SLC29A1 / ENT1 antibody

Principal name

SLC29A1 / ENT1 antibody

Alternative names for SLC29A1 / ENT1 antibody

Solute carrier family 29 member 1, Equilibrative nucleoside transporter 1

SwissProt ID

O54698 (Rat), Q99808 (Human), Q9JIM1 (Mouse)

Gene ID

2030 (SLC29A1), 23071 (ERP44)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Mky (Monkey), Por (Porcine)

Available hosts

Rabbit, Mouse, Goat

Available applications

Enzyme Immunoassay (E), Immunoprecipitation (IP), Paraffin Sections (P), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of SLC29A1 / ENT1 antibody

Nucleosides play other important roles beyond their nucleic acid synthesis building block role. For example, they are involved in energy metabolism; they serve as ligands of purinergic receptors and act as influential sigling molecules. Being hydrophilic, nucleosides cannot simply diffuse across the plasma membrane in order to exert their various functions, but rather need to be physically transported via nucleoside transporters. Two different transporter families are responsible for transporting nucleosides across the plasma membrane: The Concentrative Nucleoside Transporter proteins (CNT, SLC28 family), which consist of three members, CNT1-3, and act as +-dependent symporters. The Equilibrative Nucleoside Transporter proteins ENT1-4 (ENT, SLC29 family), which mediate a +-independent facilitated diffusion. Therefore, ENTs act as bidirectiol carriers, responsible for the influx and efflux of substrates. Structurally, ENT transporters have eleven transmembrane domains with an intracellular N-termil and an extracellular C-termil. The best characterized ENT transporters are ENT1 and ENT2, which although display a broader range of substrate selectivity, have lower affinities for nucleosides compared to concentrative transporters. They are ubiquitously expressed, for example ENT1 is expressed in erythrocytes, vascular endothelium, placenta, brain, heart, liver and colon. ENT2 displays more or less the same expression pattern but in addition, is strongly expressed in skeletal muscle. ENT3 is a lysosomal pH-dependent transporter capable of transporting adenine, and ENT4 also transports adenine at acidic pH. They are also broadly expressed with ENT3 displaying high expression in the placenta and ENT4 in the heart. As mentioned above, nucleosides have a variety of cellular/physiological functions suggesting that transporters responsible for their trafficking may also have functiol attributes. Indeed, ENT1 plays a role in proliferation and therefore is responsible for the constitutive trafficking of nucleosides. There is no evidence that nucleoside transporters are directly involved in pathophysiologies, but they are clinically significant. For example, nucleoside transporters are responsible for the cellular uptake of a number of nucleoside-derived anticancer drugs.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SLC29A1 / ENT1 antibody

Human Kidney (formalin-fixed, paraffin-embedded) stained with SLC29A1 antibody AP22746PU-N followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit Purified Hu E, P, WB
0.2 ml / €470.00
  Acris Antibodies GmbH

SLC29A1 / ENT1 (Center) antibody

ENT1(Slc29a1) (Center) (Cat.#TA302009) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the ENT1 antibody detected ENT1 protein (arrow). Rabbit Ig Aff - Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SLC29A1 / ENT1 antibody

Western blot analysis of rat kidney (lanes 1 and 3) lysates and rat brain (lanes 2 and 4) membranes: 1-2. Anti-Equilibrative Nucleoside Transporter 1 (ENT1) antibody, (1:200). 3-4. Anti-Equilibrative Nucleoside Transporter 1 (ENT1) antibody, preincubated with the control peptide antigen. Rabbit Purified Rt WB
0.2 ml / €435.00
  OriGene Technologies, Inc.

SLC29A1 / ENT1 (N-term) antibody

TA329089 (1µg/ml) staining of Human Placenta lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SLC29A1 / ENT1 antibody

Anti-ENT1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Purified Hu
50 µg / €379.00
  OriGene Technologies, Inc.

SLC29A1 / ENT1 antibody

Anti-ENT1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit IgG Aff - Purified Hu P
50 µg / €379.00
  OriGene Technologies, Inc.

SLC29A1 / ENT1 antibody

Anti-ENT1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit IgG Aff - Purified Hu P
50 µg / €379.00
  OriGene Technologies, Inc.

SLC29A1 / ENT1 (C-term) antibody

Anti-ENT1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Purified Hu P
50 µg / €379.00
  OriGene Technologies, Inc.

SLC29A1 / ENT1 antibody

Western blot analysis of SLC29A1 in human ovary tissue lysate with SLC29A1 antibody at (A) 1 and (B) 2 ug/mL. Rabbit IgG Purified Hu ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SLC29A1 / ENT1 (C-term) antibody

Western blot analysis of ENT1 Antibody (C-term) (Cat. #AP11116PU-N) in mouse heart tissue lysates (35ug/lane). ENT1 (arrow) was detected using the purified Pab (1:300 dilution). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

SLC29A1 / ENT1 antibody

Western blot analysis of anti-ENT1(Slc29a1) Antibody (S254) (Cat.#AP11118PU-N) in 293 cell line lysates (35ug/lane). ENT1 (arrow) was detected using the purified Pab. Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

SLC29A1 / ENT1 pSer254 antibody

Dot blot analysis of anti-Phospho-ENT1(Slc29a1)-pS254 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml. Rabbit Ig Aff - Purified Hu

Regular Price: 0.4 ml / €370.00

Special Price: 0.4 ml / €231.00

  Acris Antibodies GmbH

SLC29A1 / ENT1 antibody

Western Blot analysis of SLC29A1 expression in transfected 293T cell line (H00002030-T01) by SLC29A1 MaxPab polyclonal antibody.

Lane 1: SLC29A1 transfected lysate(50.16 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

SLC29A1 / ENT1 antibody

Western Blot analysis of SLC29A1 expression in transfected 293T cell line (H00002030-T01) by SLC29A1 MaxPab polyclonal antibody.

Lane 1: SLC29A1 transfected lysate(50.16 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

SLC29A1 / ENT1 antibody

Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with SLC29A1 polyclonal antibody ( Cat # PAB2255 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. Rabbit Hu, Ms E, P, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

SLC29A1 / ENT1 antibody

Western blot analysis of ENT1 expression in SKNSH (A); mouse heart (B); rat heart (C) whole cell lysates. Rabbit Aff - Purified Bov, Can, Hu, Mky, Ms, Rt WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

SLC29A1 / ENT1 antibody

Western blot analysis of ENT1 expression in SKNSH (A); mouse heart (B); rat heart (C) whole cell lysates. Rabbit Aff - Purified Bov, Can, Hu, Mky, Ms, Rt WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

SLC29A1 / ENT1 antibody

Western blot analysis of ENT1 expression in SKNSH (A); mouse heart (B); rat heart (C) whole cell lysates. Rabbit Aff - Purified Bov, Can, Hu, Mky, Ms, Rt WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

SLC29A1 / ENT1 antibody

Immunohistochemistry-Paraffin: ENT1 Antibody [NBP1-50508] - Human Kidney (formalin-fixed, paraffin-embedded) stained with SLC29A1 antibody followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit Aff - Purified Hu E, P, WB
50 µg / €480.00
  Novus Biologicals Inc.

SLC29A1 / ENT1 antibody

Immunohistochemistry-Paraffin: ENT1 Antibody [NBP1-84838] - Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

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