SLC9A1 / NHE1 antibody

Principal name

SLC9A1 / NHE1 antibody

Alternative names for SLC9A1 / NHE1 antibody

Sodium/hydrogen exchanger 1, APNH, APNH1, Solute carrier family 9 member 1, Na(+)/H(+) exchanger 1, Na(+)/H(+) antiporter, amiloride-sensitive

SwissProt ID

P19634 (Human), P23791 (Rabit), P26431 (Rat), P48762 (Pig), Q28036 (Bovin), Q61165 (Mouse)

Gene ID

6548 (SLC9A1), 397458, 24782 (Slc9a1)

Available reactivities

Hu (Human), Mam (Mammalia), Por (Porcine), Ms (Mouse), Rt (Rat), Vertebrates, Ve, Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C)

Background of SLC9A1 / NHE1 antibody

In order to function in optimal conditions, cells must maintain a close to neutral intracellular pH. They have adopted various mechanisms in order to do so, one of which is via +/H+ exchangers (NHEs). Genes belonging to this group are expressed along a very broad range of organisms and are essential for protecting cells against intracellular acidification.To date, nine genes have been identified in mammals; NHE1-9. These membrane proteins have 10-12 transmembrane domains depending on whether a splice variant is expressed and an intracellular N-termil. The C-termil domain can be either intracellular or extracellular, also depending whether a splice variant of the protein is involved. The C-termil part of the protein also undergoes posttranslatiol modification such as phosphorylation.Both NHE-1 and NHE-2 have an extracellular loop which is glycosylated.Under physiological conditions, the +/H+ exchanger mediates the exchange of one extracellular + ion for one intracellular proton, thereby keeping the overall charge neutral1. The extracellular binding site of + is not selective as it can also bind Li+ and H+ .K+ ions inhibit NHE-1 but have no effect on NHE-2.The activation of NHE-1 and NHE-2 is sensitive to intracellular acidic pH. Under physiological conditions, both exchangers are not active and upon a drop of intracellular pH, they are rapidly activated.NHE-1 expression is ubiquitous and may serve as a housekeeping gene.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SLC9A1 / NHE1 (N-term) antibody

Western blot analysis of Nhe-1 in rat kidney tissue lysate with in with Nhe-1 antibody at (A) 1 and (B) 2 ug/ml. Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SLC9A1 / NHE1 (Center) antibody

Western blot analysis of Nhe-1 in MOLT4 cell lysate with in with Nhe-1 antibody at (A) 1 and (B) 2 ug/ml. Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SLC9A1 / NHE1 antibody

Western blot analysis of rat brain membranes (lanes 1 and 6), mouse brain lysate (lanes 2 and 7), human MCF-7 breast adenocarcinoma cells (lanes 3 and 8), human U-87 MG glioblastoma cells (lanes 4 and 9) and human THP-1 acute monocytic leukemia cells (lanes 5 and 10): 1-5. Anti-Na+/H+ Exchanger 1 (NHE-1) (extracellular) antibody , (1:200). 6-10. Anti-Na+/H+ Exchanger 1 (NHE-1) (extracellular) antibody, preincubated with the control peptide antigen. Rabbit Purified Hu, Ms, Rt WB
0.2 ml / €435.00
  OriGene Technologies, Inc.

SLC9A1 / NHE1 antibody

WB Suggested Anti-SLC9A1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: PANC1 cell lysate;SLC9A1 is supported by BioGPS gene expression data to be expressed in PANC1 Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

SLC9A1 / NHE1 antibody

SLC9A1 / NHE1 Mouse IgG1 4E9 Purified Mam, Por WB
0.1 mg / €590.00
  Acris Antibodies GmbH

SLC9A1 / NHE1 Control Peptide

SLC9A1 / NHE1 Purified
50 µg / €210.00
  Acris Antibodies GmbH

SLC9A1 / NHE1 antibody

Western blot using NHE1 antibody (AP05294PU-N) on HT-29 cell lysate. Lysate used at 30 µg/ml. Antibody used at 1:400 dilution. Secondary antibody, mouse anti-rabbit HRP, used at 1:50k dilution. Rabbit Aff - Purified Hu WB
10 Blots / €480.00
  Acris Antibodies GmbH

SLC9A1 / NHE1 antibody

Western blot using NHE1 antibody AP05294SU-N on HT-29 cell lysate. Lysate loaded at 30 µg/lane. Antibody used at 10 µg/ml. Secondary antbody: mouse anti-rabbit HRP. Rabbit Purified Hu WB
0.1 mg / €370.00
  Acris Antibodies GmbH

SLC9A1 / NHE1 (C-term) antibody

Human Small Intestine (formalin-fixed, paraffin-embedded) stained with SLC9A1 antibody AP22603PU-N at 5 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit Purified Hu, Ms, Rt E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

SLC9A1 / NHE1 Control Peptide

SLC9A1 / NHE1
50 µg / €160.00
  Acris Antibodies GmbH

SLC9A1 / NHE1 Control Peptide

SLC9A1 / NHE1
50 µg / €160.00
  Acris Antibodies GmbH

SLC9A1 / NHE1 antibody

SLC9A1 / NHE1 Mouse Hu WB
0.5 mg / €1,400.00
  Abnova Taiwan Corp.

SLC9A1 / NHE1 antibody

Immunoperoxidase of monoclonal antibody to SLC9A1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3 ug/ml] Mouse IgG1 2F5 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SLC9A1 / NHE1 antibody

Western blot using SLC9A1 polyclonal antibody ( Cat # PAB10402 ) on HT-29 cell lysate. Lysate loaded at 30 µg/lane. Antibody used at 10 µg/ml. Hu
0.1 mg / €400.00
  Abnova Taiwan Corp.

SLC9A1 / NHE1 antibody

Human PANC1; WB Suggested Anti-SLC9A1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: PANC1 cell lysate. SLC9A1 is supported by BioGPS gene expression data to be expressed in PANC1.; SLC9A1 antibody - middle region (ARP43840_P050) in Human PANC1 cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

SLC9A1 / NHE1 antibody

SLC9A1 / NHE1 Rabbit Serum Hu C, P, WB
0.1 ml / €400.00
  Novus Biologicals Inc.

SLC9A1 / NHE1 antibody

SLC9A1 / NHE1 Rabbit IgG Hu C, WB
0.5 mg / €400.00
  Novus Biologicals Inc.

SLC9A1 / NHE1 antibody

Immunohistochemistry-Paraffin: NHE1 Antibody [NBP1-76847] - Human brain tissue using Nhe-1 antibody at 2.5 ug/mL._x000d_ Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

SLC9A1 / NHE1 antibody

Immunohistochemistry-Paraffin: NHE1 Antibody [NBP1-76848] - human brain tissue using Nhe-1 antibody at 2.5 µg/ml. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

SLC9A1 / NHE1

SLC9A1 / NHE1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

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