SLC8A3 / NCX3 antibody

Principal name

SLC8A3 / NCX3 antibody

Alternative names for SLC8A3 / NCX3 antibody

Sodium/calcium exchanger 3, Na(+)/Ca(2+)-exchange protein 3

SwissProt ID

P57103 (Human), P70549 (Rat)

Gene ID

6547 (SLC8A3), 140448 (Slc8a3)

Available reactivities

Can (Canine), GP (Guinea Pig), Hu (Human), Rb (Rabbit), Rt (Rat), Bov (Bovine), Ms (Mouse)

Available hosts

Mouse, Rabbit

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E)

Background of SLC8A3 / NCX3 antibody

Ca2+ has proven to be a universal sigling molecule in excitable and non-excitable cells. However, being that its intracellular concentration is 1000 time lower than the extracellular milieu, it is important for the cell to keep this ratio for proper function. NCX, a +/Ca2+ exchanger is responsible for most of the efflux of Ca2+ out from the cell1-3. The NCX transporter is a member of the SLC8 family of solute carriers which in turn belong to the CaCA superfamily.NCX-1 is one of three +/Ca2+ exchangers (NCX-1, NCX-2, NCX-3) leading to one Ca2+ movement across the plasma membrane in exchange of three + influx. However, the transporter can reverse the direction of the transport if the concentrations of + and Ca2+ change6. The transporter has nine transmembrane domains and intracellular N- and C-termils. Between tansmembrane domains 5 and 6, the presence of an extra-long intracellular loop, termed the f loop is responsible for regulating the activity of NCX-1 via several different mechanisms like ion binding, phosphorylation, etc. The f loop also has sites which undergo altertive splicing.Of the three NCX-1 expressed in mammalian cells, NCX-1 is the most widely expressed. Its expression is detected in the heart, brain, and kidney. NCX-1 undergoes altertive splicing in a tissue dependent manner. The first splice region does not change the overall structure of the protein but rather ebles the expression of the gene specific to the tissues which require the expression of the gene. The second splicing site leads to a number of proteins varying in length. NCX-2 expression is much more limited; it is expressed only in neurons. NCX-3 is expressed in skeletal muscle and in some regions of the brain and undergoes altertive splicing in a similar fashion to that of NCX-1.Due to their central role in modulating Ca2+ levels in the cell, NCX exchangers are involved in various pathophysiological diseases/disorders such as hypoxia, aging, alzheimerâ??s.to me a few.

General readings

This product has been used in:Frank JS et al. Distribution of the Na(+)-Ca2+ exchange protein in mammalian cardiac myocytes: an immunofluorescence and immunocolloidal gold-labeling study. J Cell Biol 117:337-45 (1992). PubMed PMID: 1373142
General / background references:Porzig H et al. Mapping of the cardiac sodium-calcium exchanger with monoclonal antibodies. Am J Physiol 265:C748-56 (1993). PubMed PMID: 7692739
Li ZP et al. The cardiac Na+-Ca2+ exchanger binds to the cytoskeletal protein ankyrin. J Biol Chem 268:11489-91 (1993). PubMed PMID: 8505285
Li Z et al. Expression of the cardiac Na(+)-Ca2+ exchanger in insect cells using a baculovirus vector. J Biol Chem 267:7828-33 (1992). PubMed PMID: 1560015

10 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SLC8A3 / NCX3 antibody

Predicted cell location: Cytoplasm,Nucleus. Positive control: Human lung cancer tissue. Recommended dilution: 1/100-300  The image on the left is immunohistochemistry of paraffin-embedded human lung cancer tissue using SLC8A3 antibody at dilution 1/70, on the right is treated with the synthetic peptide. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

SLC8A3 / NCX3 antibody

Predicted cell location: Cytoplasm. Positive control: Human liver cancer tissue. Recommended dilution: 1/50-200  The image on the left is immunohistochemistry of paraffin-embedded human liver cancer tissue using SLC8A3 antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

SLC8A3 / NCX3 antibody

Western blot analysis of rat brain lysates (lanes 1 and 4), mouse brain lysates (lanes 2 and 5) and human SH-SY5Y neuroblastoma cell lysates (lanes 3 and 6): 1-3. Anti-Na+/Ca2+ Exchanger 3 (NCX-3) antibody , (1:200). 4-6. Anti-Na+/Ca2+ Exchanger 3 (NCX-3) antibody, preincubated with the control peptide antigen. Rabbit Purified Hu, Ms, Rt WB
0.2 ml / €435.00
  OriGene Technologies, Inc.

SLC8A3 / NCX3 antibody

WB Suggested Anti-SLC8A3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: 721_B cell lysate Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

SLC8A3 / NCX3 antibody

Human 721_B; WB Suggested Anti-SLC8A3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: 721_B cell lysate; SLC8A3 antibody - N-terminal region (ARP44074_P050) in Human 721_B cells using Western Blot Rabbit Aff - Purified Bov, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

SLC8A3 / NCX3 antibody

SLC8A3 / NCX3 Mouse IgM C2C12 Ascites Can, GP, Hu, Rb, Rt ICC/IF, IP, P, WB
0.1 ml / €400.00
  Novus Biologicals Inc.

SLC8A3 / NCX3 antibody

Immunohistochemistry-Paraffin: Sodium-Calcium Exchanger Antibody [NBP1-87808] - Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in enteroendocrine cells. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

SLC8A3 / NCX3

SLC8A3 / NCX3 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

SLC8A3 / NCX3

SLC8A3 / NCX3 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

SLC8A3 overexpression lysate

SLC8A3 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

10 Item(s)

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