SLC1A3 / EAAT1 antibody

Principal name

SLC1A3 / EAAT1 antibody

Alternative names for SLC1A3 / EAAT1 antibody

GLAST, GLAST1, GLAST-1, Solute carrier family 1 member 3, Excitatory amino acid transporter 1, Sodium-dependent glutamate/aspartate transporter 1

SwissProt ID

P24942 (Rat), P43003 (Human), P46411 (Bovin), P56564 (Mouse), Q10901 (Caeel)

Gene ID

29483 (Slc1a3), 6507 (SLC1A3)

Available reactivities

Rt (Rat), Hu (Human), Ms (Mouse), Bov (Bovine), Chk (Chicken), Eq (Equine), GP (Guinea Pig), Hst (Hamster), Mky (Monkey), Por (Porcine), Rb (Rabbit), Gorilla

Available hosts

Rabbit, Mouse, Goat

Available applications

Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Flow Cytometry (F), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Paraffin Sections (P)

Background of SLC1A3 / EAAT1 antibody

L-Glutamate (Glu) is an abundant amino acid that functions as the major excitatory neurotransmitter in the central nervous system. However, excess of Glu in the extracellular syptic milieu leads to neurol cell death by a process known as excitotoxicity. The extracellular levels of Glu are regulated by a family of high affinity plasma membrane transporters called excitatory amino acid transporters (EAATs) which are responsible for re-uptake of Glu into the cells. The EAAT family includes five members (EAAT1-EAAT5) that are members of the solute carrier family 1 (SLC1) of sodium-dependent transporters that also includes the neutral amino acid transporters ASCT1 and ASCT2. The Glu transporters present an unusual topology of eight transmembrane domains with two re-entrant loops and intracellular N- and C- termini. The transporter is likely assembled as a trimer where each monomer is a functiol unit capable of binding the Glu substrate. The transport of Glu into the cells by the EAAT transporters is coupled to the + and K+ electrochemical gradient as a driving force. Hence, the uptake of Glu is dependent on the co-transport of three + and one H+ ions, and the counter transport of one K+ ion. In addition, to the well documented Glu uptake, the EAAT transporters show a Glu-independent Cl conductance. The physiological significance of the Cl current through the EAATs is currently unknown. EAAT1 as well as EAAT2, is expressed predomintly in glia cells (hence its origil me: glial glutamate transporter or GLAST), while EAAT3, EAAT4 and EAAT5 are mostly expressed in neurons. As mentioned earlier, EAAT transporters represent the only (significant) mechanism for removal of glutamate from the extracellular fluid and hence are essential for the long-term maintence of low and non-toxic concentrations of glutamate and the preservation of normal excitatory syptic transmission. In addition, to Glu uptake the glutamate transporters provide glutamate for the synthesis of ?-Aminobutyric acid (GABA), glutathione and protein, suggesting an interactive role between EAATs and cellular metabolism. Dysregulation of EAATs activities has been implicated in several neurodegenerative disorders such as Alzheimerâ??s disease, traumatic brain injury, epilepsy and schizophrenia, suggesting that EAATs can be a useful target for the treatment of these conditions.

General readings

Kobayashi S & Millhorn DE Hypoxia regulates glutamate metabolism and membrane transport in rat PC12 cells. J Neurochem 76:1935-48 (2001). PubMed PMID: 11259512
General / background references:Schmitt A et al. Cellular and regional distribution of the glutamate transporter GLAST in the CNS of rats: nonradioactive in situ hybridization and comparative immunocytochemistry. J Neurosci 17:1-10 (1997). PubMed PMID: 8987731
Rothstein JD et al. Knockout of glutamate transporters reveals a major role for astroglial transport in excitotoxicity and clearance of glutamate. Neuron 16:675-86 (1996). PubMed PMID: 8785064

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SLC1A3 / EAAT1 antibody

Western Blot: SLC1A3 Antibody [TA336274] - Western blot analysis of human brain tissue (1), human brain membrane tissue (2), human hippocampus tissue (3), bovine brain (4), rat brain tissue (5), rat brain membrane tissue (6), mouse brain tissue (7), and m Rabbit Purified Hu, Ms, Rt C, E, F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SLC1A3 / EAAT1 (C-term) antibody

