SLC18A3 / VACHT antibody

Principal name

SLC18A3 / VACHT antibody

Alternative names for SLC18A3 / VACHT antibody

VAChT, Vesicular acetylcholine transporter, Solute carrier family 18 member 3

SwissProt ID

O35304 (Mouse), P59845 (Danre), Q16572 (Human), Q62666 (Rat)

Gene ID

6572 (SLC18A3), 60422 (Slc18a3)

Available reactivities

Ms (Mouse), Rt (Rat), Hu (Human), GP (Guinea Pig)

Available hosts

Goat, Rabbit, Guinea Pig, Sheep, Mouse

Available applications

Immunoelectron Microscopy (EM), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Western blot / Immunoblot (WB)

Background of SLC18A3 / VACHT antibody

In cholinergic neurons, acetylcholine is synthesized from choline and acetyl-coenzyme A (acetyl-CoA). Vesicular acetylcholine transporter (VAChT) transports acetylcholine from the cytoplasm to the lumen of syptic vesicles where following depolarization, acetylcholine is released in the syptic cleft and activates muscarinic and nicotinic receptors located on postsyptic membranes. Acetylcholine released in the syptic cleft is rapidly hydrolyzed into choline and acetate. Since choline is not de novo synthesized and thereby only made available through diet uptake, choline is recycled back into the cells in order to regenerate acetylcholine. VAChT is a member of the SLC18 family of transporters. It has 12 membrane spanning domains, with extracellular N- and C-termil tails. The transporter exchanges cytoplasmic acetylcholine for two vesicular protons. VAChT is a selective marker of cholinergic neurons and could be used therefore to determine the loss of cholinergic neurons in Alzheimerâ??s or Alzheimerâ??s related disease. VAChT knockout mice die after birth. They display a lack of stimulated release of acetylcholine and an underdeveloped neuromuscular junction.

General readings

Bravo, D.T, er al. Biochemistry 44(22), 7955-7966 (2005)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SLC18A3 / VACHT antibody

Immunohistochemistry-Paraffin: Vesicular Acetylcholine Transporter Antibody [NB100-91348] - IHC on rat spinal cord (paraffin section) using Rabbit antibody to (Vesicular Acetylcholine Transporter) VACHT: whole serum at 1 : 200 dilution incubated overnight at 4C, developed with DAB/Ni and counterstained with hematoxylin. Rabbit Serum Hu, Ms, Rt C, P, WB
0.1 ml / €400.00
  Novus Biologicals Inc.

SLC18A3 / VACHT antibody

Predicted cell location: Cytoplasm. Positive control: Hela and HepG2 cell. Recommended dilution: 1/50-200 The image is immunocytochemistry of methanol-fixed Hela cell using SLC18A3 antibody at dilution 1/50, the secondary antibody is FITC conjugated Goat Anti-Rabbit IgG at dilution 1/200, blue is nucleus staining by Dapi. (Original magnification:×200) Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF
0.1 ml / €325.00
  OriGene Technologies, Inc.

SLC18A3 / VACHT (521-532) antibody

Western blot analysis of VAChT on rat membrane tissues using a 1:1000 dilution of the antibody Mouse IgG1 S6-38 Purified Hu, Ms, Rt C, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SLC18A3 / VACHT antibody

Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates: 1-2. Anti-Vesicular Acetylcholine Transporter antibody, (1:200). 3-4. Anti-Vesicular Acetylcholine Transporter antibody, preincubated with the control peptide antigen. Rabbit Purified Ms, Rt WB
0.2 ml / €435.00
  OriGene Technologies, Inc.

SLC18A3 / VACHT antibody

Adult rat brain immunohistochemistry Mouse IgG1 N6/38 Supernatant Hu, Ms, Rt C
5 ml / €320.00
  Antibodies Incorporated

NeuroMab

SLC18A3 / VACHT antibody

Adult rat brain immunohistochemistry Mouse IgG1 N6/38 Purified Hu, Ms, Rt C
0.1 ml / €380.00
  Antibodies Incorporated

NeuroMab

SLC18A3 / VACHT antibody

SLC18A3 / VACHT Rabbit Serum Hu C
50 µl / €560.00
  Acris Antibodies GmbH

SLC18A3 / VACHT antibody

SLC18A3 / VACHT Guinea Pig Serum Ms, Rt C, ICC/IF
50 µl / €560.00
  Acris Antibodies GmbH

SLC18A3 / VACHT antibody

SLC18A3 / VACHT Mouse Hu WB
0.5 mg / €1,400.00
  Abnova Taiwan Corp.

SLC18A3 / VACHT antibody

IHC image of neurons in the rat basal forebrain staining for VAT. The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% rabbit serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:5000 for 48 hours, rabbit anti-goat secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen. Goat Serum Ms, Rt C, EM, ICC/IF
0.1 ml / €490.00
  ImmunoStar Inc.

SLC18A3 / VACHT antibody

SLC18A3 / VACHT Rabbit Aff - Purified GP, Rt P, WB
20 µg / €480.00
  Novus Biologicals Inc.

SLC18A3 / VACHT antibody

SLC18A3 / VACHT Sheep Aff - Purified GP, Rt ICC/IF, P
20 µg / €480.00
  Novus Biologicals Inc.

SLC18A3 / VACHT antibody

SLC18A3 / VACHT Goat Serum Hu, Ms, Rt P, WB
0.1 ml / €400.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

SLC18A3 / VACHT

SLC18A3 / VACHT Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

SLC18A3 / VACHT

SLC18A3 / VACHT Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

SLC18A3 overexpression lysate

SLC18A3 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

16 Item(s)

per page
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