SLC5A7 / CHT1 antibody

Principal name

SLC5A7 / CHT1 antibody

Alternative names for SLC5A7 / CHT1 antibody

High affinity choline transporter 1, Hemicholinium-3-sensitive choline transporter, Solute carrier family 5 member 7

SwissProt ID

Q8BGY9 (Mouse), Q9GZV3 (Human), Q9JMD7 (Rat)

Gene ID

60482 (SLC5A7), 63993 (Slc5a7)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Ze (Zebrafish), Mky (Monkey)

Available hosts

Mouse, Rabbit

Available applications

Frozen Sections (C), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of SLC5A7 / CHT1 antibody

In cholinergic neurons, acetylcholine is synthesized from choline and acetyl-coenzyme A (acetyl-CoA). Following depolarization, acetylcholine is released in the syptic cleft via syptic vesicles and activates muscarinic and nicotinic receptors located on postsyptic membranes. Acetylcholine released in the syptic cleft is rapidly hydrolyzed into choline and acetate. Since choline is not de novo synthesized and thereby only made available through diet uptake, choline is recycled back into the cells in order to regenerate acetylcholine. For this purpose, choline is taken up by the high affinity choline transporter (CHT). CHT has thirteen transmembrane domains, an extracellular N-terminus and an intracellular C-terminus. CHT belongs to the +-dependent glucose transporter family (SLC5). The activity of the transporter can be confirmed by its sensitivity to hemicholimium-3 (HC-3) which inhibits the transport of choline with a Ki of 10-100 nM. The activity of CHT reflects that of neurons i.e., increased CHT activity indicates an increase in neurol activity. The increase in the activity of the transporter is due to the increase in the number of transporters and not due to the increase in activity per se. In addition CHT activity is also regulated by second messengers. Phosphorylation of the transporter also seems to determine the activity as it has many consensus phosphorylation sites present in the C-termil. Expression studies reveal that CHT is expressed solely in cholinergic neurons making the transporter a useful marker for detecting these types of neurons.

General readings

Ferguson, S.M. et al. J. of Neuroscience 23(30): 9697-9709 (2003)

11 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SLC5A7 / CHT1 antibody

Staining of neurons in rat basal forebrain Mouse IgG1 62-2E8 Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SLC5A7 / CHT1 antibody

Western blot analysis of human SH-5YSY neuroblastoma cell lysate (lanes 1 and 4), rat (lanes 2 and 5) and mouse (lanes 3 and 6) brain lysates: 1-3. Anti-Choline Transporter (CHT) (extracellular) antibody, (1:200). 4-6. Anti-Choline Transporter (CHT) (extracellular) antibody, preincubated with the control peptide antigen. Rabbit Purified Hu, Ms, Rt WB
0.2 ml / €435.00
  OriGene Technologies, Inc.

SLC5A7 / CHT1 antibody

WB Suggested Anti-SLC5A7 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: 721_B cell lysate Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

SLC5A7 / CHT1 antibody

Host: Rabbit; Target Name: SLC5A7; Sample Tissue: ACHN Whole Cell lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

SLC5A7 / CHT1 antibody

Immunohistochemical staining of SLC5A7 on rat basal forebrain with SLC5A7 monoclonal antibody, clone 62-2E8 ( Cat # MAB2295 ). Mouse IgG1 62-2E8 Hu, Ms, Rt C, P, WB
0.1 ml / €470.00
  Abnova Taiwan Corp.

SLC5A7 / CHT1 antibody

SLC5A7 / CHT1 Rabbit Hu, Ms, Rt E, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

SLC5A7 / CHT1 antibody

Human 721_B; WB Suggested Anti-SLC5A7 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: 721_B cell lysate; SLC5A7 antibody - middle region (ARP44024_P050) in Human 721_B cells using Western Blot Rabbit Aff - Purified Bov, Can, Chk, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

SLC5A7 / CHT1 antibody

SLC5A7 / CHT1 Mouse IgG1 62-2E8 Ascites Hu, Ms, Rt C, P, WB
25 µl / €170.00
  Novus Biologicals Inc.

SLC5A7 / CHT1 antibody

Western Blot: CHT1 Antibody [NBP1-62339] - This Anti-SLC5A7 antibody was used in Western Blot of 721_B tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €440.00
  Novus Biologicals Inc.

SLC5A7 / CHT1 antibody

SLC5A7 / CHT1 Mouse IgG1 62-2E8 Purified Hu, Mky, Ms, Rt C, P, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

Lysates

Catalog No.    

SLC5A7 overexpression lysate

SLC5A7 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

11 Item(s)

per page
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