SLIT1 antibody

Principal name

SLIT1 antibody

Alternative names for SLIT1 antibody

SLIT-1, KIAA0813, MEGF4, SLIL1, Slit homolog 1 protein, Multiple epidermal growth factor-like domains protein 4

SwissProt ID

O75093 (Human), O88279 (Rat), Q80TR4 (Mouse)

Gene ID

20562 (Slit1), 6585 (SLIT1)

Available reactivities

Ms (Mouse), Rt (Rat), Chk (Chicken), Frog, Hu (Human), Ze (Zebrafish), African clawed frog, Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of SLIT1 antibody

SLIT-1 (also known as KIAA0813, MEGF4, multiple epidermal growth factor-like domains 4 and Slit homolog 1 protein) is a Slit protein. This protein is a ligand for the Roundabout (Robo) receptors and acts as guidance cues in axonal migration/navigation during neural development, at the ventral midline of the neural tube. Slit1 and Slit2 are essential for midline guidance in the forebrain by acting as repulsive signals preventing inappropriate midline crossing by axons projecting from the olfactory bulb. Each SLIT gene encodes a putative secreted protein, which contains conserved protein-protein interaction domains including leucine-rich repeats and epidermal growth factor-like motifs, similar to those of the Drosophila protein. In situ hybridization studies indicated that the rat SLIT-1 mRNA was specifically expressed in the neurons of fetal and adult forebrains. This data suggests that the SLIT genes form an evolutionarily conserved group in vertebrates and invertebrates, and that the mammalian SLIT proteins may participate in the formation and maintenance of the nervous and endocrine systems by protein-protein interactions. Alternative splicing isoforms have been identified for Slit1 protein.

General readings

1. Long,H., Sabatier,C., Ma,L., Plump,A., Yuan,W., Ornitz,D.M., Tamada,A., Murakami,F., Goodman,C.S. and Tessier-Lavigne,M. (2004) Conserved roles for Slit and Robo proteins in midline commissural axon guidance. Neuron 42 (2), 213-223.
2. Nguyen-Ba-Charvet,K.T., Picard-Riera,N., Tessier-Lavigne,M., Baron-Van Evercooren,A., Sotelo,C. and Chedotal,A. (2004) Multiple roles for slits in the
control of cell migration in the rostral migratory stream. J. Neurosci. 24 (6), 1497-1506.
3. Knoll,B., Schmidt,H., Andrews,W., Guthrie,S., Pini,A., Sundaresan,V. and Drescher,U. (2003) On the topographic targeting of basal vomeronasal axons through Slit-mediated chemorepulsion. Development 130 (21), 5073-5082.
4. Luukko,K., Loes,S., Furmanek,T., Fjeld,K., Kvinnsland,I.H. and Kettunen,P. (2003) Identification of a novel putative signaling center, the tertiary enamel knot in the postnatal mouse molar tooth. Mech. Dev. 120 (3), 270-276.
5. Nguyen-Ba-Charvet,K.T., Plump,A.S., Tessier-Lavigne,M. and Chedotal,A. (2002) Slit1 and slit2 proteins control the development of the lateral olfactory tract. J. Neurosci. 22 (13), 5473-5480.
6. Causeret,F., Danne,F., Ezan,F., Sotelo,C. and Bloch-Gallego,E. (2002) Slit antagonizes netrin-1 attractive effects during the migration of inferior olivary neurons. Dev. Biol. 246 (2), 429-440.
7. Little,M., Rumballe,B., Georgas,K., Yamada,T. and Teasdale,R.D. (2002) Conserved modularity and potential for alternate splicing in mouse and human Slit genes. Int. J. Dev. Biol. 46 (4), 385-391.
8. Yuan,W., Zhou,L., Chen,J.H., Wu,J.Y., Rao,Y. and Ornitz,D.M. (1999) The mouse SLIT family: secreted ligands for ROBO expressed in patterns that suggest a role in morphogenesis and axon guidance. Dev. Biol. 212 (2), 290-306.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SLIT1 (487-504) antibody

WB using Anti-SLIT-1 antibody shows detection of SLIT-1 in rat (lane 1) and mouse (lane 2) brain lysates.  The expected molecular weight for SLIT-1 is 168 kDa.  Approximately 20 µg of each lysates was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-SLIT-1 antibody. Signal was detected using standard techniques. Note: The smaller strong bands observed in this blot are likely SLIT-1 cleavage products. Rabbit IgG Aff - Purified Ms E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SLIT1 antibody

WB Suggested Anti-SLIT1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Human Stomach Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

SLIT1 (487-504) antibody

SLIT1 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Pancreas. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

SLIT1 antibody

Western blot using Slit1 polyclonal antibody ( Cat # PAB11326 ) shows detection of Slit1 in rat ( lane 1 ) and mouse ( lane 2 ) brain lysates.
The expected molecular weight for Slit1 is 168 kDa.
Approximately 20 µg of each lysates was run on a SDS-PAGE and reaction with a 1 : 500 dilution of Slit1 polyclonal antibody.
Note: The smaller strong bands observed in this blot are likely Slit1 cleavage products. A number of cleavage products for both Slit1 and Slit2 are reported in the literature resulting from alternate splicing and range from ~40 kDa -160 kDa ( see Little et al, 2002 for additional details ). Rabbit Chk, Frog, Hu, Ms, Rt E, WB
0.1 mg / €560.00
  Abnova Taiwan Corp.

SLIT1 antibody

Mouse; Immunohistochemistry with SLIT1 GFP/ WT mice tissue at an antibody concentration of 2.5 ug/ml using anti-SLIT1 antibody (ARP58765_P050); SLIT1 antibody - middle region (ARP58765_P050) in Mouse cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Chk, Hu, Ms, Rt, Ze P, WB
50 µg / €350.00
  AVIVA Systems Biology

SLIT1 antibody

Western Blot: SLIT1 Antibody [NB600-880] - NB 600-880  Slit1 Rabbit polyclonal to Slit1 (1/500) Rabbit IgG Aff - Purified Ms, Rt E, WB
0.1 mg / €510.00
  Novus Biologicals Inc.

SLIT1 antibody

Immunohistochemistry-Paraffin: SLIT1 Antibody [NBP1-81242] - Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells. Rabbit IgG Aff - Purified Hu ICC/IF, P
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

SLIT1 (His-tagged)

Slit1 Enhances Neurite Outgrowth. A: Chick dorsal rootganglion neurons were grown on nitrocellulose-coated plates in the presence of recombinant human NGF-b  B: Neurite outgrowth is enhanced when the plate is also coated with recombinant mouse Slit1 (90 ng/3 μL for 2 hours). Human Purified > 80 % CHO cells
50 µg / €400.00
  Neuromics Antibodies

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