SLC18A3 / VACHT antibody

Principal name

SLC18A3 / VACHT antibody

Alternative names for SLC18A3 / VACHT antibody

VAChT, Vesicular acetylcholine transporter, Solute carrier family 18 member 3

SwissProt ID

O35304 (Mouse), P59845 (Danre), Q16572 (Human), Q62666 (Rat)

Gene ID

6572 (SLC18A3), 60422 (Slc18a3)

Available reactivities

Ms (Mouse), Rt (Rat), Hu (Human)

Available hosts

Goat, Rabbit, Guinea Pig, Mouse

Available applications

Immunoelectron Microscopy (EM), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Enzyme Immunoassay (E)

Background of SLC18A3 / VACHT antibody

In cholinergic neurons, acetylcholine is synthesized from choline and acetyl-coenzyme A (acetyl-CoA). Vesicular acetylcholine transporter (VAChT) transports acetylcholine from the cytoplasm to the lumen of syptic vesicles where following depolarization, acetylcholine is released in the syptic cleft and activates muscarinic and nicotinic receptors located on postsyptic membranes. Acetylcholine released in the syptic cleft is rapidly hydrolyzed into choline and acetate. Since choline is not de novo synthesized and thereby only made available through diet uptake, choline is recycled back into the cells in order to regenerate acetylcholine. VAChT is a member of the SLC18 family of transporters. It has 12 membrane spanning domains, with extracellular N- and C-termil tails. The transporter exchanges cytoplasmic acetylcholine for two vesicular protons. VAChT is a selective marker of cholinergic neurons and could be used therefore to determine the loss of cholinergic neurons in Alzheimerâ??s or Alzheimerâ??s related disease. VAChT knockout mice die after birth. They display a lack of stimulated release of acetylcholine and an underdeveloped neuromuscular junction.

17 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SLC18A3 / VACHT antibody

  Anti-SLC18A3 Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm. Positive control: Hela and HepG2 cell. Recommended dilution: 1/50-200 The image is immunocytochemistry of methanol-fixed Hela cell using SLC18A3 antibody at dilution 1/50, the secondary antibody is FITC conjugated Goat Anti-Rabbit IgG at dilution 1/200, blue is nucleus staining by Dapi. (Original magnification:×200) Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF
0.1 ml / €325.00
  OriGene Technologies, Inc.

SLC18A3 / VACHT (521-532) antibody

  Mouse monoclonal VAChT Antibody  
Western blot analysis of VAChT on rat membrane tissues using a 1:1000 dilution of the antibody Mouse IgG1 S6-38 Purified Hu, Ms, Rt C, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SLC18A3 / VACHT antibody

  Rabbit Polyclonal Anti-Vesicular Acetylcholine Transporter  
Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates: 1-2. Anti-Vesicular Acetylcholine Transporter antibody, (1:200). 3-4. Anti-Vesicular Acetylcholine Transporter antibody, preincubated with the control peptide antigen. Rabbit Purified Ms, Rt WB
0.2 ml / €435.00
  OriGene Technologies, Inc.

SLC18A3 / VACHT antibody

Adult rat brain immunohistochemistry Mouse IgG1 N6/38 Supernatant Hu, Ms, Rt C
5 ml / €320.00
  Antibodies Incorporated

NeuroMab

SLC18A3 / VACHT antibody

Adult rat brain immunohistochemistry Mouse IgG1 N6/38 Purified Hu, Ms, Rt C
0.1 ml / €380.00
  Antibodies Incorporated

NeuroMab

SLC18A3 / VACHT antibody

SLC18A3 / VACHT Rabbit Serum Hu C
50 µl / €580.00
  Acris Antibodies GmbH

SLC18A3 / VACHT antibody

SLC18A3 / VACHT Guinea Pig Serum Ms, Rt C, ICC/IF
50 µl / €580.00
  Acris Antibodies GmbH

SLC18A3 / VACHT antibody

IHC image of neurons in the rat basal forebrain staining for VAT. The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% rabbit serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:5000 for 48 hours, rabbit anti-goat secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen. Goat Serum Ms, Rt C, EM, ICC/IF
0.1 ml / €520.00
  ImmunoStar Inc.

CRISPR

Catalog No. Species Donor Vector  

SLC18A3 - human gene knockout kit via CRISPR

SLC18A3 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

SLC18A3 - human gene knockout kit via CRISPR

SLC18A3 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

SLC18A3 - human gene knockout kit via CRISPR

SLC18A3 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

SLC18A3 - human gene knockout kit via CRISPR

SLC18A3 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

SLC18A3 overexpression lysate

SLC18A3 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, SLC18A3 (mGFP-tagged) - Human solute carrier family 18 (vesicular acetylcholine), member 3 (SLC18A3), 200 uL, >10^7 TU/mL

Lenti ORF particles, SLC18A3 (mGFP-tagged) - Human solute carrier family 18 (vesicular acetylcholine), member 3 (SLC18A3), 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, SLC18A3 (Myc-DDK tagged) - Human solute carrier family 18 (vesicular acetylcholine), member 3 (SLC18A3) , 200 uL, >10^7 TU/mL

Lenti ORF particles, SLC18A3 (Myc-DDK tagged) - Human solute carrier family 18 (vesicular acetylcholine), member 3 (SLC18A3) , 200 uL, >10^7 TU/mL  
200 µl / €940.00
  OriGene Technologies, Inc.

Rat Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Slc18a3 (GFP-tagged ORF) - Rat solute carrier family 18 (vesicular acetylcholine), member 3 (Slc18a3), 200 uL, >10^7 TU/mL

Lenti ORF particles, Slc18a3 (GFP-tagged ORF) - Rat solute carrier family 18 (vesicular acetylcholine), member 3 (Slc18a3), 200 uL, >10^7 TU/mL  
200 µl / €1,160.00
  OriGene Technologies, Inc.

Lenti ORF particles, Slc18a3 (Myc-DDK-tagged ORF) - Rat solute carrier family 18 (vesicular acetylcholine), member 3 (Slc18a3), 200 uL, >10^7 TU/mL

Lenti ORF particles, Slc18a3 (Myc-DDK-tagged ORF) - Rat solute carrier family 18 (vesicular acetylcholine), member 3 (Slc18a3), 200 uL, >10^7 TU/mL  
200 µl / €1,037.00
  OriGene Technologies, Inc.

17 Item(s)

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