SLC1A2 / EAAT2 antibody

Principal name

SLC1A2 / EAAT2 antibody

Alternative names for SLC1A2 / EAAT2 antibody

GLT1, Solute carrier family 1 member 2, Excitatory amino acid transporter 2, Sodium-dependent glutamate/aspartate transporter 2, Glutamate/aspartate transporter II

SwissProt ID

P31596 (Rat), P43004 (Human), P43006 (Mouse)

Gene ID

6506 (SLC1A2), 29482 (Slc1a2)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Chk (Chicken), Ze (Zebrafish), Bov (Bovine), Eq (Equine), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), ELISA (detection) (E(detection)), Frozen Sections (C)

Background of SLC1A2 / EAAT2 antibody

L-Glutamate (Glu) is an abundant amino acid that functions as the major excitatory neurotransmitter in the central nervous system. However, excess of Glu in the extracellular syptic milieu leads to neurol cell death by a process known as excitotoxicity. The extracellular levels of Glu are regulated by a family of high affinity plasma membrane transporters called excitatory amino acid transporters (EAATs) which are responsible for the re-uptake of Glu into the cells. The EAAT family includes five members (EAAT1-EAAT5) that are members of the solute carrier family 1 (SLC1) of +-dependent transporters that also includes the neutral amino acid transporters ASCT1 and ASCT2. The Glu transporters present an unusual topology of eight transmembrane domains with two re-entrant loops and intracellular N- and C- termini. The transporter is likely assembled as a trimer where each monomer is a functiol unit capable of binding the Glu substrate. The transport of Glu into the cells by the EAAT transporters is coupled to the + and K+ electrochemical gradient as a driving force. Hence, the uptake of Glu is dependent on the co-transport of three + and one H+ ions, and the counter transport of one K+ ion. In addition, to the well documented Glu uptake, the EAAT transporters show a Glu-independent Cl conductance. The physiological significance of the Cl current through the EAATs is currently unknown. EAAT2 (also known as Glutamate Transporter-1, GLT-1) as well as EAAT1, is expressed predomintly in glia cells, while EAAT3, EAAT4 and EAAT5 are mostly expressed in neurons. As mentioned earlier, EAAT transporters represent the only (significant) mechanism for removal of glutamate from the extracellular fluid and hence are essential for the long-term maintence of low and non-toxic concentrations of glutamate and the preservation of normal excitatory syptic transmission. In addition to Glu uptake, the glutamate transporters provide glutamate for the synthesis of g-Aminobutyric acid (GABA), glutathione and protein, suggesting an interactive role between EAATs and cellular metabolism. Dysregulation of EAAT activities has been implicated in several neurodegenerative disorders such as Alzheimerâ??s disease, traumatic brain injury, epilepsy and schizophrenia, suggesting that EAATs can be a useful target for the treatment of these conditions.

General readings

1. Lauriat TL, McInnes LA. EAAT2 regulation and splicing: relevance to psychiatric and neurological disorders. Mol Psychiatry. (2007 Aug 7) [Epub ahead of print]
2. Berger UV, DeSilva TM, Chen W, Rosenberg PA. Cellular and subcellular mRNA localization of glutamate transporter isoforms GLT1a and GLT1b in rat brain by in situ hybridization. J Comp Neurol. 492(1):78-89 (2005)
3. Yi JH, Pow DV, Hazell AS. Early loss of the glutamate transporter splice-variant GLT-1v in rat cerebral cortex following lateral fluid-percussion injury. Glia. 49(1):121-33 (2005)
4. Pow DV, Naidoo T, Lingwood BE, Healy GN, Williams SM, Sullivan RK, O'Driscoll S, Colditz PB. Loss of glial glutamate transporters and induction of neuronal expression of GLT-1B in the hypoxic neonatal pig brain. Brain Res Dev Brain Res. 153(1):1-11(2004)
5. Maragakis NJ, Dykes-Hoberg M, Rothstein JD. Altered expression of the glutamate transporter EAAT2b in neurological disease. Ann Neurol. 55(4):469-77 (2004)
6. Chen W, Aoki C, Mahadomrongkul V, Gruber CE, Wang GJ, Blitzblau R, Irwin N, Rosenberg PA. Expression of a variant form of the glutamate transporter GLT1 in neuronal cultures and in neurons and astrocytes in the rat brain. J Neurosci. 22(6):2142-52 (2002)
7. Schmitt A, Asan E, Lesch KP, Kugler P. A splice variant of glutamate transporter GLT1/EAAT2 expressed in neurons: cloning and localization in rat nervous system.
Neuroscience. 109(1):45-61 (2002)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SLC1A2 / EAAT2 antibody

