SLC12A2 / NKCC1 antibody

Principal name

SLC12A2 / NKCC1 antibody

Alternative names for SLC12A2 / NKCC1 antibody

Solute carrier family 12 member 2, Basolateral Na-K-Cl symporter, Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1

SwissProt ID

P55011 (Human), P55012 (Mouse)

Gene ID

6558 (SLC12A2)

Available reactivities

Bov (Bovine), Can (Canine), Hu (Human), Mky (Monkey), Ms (Mouse), Rt (Rat), Chimp (Chimpanzee), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Chicken, Rabbit, Goat, Mouse

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Frozen Sections (C), Dot blot (Dot)

Background of SLC12A2 / NKCC1 antibody

The -K-Cl cotransporters are a class of membrane proteins that transport , K, and Cl ions into and out of cells in an electrically neutral manner, in most cases with a stoichiometry of 1+:1K+:2Cl. -K-Cl cotransporters are present in a wide variety of cells and tissues, including reabsorptive and secretory epithelia, nerve and muscle cells, endothelial cells, fibroblasts, and blood cells. -K-Cl cotransport plays a vital role in rel salt reabsorption and in salt secretion by intestil, airway, salivary gland, and other secretory epithelia. -K-Cl cotransport activity is inhibited by "loop" diuretics, including the clinically efficacious agents, bumetanide and furosemide. The regulation of -K-Cl cotransport is mediated, at least in some cases, through direct phosphorylation of the cotransport protein. NKCC1 is one of two -K-Cl cotransporter isoforms that have been identified. NKCC1 is present in a wide variety of secretory epithelia and non-epithelial cells. NKCC1 is expressed in virtually all mammalian cells and is thought to play a housekeeping role in cell volume homeostasis and the common control of cytosolic ion content. NKCC1 does not use ATP but operates using electrochemical gradient for + and K+ produced by +-K+-ATPase. There is considerable evidence that uptake of Cl− in immature neurons is mediated by NKCC1. High expression of NKCC1 in immature neurons plays an important role in maintaining high intracellular Cl-.

General readings

1. Panet,R., Eliash,M. and Atlan,H., et al.
Na+/K+/Cl- cotransporter activates MAP-kinase cascade downstream to protein kinase C, and upstream to MEK
2. Dzhala,V.I., Talos,D.M., Sdrulla,D.A., Brumback,A.C., Mathews,G.C., Benke,T.A., Delpire,E., Jensen,F.E. and Staley,K.J.
NKCC1 transporter facilitates seizures in the developing brain
3. Liedtke,C.M., Wang,X. and Smallwood,N.D., et al.
Role for protein phosphatase 2A in the regulation of Calu-3 epithelial Na+-K+-2Cl-, type 1 co-transport function
4. Simard,C.F., Daigle,N.D., Bergeron,M.J., Brunet,G.M., Caron,L., Noel,M., Montminy,V. and Isenring,P.
Characterization of a novel interaction between the secretory Na+-K+-Cl- cotransporter and the chaperone hsp90
5. Simard,C.F., Brunet,G.M., Daigle,N.D., Montminy,V., Caron,L. and Isenring,P.
Self-interacting domains in the C terminus of a cation-Cl- cotransporter described for the first time
6. , Payne J.A., Xu J.-C., Haas M., Lytle Y.C., Ward D., Forbush B. III
Primary structure, functional expression, and chromosomal localization of the bumetanide-sensitive Na-K-Cl cotransporter in human colon.
7. , Vibat C.R., Holland M.J., Kang J.J., Putney L.K., O'Donnell M.E.
Quantitation of Na(+)-K(+)-2Cl(-) cotransport splice variants in human tissues using kinetic polymerase chain reaction.

19 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SLC12A2 / NKCC1 antibody

Western blot analysis of rat brain membranes (lanes 1 and 4), mouse brain membranes (lanes 2 and 5) and rat lung lysate (lanes 3 and 6): 1-3. Anti-NKCC1 (extracellular) antibody, (1:200). 4-6. Anti-NKCC1 (extracellular) antibody, preincubated with the control peptide antigen. Rabbit Purified Hu, Ms, Rt WB
0.2 ml / €435.00
  OriGene Technologies, Inc.

