SLC6A3 / DAT antibody

Principal name

SLC6A3 / DAT antibody

Alternative names for SLC6A3 / DAT antibody

Sodium-dependent dopamine transporter, DA transporter, DAT1, Solute carrier family 6 member 3

SwissProt ID

P23977 (Rat), P27922 (Bovin), Q01959 (Human), Q61327 (Mouse)

Gene ID

6531 (SLC6A3), 13162 (Slc6a3), 24898

Available reactivities

Hu (Human), Mky (Monkey), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Mouse, Rat, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Frozen Sections (C), Paraffin Sections (P), Dot blot (Dot), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)

Background of SLC6A3 / DAT antibody

The +/Cl transporter family, SLC6, includes DAT a monoamine transporter, important for regulating extracellular levels of dopamine. It does so by uptaking dopamine from the syptic cleft via the co-transport of + and Cl down their electrochemical gradient. The removal of dopamine by DAT remains the most important means to control the extracellular lifetime of the neurotransmitter, and notably the ending of dopaminergic neurotransmission. Transporters for serotonin, norepinephrine, GABA and glycine also belong to this family. These receptors have 12 transmembrane spanning domains and intracellular N- and C-termil domains. DAT is also subjected to post translatiol modifications such phosphorylation, important for its regulation. Also, it possesses a large extracellular domain which undergoes N-glycosylation, important for the proper targeting of the transporter to the plasma membrane. DAT is expressed in dopaminergic cell bodies and termils and can therefore serve as a marker for these neurons. DAT is also expressed in the reti, gastrointestil tract, lung, kidney, pancreas and lymphocytes. DAT plays an important role in movement as well as reward, learning and memory. Its malfunction which leads to dopaminergic dysregulation has been associated with ADHD, schizophrenia, as well as Parkinsonâ??s disease. DAT is the main target for cocaine and amphetamine and methamphetamine psychostimulants, which mainly increase locomotor activities.

General readings

1. Miller, G.W. et al. (1997) Immunochemical analysis of dopamine transporter protein in Parkinson's disease. Ann Neurol. 41:530-539.
2. Kwon, O.B. et al (2008) Neuregulin-1 regulates LTP at CA1 hippocampal synapses through activation of dopamine D4 receptors. Proc Natl Acad Sci U S A. 105: 15587-92.

  1. Quick-Filters:
  2. Cited (2)

20 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SLC6A3 / DAT (C-term) antibody

  Rabbit Anti-Dopamine Transporter, C-Terminus, Human Antibody  
Western blot of human caudate lysate showing specific immunolabeling of the ~88k DAT protein. Rabbit IgG Aff - Purified Hu, Ms, non-Human Primates C, Dot, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SLC6A3 / DAT antibody

  Rabbit Anti-Dopamine Transporter, Extracellular Loop 2, Human Antibody  
Western blot of human caudate lysate showing specific immunolabeling of the ~88k DAT protein. Rabbit IgG Aff - Purified Hu, non-Human Primates C, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SLC6A3 / DAT antibody

  Goat Anti-SLC6A3 / DAT Antibody  
(1ug/ml) staining of Human Substantia nigra lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SLC6A3 / DAT antibody

  Rabbit Polyclonal Anti-Dopamine Transporter (DAT) (extracellular)  
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes and rat pheochromocytoma PC12 (lanes 3 and 6) cell lysates: 1-3. Anti-Dopamine Transporter (DAT) (extracellular) antibody, (1:200). 4-6. Anti-Dopamine Transporter (DAT) (extracellular) antibody, preincubated with the control peptide antigen. Rabbit Purified Ms, Rt WB
0.2 ml / €435.00
  OriGene Technologies, Inc.

SLC6A3 / DAT antibody

  Rabbit Polyclonal Anti-SLC6A3 Antibody  
WB Suggested Anti-SLC6A3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

SLC6A3 / DAT antibody

  Mouse Monoclonal SLC6A3/DAT1 Antibody (mAb16)  
Western Blot: DAT1 Antibody (mAb16) [TA336930] - Analysis of DAT1 expression in rat striatal membrane tissue lysate. Mouse IgG1 mAb16 Purified Hu, Ms, Rt C, E, ICC/IF, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SLC6A3 / DAT antibody

  Rabbit Polyclonal Anti-SLC6A3 Antibody  
The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using (SLC6A3 Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: ×200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

SLC6A3 / DAT (N-term) antibody

SLC6A3 / DAT Rat IgG2a 4F8 Supernatant Hu, Mky, Ms, Rt C, WB
0.1 ml / €500.00
  Acris Antibodies GmbH

SLC6A3 / DAT (N-term) antibody

Brain, cerebellum: Formalin-Fixed Paraffin Embedded (FFPE) Rabbit IgG Aff - Purified Hu E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

SLC6A3 / DAT antibody

SLC6A3 / DAT Rabbit Serum Ms C, E, WB
0.2 ml / €400.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

SLC6A3 - human gene knockout kit via CRISPR

SLC6A3 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

SLC6A3 - human gene knockout kit via CRISPR

SLC6A3 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Slc6a3 - mouse gene knockout kit via CRISPR

Slc6a3 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Slc6a3 - mouse gene knockout kit via CRISPR

Slc6a3 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Slc6a3 - mouse gene knockout kit via CRISPR

Slc6a3 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Slc6a3 - mouse gene knockout kit via CRISPR

Slc6a3 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Slc6a3 (GFP-tagged) - Mouse solute carrier family 6 (neurotransmitter transporter, dopamine), member 3 (Slc6a3), 200 uL, >10^7 TU/mL

Lenti ORF particles, Slc6a3 (GFP-tagged) - Mouse solute carrier family 6 (neurotransmitter transporter, dopamine), member 3 (Slc6a3), 200 uL, >10^7 TU/mL  
200 µl / €1,270.00
  OriGene Technologies, Inc.

Lenti ORF particles, Slc6a3 (Myc-DDK-tagged) - Mouse solute carrier family 6 (neurotransmitter transporter, dopamine), member 3 (Slc6a3), 200 uL, >10^7 TU/mL

Lenti ORF particles, Slc6a3 (Myc-DDK-tagged) - Mouse solute carrier family 6 (neurotransmitter transporter, dopamine), member 3 (Slc6a3), 200 uL, >10^7 TU/mL  
200 µl / €1,140.00
  OriGene Technologies, Inc.

Rat Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Slc6a3 (GFP-tagged ORF) - Rat solute carrier family 6 (neurotransmitter transporter, dopamine), member 3 (Slc6a3), 200 uL, >10^7 TU/mL

Lenti ORF particles, Slc6a3 (GFP-tagged ORF) - Rat solute carrier family 6 (neurotransmitter transporter, dopamine), member 3 (Slc6a3), 200 uL, >10^7 TU/mL  
200 µl / €1,280.00
  OriGene Technologies, Inc.

Lenti ORF particles, Slc6a3 (Myc-DDK-tagged ORF) - Rat solute carrier family 6 (neurotransmitter transporter, dopamine), member 3 (Slc6a3), 200 uL, >10^7 TU/mL

Lenti ORF particles, Slc6a3 (Myc-DDK-tagged ORF) - Rat solute carrier family 6 (neurotransmitter transporter, dopamine), member 3 (Slc6a3), 200 uL, >10^7 TU/mL  
200 µl / €1,144.00
  OriGene Technologies, Inc.

20 Item(s)

per page
  • LinkedIn