Immunoreactivity of this antibody on protein extract from rat (lane1) and mouse brain (lane2).
GLT1 protein is detected at 35 kDa and 70 kDa as dimmer
In lane 3, inhibition of the immunoreactivity of the Ab with blocking peptide (1/1000 dilution) Rabbit Serum Rt C, E, WB
0.2 ml / €400.00
  Acris Antibodies GmbH

SLC1A2 / EAAT2 antibody

WB Suggested Anti-SLC1A2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human brain Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rb, Rt P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

SLC1A2 / EAAT2 antibody

Human Lung; . Rabbit Anti-SLC1A2 Antibody. . Catalog Number:. ARP33840 . Paraffin Embedded Tissue: Human alveolar cell . Cellular Data: Epithelial cells of renal tubule. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X Rabbit Aff - Purified Can, Chk, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

SLC1A2 / EAAT2 antibody

Predicted cell location: Cytoplasm, Cell membrane. Positive control: Human brain and liver cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using SLC1A2 antibody at dilution 1/20, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

SLC1A2 / EAAT2 antibody

Western blot analysis of rat cerebellum lysate: 1. Anti-Excitatory Amino Acid Transporter 2 (extracellular) antibody, (1:500). 2. Anti-Excitatory Amino Acid Transporter 2 (extracellular) antibody, preincubated with the control peptide antigen. Rabbit Purified Hu, Ms, Rt WB
0.2 ml / €435.00
  OriGene Technologies, Inc.

SLC1A2 / EAAT2 antibody

WB Suggested Anti-SLC1A2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:12500; Positive Control: HT1080 cell lysate Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rb, Rt P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

SLC1A2 / EAAT2 antibody

SLC1A2 / EAAT2 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

SLC1A2 / EAAT2 antibody

Western Blot analysis of SLC1A2 expression in transfected 293T cell line (H00006506-T02) by SLC1A2 MaxPab polyclonal antibody.

Lane 1: SLC1A2 transfected lysate(62.10 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

SLC1A2 / EAAT2 antibody

SLC1A2 monoclonal antibody (M07), clone 1D8. Western Blot analysis of SLC1A2 expression in HeLa ( Cat # L013V1 ). Mouse IgG2a 1D8 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SLC1A2 / EAAT2 antibody

Detection limit for recombinant GST tagged SLC1A2 is approximately 0.3ng/ml as a capture antibody. Mouse IgG2a 4G8 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SLC1A2 / EAAT2 antibody

Human brain; Brain, cortex; SLC1A2 antibody - middle region (ARP33841_P050) in Human brain cells using Immunohistochemistry Rabbit Aff - Purified Can, Chk, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

SLC1A2 / EAAT2 antibody

Western blot analysis of EAAT2 expression in mouse brain (A); rat brain (B) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

SLC1A2 / EAAT2 antibody

Western blot analysis of EAAT2 expression in mouse brain (A); rat brain (B) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

SLC1A2 / EAAT2 antibody

Western blot analysis of EAAT2 expression in mouse brain (A); rat brain (B) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

SLC1A2 / EAAT2 antibody

Immunocytochemistry: GLT1b Antibody (10B7) [NB110-58775] - Immunocytochemistry on COS7 cells transfected with GLT1b using GLT1b monoclonal antibody. Mouse IgG2a 10B7 Supernatant Ms, Rt ICC/IF, P, WB
0.1 ml / €360.00
  Novus Biologicals Inc.

SLC1A2 / EAAT2 antibody

SLC1A2 / EAAT2 Mouse IgG2a 10B7 Supernatant Ms, Rt ICC/IF, P, WB
25 µl / €160.00
  Novus Biologicals Inc.

SLC1A2 / EAAT2 (GLT1b) antibody

SLC1A2 / EAAT2 Rabbit Serum Hu, Ms, Rt P, WB
0.1 ml / €400.00
  Novus Biologicals Inc.

SLC1A2 / EAAT2 antibody

Western Blot: GLT1 Antibody [NBP1-59632] - This Anti-SLC1A2 antibody was used in Western Blot of Fetal Brain tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Can, Chk, Hu, Ms, Rt, Ze P, WB
50 µg / €440.00
  Novus Biologicals Inc.

SLC1A2 / EAAT2 antibody

Western Blot: GLT1 Antibody [NBP1-59633] - This Anti-SLC1A2 antibody was used in Western Blot of HT1080     tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu, Ms, Rt P, WB
50 µg / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

SLC1A2 / EAAT2

SLC1A2 / EAAT2 Human Purified
2 µg / €300.00
  Abnova Taiwan Corp.

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