SLIT1 antibody

Principal name

SLIT1 antibody

Alternative names for SLIT1 antibody

SLIT-1, KIAA0813, MEGF4, SLIL1, Slit homolog 1 protein, Multiple epidermal growth factor-like domains protein 4

SwissProt ID

O75093 (Human), O88279 (Rat), Q80TR4 (Mouse)

Gene ID

6585 (SLIT1), 20562 (Slit1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Chk (Chicken), Frog, Ze (Zebrafish), African clawed frog, Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P)

Background of SLIT1 antibody

SLIT-1 (also known as KIAA0813, MEGF4, multiple epidermal growth factor-like domains 4 and Slit homolog 1 protein) is a Slit protein. This protein is a ligand for the Roundabout (Robo) receptors and acts as guidance cues in axonal migration/navigation during neural development, at the ventral midline of the neural tube. Slit1 and Slit2 are essential for midline guidance in the forebrain by acting as repulsive signals preventing inappropriate midline crossing by axons projecting from the olfactory bulb. Each SLIT gene encodes a putative secreted protein, which contains conserved protein-protein interaction domains including leucine-rich repeats and epidermal growth factor-like motifs, similar to those of the Drosophila protein. In situ hybridization studies indicated that the rat SLIT-1 mRNA was specifically expressed in the neurons of fetal and adult forebrains. This data suggests that the SLIT genes form an evolutionarily conserved group in vertebrates and invertebrates, and that the mammalian SLIT proteins may participate in the formation and maintenance of the nervous and endocrine systems by protein-protein interactions. Alternative splicing isoforms have been identified for Slit1 protein.

General readings

1. Long,H., Sabatier,C., Ma,L., Plump,A., Yuan,W., Ornitz,D.M., Tamada,A., Murakami,F., Goodman,C.S. and Tessier-Lavigne,M. (2004) Conserved roles for Slit and Robo proteins in midline commissural axon guidance. Neuron 42 (2), 213-223.
2. Nguyen-Ba-Charvet,K.T., Picard-Riera,N., Tessier-Lavigne,M., Baron-Van Evercooren,A., Sotelo,C. and Chedotal,A. (2004) Multiple roles for slits in the
control of cell migration in the rostral migratory stream. J. Neurosci. 24 (6), 1497-1506.
3. Knoll,B., Schmidt,H., Andrews,W., Guthrie,S., Pini,A., Sundaresan,V. and Drescher,U. (2003) On the topographic targeting of basal vomeronasal axons through Slit-mediated chemorepulsion. Development 130 (21), 5073-5082.
4. Luukko,K., Loes,S., Furmanek,T., Fjeld,K., Kvinnsland,I.H. and Kettunen,P. (2003) Identification of a novel putative signaling center, the tertiary enamel knot in the postnatal mouse molar tooth. Mech. Dev. 120 (3), 270-276.
5. Nguyen-Ba-Charvet,K.T., Plump,A.S., Tessier-Lavigne,M. and Chedotal,A. (2002) Slit1 and slit2 proteins control the development of the lateral olfactory tract. J. Neurosci. 22 (13), 5473-5480.
6. Causeret,F., Danne,F., Ezan,F., Sotelo,C. and Bloch-Gallego,E. (2002) Slit antagonizes netrin-1 attractive effects during the migration of inferior olivary neurons. Dev. Biol. 246 (2), 429-440.
7. Little,M., Rumballe,B., Georgas,K., Yamada,T. and Teasdale,R.D. (2002) Conserved modularity and potential for alternate splicing in mouse and human Slit genes. Int. J. Dev. Biol. 46 (4), 385-391.
8. Yuan,W., Zhou,L., Chen,J.H., Wu,J.Y., Rao,Y. and Ornitz,D.M. (1999) The mouse SLIT family: secreted ligands for ROBO expressed in patterns that suggest a role in morphogenesis and axon guidance. Dev. Biol. 212 (2), 290-306.

12 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SLIT1 (487-504) antibody

  Rabbit polyclonal anti-SLIT-1 antibody  
WB using Anti-SLIT-1 antibody shows detection of SLIT-1 in rat (lane 1) and mouse (lane 2) brain lysates.  The expected molecular weight for SLIT-1 is 168 kDa.  Approximately 20 µg of each lysates was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-SLIT-1 antibody. Signal was detected using standard techniques. Note: The smaller strong bands observed in this blot are likely SLIT-1 cleavage products. Rabbit IgG Aff - Purified Ms E, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

SLIT1 antibody

  Rabbit Polyclonal Anti-SLIT1 Antibody - middle region  
WB Suggested Anti-SLIT1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Human Stomach Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

SLIT1 antibody

  Rabbit Polyclonal Anti-SLIT1 Antibody  
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using (SLIT1 Antibody) at dilution 1/35, on the right is treated with synthetic peptide. (Original magnification: ×200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

SLIT1 (487-504) antibody

SLIT1 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Pancreas. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

SLIT1 - human gene knockout kit via CRISPR

SLIT1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

SLIT1 - human gene knockout kit via CRISPR

SLIT1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

SLIT1 - human gene knockout kit via CRISPR

SLIT1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

SLIT1 - human gene knockout kit via CRISPR

SLIT1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Slit1 - mouse gene knockout kit via CRISPR

Slit1 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

SLIT1 (His-tagged)

Slit1 Enhances Neurite Outgrowth. A: Chick dorsal rootganglion neurons were grown on nitrocellulose-coated plates in the presence of recombinant human NGF-b  B: Neurite outgrowth is enhanced when the plate is also coated with recombinant mouse Slit1 (90 ng/3 μL for 2 hours). Human Purified > 80 % CHO cells
50 µg / €400.00
  Neuromics Antibodies

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, SLIT1 (mGFP-tagged)-Human slit homolog 1 (Drosophila) (SLIT1), 200 uL, >10^7 TU/mL

Lenti ORF particles, SLIT1 (mGFP-tagged)-Human slit homolog 1 (Drosophila) (SLIT1), 200 uL, >10^7 TU/mL  
200 µl / €1,320.00
  OriGene Technologies, Inc.

Lenti ORF particles, SLIT1 (Myc-DDK-tagged)-Human slit homolog 1 (Drosophila) (SLIT1), 200 uL, >10^7 TU/mL

Lenti ORF particles, SLIT1 (Myc-DDK-tagged)-Human slit homolog 1 (Drosophila) (SLIT1), 200 uL, >10^7 TU/mL  
200 µl / €1,180.00
  OriGene Technologies, Inc.

12 Item(s)

per page
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