SLC12A2 / NKCC1 antibody

WB Suggested Anti-SLC12A2 Antibody Titration: 5.0 ug/ml; ELISA Titer: 1:1562500; Positive Control: DLD1 cell lysateSLC12A2 is supported by BioGPS gene expression data to be expressed in DLD1 Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SLC12A2 / NKCC1 antibody

Anti-SLC12A2 / NKCC1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Purified Hu
50 µg / €379.00
  OriGene Technologies, Inc.

SLC12A2 / NKCC1 antibody

Anti-SLC12A2 / NKCC1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Purified Hu
50 µg / €379.00
  OriGene Technologies, Inc.

SLC12A2 / NKCC1 antibody

Anti-SLC12A2 / NKCC1 antibody IHC of human prostate. human. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Purified Hu
50 µg / €379.00
  OriGene Technologies, Inc.

SLC12A2 / NKCC1 antibody

Human, Prostate: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Purified Hu
50 µg / €379.00
  OriGene Technologies, Inc.

SLC12A2 / NKCC1 antibody

Serial dilutions of BSA-conjugated modified and unmodified peptides dotted onto membrane and probed with N161/20 TC supe (left) and a mouse loading control (right). Mouse IgG1 N161/20 Supernatant Hu, Ms, Rt Dot
5 ml / €320.00
  Antibodies Incorporated

NeuroMab

SLC12A2 / NKCC1 antibody

Serial dilutions of BSA-conjugated modified and unmodified peptides dotted onto membrane and probed with N161/20 TC supe (left) and a mouse loading control (right). Mouse IgG1 N161/20 Purified Hu, Ms, Rt Dot
0.1 ml / €380.00
  Antibodies Incorporated

NeuroMab

SLC12A2 / NKCC1 antibody

Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2b 5H7 Purified Hu E, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

SLC12A2 / NKCC1 (769-783) antibody

Human Colon, Epithelium: Formalin-Fixed, Paraffin-Embedded (FFPE) Goat Aff - Purified Bear, Bov, Bt, Can, Chk, Elephant, Eq, Ft, Hst, Hu, Mky, Ms, Op, Por, Prim, Rt, Tr, Xen E, P
50 µg / €470.00
  Acris Antibodies GmbH

SLC12A2 / NKCC1 antibody

SLC12A2 / NKCC1 Rabbit Purified Hu C, E, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

SLC12A2 / NKCC1 antibody

SLC12A2 / NKCC1 Mouse IgG2b 5H7 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SLC12A2 / NKCC1 antibody

Human DLD1; WB Suggested Anti-SLC12A2 Antibody Titration: 5.0ug/ml. ELISA Titer: 1:1562500. Positive Control: DLD1 cell lysate. SLC12A2 is supported by BioGPS gene expression data to be expressed in DLD1.; SLC12A2 antibody - C-terminal region (ARP43805_T100) in Human DLD1 cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt WB
0.1 mg / €310.00
  AVIVA Systems Biology

SLC12A2 / NKCC1 antibody

Western Blot: E coli-derived fusion protein as test antigen. Affinity-purified IgY dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development. ( anti-SLC12A2 ) Chicken Aff - Purified Bov, Can, Hu, Mky, Ms, Rt WB
50 µg / €520.00
  Novus Biologicals Inc.

SLC12A2 / NKCC1 antibody

Immunohistochemistry-Paraffin: NKCC1 Antibody [NBP1-52148] - (3.8ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Goat Aff - Purified Hu E, P, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

SLC12A2 / NKCC1 antibody

Western Blot: NKCC1 Antibody [NBP1-59430] - This Anti-SLC12A2 antibody was used in Western Blot of DLD1 lysate at a concentration of 5ug/ml. Rabbit Purified Bov, Can, Hu, Ms, Rt WB
0.1 mg / €360.00
  Novus Biologicals Inc.

SLC12A2 / NKCC1 antibody

SLC12A2 / NKCC1 Rabbit Purified Hu, Ms, Rt P, WB
0.1 ml / €400.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

SLC12A2 / NKCC1

SLC12A2 / NKCC1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

SLC12A2 / NKCC1

SLC12A2 / NKCC1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

19 Item(s)

per page
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