87. Epub 2008 Aug 6.

16 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SLC12A1 antibody

Predicted band size: 46 kDa . Positive control: Human fetal kidney tissue lysate. Recommended dilution: 1/200-1000. (Gel: 12%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/200 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SLC12A1 antibody

Western blot analysis of NKCC2 in rat kidney tissue using a 1:1000 dilution of the antibody Rabbit Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SLC12A1 antibody

WB Suggested Anti-SLC12A1 Antibody Titration: 0.2-1 ug/ml; Positive Control: Jurkat cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

SLC12A1 control peptide

SLC12A1
0.25 mg / €250.00
  Acris Antibodies GmbH

SLC12A1 (33-55) antibody

SLC12A1 Rabbit Aff - Purified Hu, Mky, Ms, Rt Dot, E, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

SLC12A1 (43-57) antibody

Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE) Goat IgG Aff - Purified Bear, Bov, Bt, Can, Eq, Hst, Hu, Mky, Ms, Platypus, Por, Prim, Rb, Rt P
50 µg / €470.00
  Acris Antibodies GmbH

SLC12A1 antibody

Western Blot analysis of SLC12A1 expression in transfected 293T cell line by SLC12A1 monoclonal antibody (M03), clone 4H4.

Lane 1: SLC12A1 transfected lysate(46.5 KDa).
Lane 2: Non-transfected lysate. Mouse IgG2a 4H4 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SLC12A1 antibody

Human Kidney; Rabbit Anti-SLC12A1 Antibody. Catalog Number: ARP41388. Paraffin Embedded Tissue: Human Kidney. Cellular Data: Epithelial cells of renal tubule. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X; SLC12A1 antibody - N-terminal region (ARP41388_P050) in Human Kidney cells using Immunohistochemistry Rabbit Purified Bov, Can, Chk, Hu, Ms, Rb, Rt P, WB
50 µg / €350.00
  AVIVA Systems Biology

SLC12A1 antibody

Western Blot: SLC12A1 Antibody [NBP1-44269] - Western blot analysis of NKCC2 in rat kidney tissue using a 1:1000 dilution. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €450.00
  Novus Biologicals Inc.

SLC12A1 antibody

Immunohistochemistry-Paraffin: SLC12A1 Antibody [NBP1-80993] Immunohistochemical staining of human kidney shows strong cytoplasmic and membranous positivity in tubular cells. Rabbit IgG Aff - Purified Hu P
0.1 ml / €500.00
  Novus Biologicals Inc.

SLC12A1 antibody

Immunohistochemistry-Paraffin: SLC12A1 Antibody [NBP1-82559] - Immunohistochemical staining of human kidney shows strong membranous positivity in cells of distal tubules. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Western Blot/IP/ChiP Kits

Catalog No. Host Iso. Clone Pres. React. Applications  

SLC12A1 Western Blot Kit

SLC12A1 Western Blot Kit Hu
25 Western Blots / kit / €640.00
  AVIVA Systems Biology

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

SLC12A1

SLC12A1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

SLC12A1

SLC12A1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

SLC12A1

SLC12A1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

SLC12A1

SLC12A1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

16 Item(s)

per page
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