SLC6A3 / DAT antibody

Principal name

SLC6A3 / DAT antibody

Alternative names for SLC6A3 / DAT antibody

Sodium-dependent dopamine transporter, DA transporter, DAT1, Solute carrier family 6 member 3

SwissProt ID

P23977 (Rat), P27922 (Bovin), Q01959 (Human), Q61327 (Mouse)

Gene ID

6531 (SLC6A3), 13162 (Slc6a3), 24898

Available reactivities

Hst (Hamster), Hu (Human), Mky (Monkey), Ms (Mouse), Rt (Rat), Prim (Primate), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Rabbit, Mouse, Rat, Goat

Available applications

Frozen Sections (C), Paraffin Sections (P), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Dot blot (Dot), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of SLC6A3 / DAT antibody

The +/Cl transporter family, SLC6, includes DAT a monoamine transporter, important for regulating extracellular levels of dopamine. It does so by uptaking dopamine from the syptic cleft via the co-transport of + and Cl down their electrochemical gradient. The removal of dopamine by DAT remains the most important means to control the extracellular lifetime of the neurotransmitter, and notably the ending of dopaminergic neurotransmission. Transporters for serotonin, norepinephrine, GABA and glycine also belong to this family. These receptors have 12 transmembrane spanning domains and intracellular N- and C-termil domains. DAT is also subjected to post translatiol modifications such phosphorylation, important for its regulation. Also, it possesses a large extracellular domain which undergoes N-glycosylation, important for the proper targeting of the transporter to the plasma membrane. DAT is expressed in dopaminergic cell bodies and termils and can therefore serve as a marker for these neurons. DAT is also expressed in the reti, gastrointestil tract, lung, kidney, pancreas and lymphocytes. DAT plays an important role in movement as well as reward, learning and memory. Its malfunction which leads to dopaminergic dysregulation has been associated with ADHD, schizophrenia, as well as Parkinsonâ??s disease. DAT is the main target for cocaine and amphetamine and methamphetamine psychostimulants, which mainly increase locomotor activities.

General readings

1. Miller, G.W. et al. (1997) Immunochemical analysis of dopamine transporter protein in Parkinson's disease. Ann Neurol. 41:530-539.
2. Kwon, O.B. et al (2008) Neuregulin-1 regulates LTP at CA1 hippocampal synapses through activation of dopamine D4 receptors. Proc Natl Acad Sci U S A. 105: 15587-92.

  1. Quick-Filters:
  2. Cited (2)

Items 1 to 20 of 23 total

per page
Page:
  1. 1
  2. 2

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SLC6A3 / DAT (C-term) antibody

Western blot of human caudate lysate showing specific immunolabeling of the ~88k DAT protein. Rabbit IgG Aff - Purified Hu, Ms, non-Human Primates C, Dot, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SLC6A3 / DAT antibody

Western blot of human caudate lysate showing specific immunolabeling of the ~88k DAT protein. Rabbit IgG Aff - Purified Hu, non-Human Primates C, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SLC6A3 / DAT antibody

(1ug/ml) staining of Human Substantia nigra lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SLC6A3 / DAT antibody

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes and rat pheochromocytoma PC12 (lanes 3 and 6) cell lysates: 1-3. Anti-Dopamine Transporter (DAT) (extracellular) antibody, (1:200). 4-6. Anti-Dopamine Transporter (DAT) (extracellular) antibody, preincubated with the control peptide antigen. Rabbit Purified Ms, Rt WB
0.2 ml / €435.00
  OriGene Technologies, Inc.

SLC6A3 / DAT antibody

WB Suggested Anti-SLC6A3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

SLC6A3 / DAT antibody

Western Blot: DAT1 Antibody (mAb16) [TA336930] - Analysis of DAT1 expression in rat striatal membrane tissue lysate. Mouse IgG1 mAb16 Purified Hu, Ms, Rt C, E, ICC/IF, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SLC6A3 / DAT (N-term) antibody

SLC6A3 / DAT Rat IgG2a 4F8 Supernatant Hu, Mky, Ms, Rt C, WB
0.1 ml / €500.00
  Acris Antibodies GmbH

SLC6A3 / DAT (N-term) antibody

Brain, cerebellum: Formalin-Fixed Paraffin Embedded (FFPE) Rabbit IgG Aff - Purified Hu E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

SLC6A3 / DAT antibody

SLC6A3 / DAT Rabbit Serum Ms C, E, WB
0.2 ml / €400.00
  Acris Antibodies GmbH

SLC6A3 / DAT antibody

SLC6A3 / DAT Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

SLC6A3 / DAT antibody

Western blot of human caudate lysate showing specific immunolabeling of the ~88k SLC6A3 protein. Using SLC6A3 polyclonal antibody ( Cat # PAB9595 ). Rabbit Hu, Ms C, WB
0.1 ml / €470.00
  Abnova Taiwan Corp.

SLC6A3 / DAT antibody

Western blot of human caudate lysate showing specific immunolabeling of the ~88k SLC6A3 protein. Using SLC6A3 polyclonal antibody ( Cat # PAB9597 ). Rabbit Hu C, WB
0.1 ml / €470.00
  Abnova Taiwan Corp.

SLC6A3 / DAT (N-term) antibody

SLC6A3 / DAT Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

SLC6A3 / DAT antibody

Dopamine Transporter Antibody [6V-23-23] Mouse IgG1 6V-23-23 Purified Ms, Rt ICC/IF, WB
0.1 ml / €400.00
  Immuquest

SLC6A3 / DAT antibody

SLC6A3 / DAT Rat IgG2a 4F8 Supernatant Hu, Mky, Ms, Rt C, P, WB
0.1 ml / €790.00
  Novus Biologicals Inc.

SLC6A3 / DAT (C-term) antibody

Western Blot: Dopamine Transporter Antibody [NB300-254] - Western blot of human caudate lysate showing specific immunolabeling of the ~88k DAT protein. Rabbit Purified Hst, Hu, Mky, Ms C, P, WB
0.1 ml / €380.00
  Novus Biologicals Inc.

SLC6A3 / DAT antibody

Western Blot: Dopamine Transporter Antibody [NB300-255] - Detection of DAT protein in human caudate sample using NB 300-255. Rabbit Purified Hu, Mky C, P, WB
0.1 ml / €380.00
  Novus Biologicals Inc.

SLC6A3 / DAT (C-term) antibody

SLC6A3 / DAT Rabbit IgG Serum Hu, Ms, Rt WB
25 µl / €360.00
  Novus Biologicals Inc.

SLC6A3 / DAT antibody

SLC6A3 / DAT Rabbit Serum Hu, Ms, Rt E, P, WB
0.2 ml / €400.00
  Novus Biologicals Inc.

SLC6A3 / DAT antibody

Immunohistochemistry-Paraffin: Dopamine Transporter Antibody [NBP1-91846] - Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Items 1 to 20 of 23 total

per page
Page:
  1. 1
  2. 2
  • LinkedIn