SLC12A2 / NKCC1 antibody

Principal name

SLC12A2 / NKCC1 antibody

Alternative names for SLC12A2 / NKCC1 antibody

Solute carrier family 12 member 2, Basolateral Na-K-Cl symporter, Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1

SwissProt ID

P55011 (Human), P55012 (Mouse)

Gene ID

6558 (SLC12A2), 20496 (Slc12a2)

Available reactivities

Bov (Bovine), Can (Canine), Hu (Human), Ms (Mouse), Rt (Rat), Bt (Bat), Chk (Chicken), Eq (Equine), Ft (Ferret), Hst (Hamster), Mky (Monkey), Op (Opossum), Por (Porcine), Prim (Primate), Xen (Xenopus), Tr (Turkey), Bear, GP (Guinea Pig), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Rabbit, Goat, Mouse

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Frozen Sections (C), Dot blot (Dot)

Background of SLC12A2 / NKCC1 antibody

The -K-Cl cotransporters are a class of membrane proteins that transport , K, and Cl ions into and out of cells in an electrically neutral manner, in most cases with a stoichiometry of 1+:1K+:2Cl. -K-Cl cotransporters are present in a wide variety of cells and tissues, including reabsorptive and secretory epithelia, nerve and muscle cells, endothelial cells, fibroblasts, and blood cells. -K-Cl cotransport plays a vital role in rel salt reabsorption and in salt secretion by intestil, airway, salivary gland, and other secretory epithelia. -K-Cl cotransport activity is inhibited by "loop" diuretics, including the clinically efficacious agents, bumetanide and furosemide. The regulation of -K-Cl cotransport is mediated, at least in some cases, through direct phosphorylation of the cotransport protein. NKCC1 is one of two -K-Cl cotransporter isoforms that have been identified. NKCC1 is present in a wide variety of secretory epithelia and non-epithelial cells. NKCC1 is expressed in virtually all mammalian cells and is thought to play a housekeeping role in cell volume homeostasis and the common control of cytosolic ion content. NKCC1 does not use ATP but operates using electrochemical gradient for + and K+ produced by +-K+-ATPase. There is considerable evidence that uptake of Cl− in immature neurons is mediated by NKCC1. High expression of NKCC1 in immature neurons plays an important role in maintaining high intracellular Cl-.

General readings

Hannemann A, Flatman PW. Phosphorylation and transport in the Na-K-2Cl cotransporters, NKCC1 and NKCC2A, compared in HEK-293 cells. PLoS One. 2011 Mar 25;6(3):e17992.

19 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SLC12A2 / NKCC1 antibody

  Rabbit Polyclonal Anti-NKCC1 (extracellular)  
Western blot analysis of rat brain membranes (lanes 1 and 4), mouse brain membranes (lanes 2 and 5) and rat lung lysate (lanes 3 and 6): 1-3. Anti-NKCC1 (extracellular) antibody, (1:200). 4-6. Anti-NKCC1 (extracellular) antibody, preincubated with the control peptide antigen. Rabbit Purified Hu, Ms, Rt WB
0.2 ml / €435.00
  OriGene Technologies, Inc.

SLC12A2 / NKCC1 antibody

  Rabbit Polyclonal Anti-SLC12A2 Antibody  
WB Suggested Anti-SLC12A2 Antibody Titration: 5.0 ug/ml; ELISA Titer: 1:1562500; Positive Control: DLD1 cell lysateSLC12A2 is supported by BioGPS gene expression data to be expressed in DLD1 Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SLC12A2 / NKCC1 antibody

  Rabbit Polyclonal Anti-SLC12A2 Antibody (N-Terminus)  
Anti-SLC12A2 / NKCC1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Purified Hu
50 µg / €400.00
  OriGene Technologies, Inc.

SLC12A2 / NKCC1 antibody

  Rabbit Polyclonal Anti-SLC12A2 Antibody (Internal)  
Anti-SLC12A2 / NKCC1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Purified Hu
50 µg / €400.00
  OriGene Technologies, Inc.