Staining of Human Hippocampus lysate using SLC1A3 / EAAT1 antibody Cat.-No AP32155PU-N at 2 μg/ml (35μg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu WB
0.1 mg / €340.00
  Acris Antibodies GmbH

SLC1A3 / EAAT1 (197-208) antibody

Staining of Human Frontal Cortex lysate using SLC1A3 / EAAT1 antibody Cat.-No AP32158PU-N at 0.1 μg/ml (35μg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu, Rt WB
0.1 mg / €340.00
  Acris Antibodies GmbH

SLC1A3 / EAAT1 antibody

Anti-SLC1A3 / GLAST antibody IHC of human brain, cerebellum, molecular layer and Purkinje layer. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit IgG Purified Bov, Chk, Eq, Gorilla, GP, Hst, Hu, Mky, Ms, Por, Rb, Rt
50 µg / €345.00
  OriGene Technologies, Inc.

SLC1A3 / EAAT1 antibody

Anti-SLC1A3 / GLAST antibody IHC of human brain, cerebellum, molecular layer and Purkinje layer. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit IgG Purified Eq, Gorilla, Hst, Hu, Mky, Ms
50 µg / €345.00
  OriGene Technologies, Inc.

SLC1A3 / EAAT1 antibody

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes: 1, 2. Anti-Excitatory Amino Acid Transporter 1 (extracellular) antibody (1:500). 3, 4. Anti-Excitatory Amino Acid Transporter 1 (extracellular) antibody, preincubated with the control peptide antigen. Rabbit Purified Ms, Rt WB
0.2 ml / €435.00
  OriGene Technologies, Inc.

SLC1A3 / EAAT1 antibody

Immunohistochemistry: SLC1A3 Antibody [TA336755] - IHC staining of SLC1A1 in mouse brain. Rabbit Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SLC1A3 / EAAT1 (C-term) antibody

Western blot analysis of rat pancreas tissue lysis using GLAST antibody Rabbit IgG Aff - Purified Hu, Ms, Rt WB
0.1 mg / €340.00
  Acris Antibodies GmbH

SLC1A3 / EAAT1 antibody

Frontal slices of rat brain cortex and rat brain cerebellum, respectively immunolabelled with anti-GLAST antibody, show a specific astrocytic labelling. Arrows show Glast-immunoreactivity on Bergmann gia (e, scale bar = 30 µm). Rabbit Serum Rt C, E, F, ICC/IF, WB
0.2 ml / €400.00
  Acris Antibodies GmbH

SLC1A3 / EAAT1 antibody

Western Blot analysis of SLC1A3 expression in transfected 293T cell line (H00006507-T02) by SLC1A3 MaxPab polyclonal antibody.

Lane 1: SLC1A3 transfected lysate(59.50 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

SLC1A3 / EAAT1 antibody

SLC1A3 / EAAT1 Mouse IgM 3D1 Ascites Hu E, WB
0.2 ml / €370.00
  Abnova Taiwan Corp.

SLC1A3 / EAAT1 antibody

SLC1A3 / EAAT1 Rabbit Serum Hu C, P, WB
0.1 ml / €400.00
  Novus Biologicals Inc.

SLC1A3 / EAAT1 antibody

Immunohistochemistry-Paraffin: SLC1A3 Antibody [NBP1-84939] Immunohistochemical staining of human cerebellum shows distinct positivity in neuropil. Rabbit IgG Aff - Purified Hu P
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

SLC1A3 / EAAT1

SLC1A3 / EAAT1 Human
2 µg / €300.00
  Abnova Taiwan Corp.

SLC1A3 / EAAT1

SLC1A3 / EAAT1 Human Purified
10 µg / €380.00
  Abnova Taiwan Corp.

SLC1A3 / EAAT1

SLC1A3 / EAAT1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue
10 µg / €380.00
  Abnova Taiwan Corp.

SLC1A3 / EAAT1

SLC1A3 / EAAT1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue
25 µg / €510.00
  Abnova Taiwan Corp.

SLC1A3 / EAAT1

SLC1A3 / EAAT1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

SLC1A3 / EAAT1

SLC1A3 / EAAT1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

19 Item(s)

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