SLC9A3R1 / NHERF antibody

Principal name

SLC9A3R1 / NHERF antibody

Alternative names for SLC9A3R1 / NHERF antibody

EBP50, NHERF-1, NPHLOP2, Na(+)/H(+) exchange regulatory cofactor NHE-RF1, Ezrin-radixin-moesin-binding phosphoprotein 50, Regulatory cofactor of Na(+)/H(+) exchanger, Sodium-hydrogen exchanger regulatory factor 1, Solute carrier family 9 isoform A3 regulatory factor 1

SwissProt ID

O14745 (Human), P70441 (Mouse), Q28619 (Rabit), Q3SZK8 (Bovin), Q9JJ19 (Rat)

Gene ID

9368 (SLC9A3R1), 26941 (Slc9a3r1), 59114

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit, Mouse, Chicken

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), ELISA (detection) (E(detection))

Background of SLC9A3R1 / NHERF antibody

EBP 50 [ERM (ezrin-radixin-moesin) binding phosphoprotein of 50 kDa] is a PDZ containing protein that is involved in the linkage of integral membrane proteins to the cytoskeleton. EBP50 contains two tandem PDZ domains followed by a carboxy-terminal sequence that binds to members of the ERM family of membrane-cytoskeleton adaptors. The PDZ domains within EBP50 bind to the carboxy termini of such target proteins as beta2 adrenergic receptor, platelet-derived growth factor receptor (PDGFR), and the cystic fibrosis conductance regulator (CFTR). The PDZ domains are also believed to be involved in the oligomerization of EBP 50 to form multiprotein complexes which helps to facilitate the formation of functional signalling complexes. EBP 50 has been shown to link integral membrane proteins to the cytoskeleton by binding to members of the ERM family of proteins, which bind to F-actin. Through these interactions, EBP 50 is implicated in the localization of interactive groups of proteins into subcellular domains and in the regulation of activity of those interacting proteins. Immunohistochemical studies have shown that EBP 50 is found exclusively at the apical membranes of epithelial cells.

General readings

General / background references:Fouassier L et al. Ezrin-radixin-moesin-binding phosphoprotein 50 is expressed at the apical membrane of rat liver epithelia. Hepatology 33:166-76 (2001). PubMed PMID: 11124833
Fouassier L et al. Evidence for ezrin-radixin-moesin-binding phosphoprotein 50 (EBP50) self-association through PDZ-PDZ interactions. J Biol Chem 275:25039-45 (2000). PubMed PMID: 10859298
Bretscher A et al. ERM-Merlin and EBP50 protein families in plasma membrane organization and function. Annu Rev Cell Dev Biol 16:113-43 (2000). PubMed PMID: 11031232

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SLC9A3R1 / NHERF antibody

Western blot analysis of NHERF1 using rat kidney tissue lysate. Rabbit Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SLC9A3R1 / NHERF antibody

Western blotting of rat kidney membranes: 1. Anti-NHERF-1 antibody (APZ-006), (1:200). 2. Anti NHERF-1 antibody, preincubated with the control peptide antigen. Rabbit Purified Rt WB
0.2 ml / €435.00
  OriGene Technologies, Inc.

SLC9A3R1 / NHERF antibody

Host: Rabbit; Target Name: SLC9A3R1; Sample Tissue: Esophagus Tumor lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

SLC9A3R1 / NHERF antibody

Formalin-Fixed Paraffin-Embedded Human Placenta stained with NHERF antibody Cat.-No AM08325PU at 5 µg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Mouse IgG2b EBP-10 Purified Hu IP, P, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

SLC9A3R1 / NHERF (full length) antibody

Formalin-Fixed Paraffin-Embedded Human Placenta stained with NHERF antibody Cat.-No AM08325PU at 5 µg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Mouse IgG2b EBP-10 Purified Hu IP, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

SLC9A3R1 / NHERF antibody

SLC9A3R1 / NHERF Mouse IgG2b EBP10 Purified Hu IP, P, WB
0.1 mg / €260.00
  Acris Antibodies GmbH

SLC9A3R1 / NHERF antibody

SLC9A3R1 MaxPab polyclonal antibody. Western Blot analysis of SLC9A3R1 expression in human placenta. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

SLC9A3R1 / NHERF antibody

Immunoperoxidase of monoclonal antibody to SLC9A3R1 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml] Mouse IgG2a 2B7 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SLC9A3R1 / NHERF antibody

SLC9A3R1 / NHERF Rabbit Hu, Ms, Rt E, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

SLC9A3R1 / NHERF antibody

Western Blot: Placenta protein extract as test antigen. Affinity-purified IgY dilution: 1:500, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development. ( anti-SLC9A3R1 ) Chicken Aff - Purified Hu E, WB
50 µg / €520.00
  Novus Biologicals Inc.

SLC9A3R1 / NHERF antibody

Western Blot: EBP 50 Antibody [NB300-536] - Figure 2 shows a Western blot of recombinant human EBP50 using NB300-536. Rabbit Aff - Purified Hu, Ms ICC/IF, IP, P, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

SLC9A3R1 / NHERF antibody

SLC9A3R1 / NHERF Mouse IgG2b EBP-10 Aff - Purified Hu IP, P, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

SLC9A3R1 / NHERF antibody

Immunohistochemistry-Paraffin: NHERF-1 Antibody [NBP1-84918] -  staining of human kidney shows distinct cytoplasmic and membranous positivity in tubular cells. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

SLC9A3R1 / NHERF antibody

Immunohistochemistry-Paraffin: NHERF-1 Antibody [NBP1-84919] - Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells of tubules. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

SLC9A3R1 / NHERF antibody

Immunohistochemistry-Paraffin: NHERF-1 Antibody (EPR5562) [NBP1-95758] human kidney tissue using anti-NHERF1. Rabbit EPR5562 Purified Hu, Rt P, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

SLC9A3R1 / NHERF

SLC9A3R1 / NHERF Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

SLC9A3R1 / NHERF

SLC9A3R1 / NHERF Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

SLC9A3R1 / NHERF

SLC9A3R1 / NHERF Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

SLC9A3R1 / NHERF

SLC9A3R1 / NHERF Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

SLC9A3R1 / NHERF

SLC9A3R1 / NHERF Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

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