SLC12A2 / NKCC1 antibody

  Rabbit Polyclonal Anti-SLC12A2 Antibody (C-Terminus)  
Anti-SLC12A2 / NKCC1 antibody IHC of human prostate. human. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Purified Hu
50 µg / €400.00
  OriGene Technologies, Inc.

SLC12A2 / NKCC1 antibody

  Rabbit Polyclonal Anti-SLC12A2 Antibody (Internal)  
Human, Prostate: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Purified Hu
50 µg / €400.00
  OriGene Technologies, Inc.

SLC12A2 / NKCC1 antibody

Serial dilutions of BSA-conjugated modified and unmodified peptides dotted onto membrane and probed with N161/20 TC supe (left) and a mouse loading control (right). Mouse IgG1 N161/20 Supernatant Hu, Ms, Rt Dot
5 ml / €320.00
  Antibodies Incorporated

NeuroMab

SLC12A2 / NKCC1 antibody

Serial dilutions of BSA-conjugated modified and unmodified peptides dotted onto membrane and probed with N161/20 TC supe (left) and a mouse loading control (right). Mouse IgG1 N161/20 Purified Hu, Ms, Rt Dot
0.1 ml / €380.00
  Antibodies Incorporated

NeuroMab

SLC12A2 / NKCC1 antibody

Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2b 5H7 Purified Hu E, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

SLC12A2 / NKCC1 (769-783) antibody

Human Colon, Epithelium: Formalin-Fixed, Paraffin-Embedded (FFPE) Goat Aff - Purified Bear, Bov, Bt, Can, Chk, Elephant, Eq, Ft, Hst, Hu, Mky, Ms, Op, Por, Prim, Rt, Tr, Xen E, P
50 µg / €470.00
  Acris Antibodies GmbH

SLC12A2 / NKCC1 antibody

SLC12A2 / NKCC1 Rabbit Purified Hu C, E, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

SLC12A2 - human gene knockout kit via CRISPR

SLC12A2 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

SLC12A2 - human gene knockout kit via CRISPR

SLC12A2 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

SLC12A2 - human gene knockout kit via CRISPR

SLC12A2 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

SLC12A2 - human gene knockout kit via CRISPR

SLC12A2 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, SLC12A2 (mGFP-tagged) - Human solute carrier family 12 (sodium/potassium/chloride transporters), member 2 (SLC12A2), 200 uL, >10^7 TU/mL

Lenti ORF particles, SLC12A2 (mGFP-tagged) - Human solute carrier family 12 (sodium/potassium/chloride transporters), member 2 (SLC12A2), 200 uL, >10^7 TU/mL  
200 µl / €2,370.00
  OriGene Technologies, Inc.

Lenti ORF particles, SLC12A2 (Myc-DDK tagged) - Human solute carrier family 12 (sodium/potassium/chloride transporters), member 2 (SLC12A2) , 200 uL, >10^7 TU/mL

Lenti ORF particles, SLC12A2 (Myc-DDK tagged) - Human solute carrier family 12 (sodium/potassium/chloride transporters), member 2 (SLC12A2) , 200 uL, >10^7 TU/mL  
200 µl / €2,140.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Slc12a2 (GFP-tagged) - Mouse solute carrier family 12, member 2 (Slc12a2), 200 uL, >10^7 TU/mL

Lenti ORF particles, Slc12a2 (GFP-tagged) - Mouse solute carrier family 12, member 2 (Slc12a2), 200 uL, >10^7 TU/mL  
200 µl / €2,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, Slc12a2 (Myc-DDK-tagged) - Mouse solute carrier family 12, member 2 (Slc12a2), 200 uL, >10^7 TU/mL

Lenti ORF particles, Slc12a2 (Myc-DDK-tagged) - Mouse solute carrier family 12, member 2 (Slc12a2), 200 uL, >10^7 TU/mL  
200 µl / €1,850.00
  OriGene Technologies, Inc.

19 Item(s)

per page
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