SMAD1 antibody

Principal name

SMAD1 antibody

Alternative names for SMAD1 antibody

SMAD family member 1, SMAD-1, SMAD 1, MADH1, MAD homolog 1, MADR1, Mad-related protein 1, BSP1, BSP-1, JV4-1, Mothers against decapentaplegic homolog 1, Mothers against DPP homolog 1, Transforming growth factor-beta-signaling protein 1

SwissProt ID

P70340 (Mouse), P97588 (Rat), Q15797 (Human), Q1JQA2 (Bovin), Q9I8V2 (Danre)

Gene ID

4086 (SMAD1), 4090 (SMAD5), 17125 (Smad1), 6154086 (ade), 30628 (smad1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Chk (Chicken), Por (Porcine), Ze (Zebrafish), African clawed frog, Mky (Monkey), Dros (Drosophila), Bov (Bovine), Chimp (Chimpanzee), Eq (Equine), Macaque, Op (Opossum), Orang-Utan, Sh (Sheep)

Available hosts

Rabbit, Mouse, Goat

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Immunoprecipitation (IP), ELISA (detection) (E(detection)), Frozen Sections (C)

Background of SMAD1 antibody

SMAD1 belongs to the SMAD family. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. SMAD1 mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, SMAD1 can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of SMAD1 forms a complex with SMAD4, which is important for its function in the transcription regulation. SMAD1 is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation.The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene \'mothers against decapentaplegic\' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed.The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene \'mothers against decapentaplegic\' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed.

General readings

Jadlowiec,J.A., et al., (2006) J. Biol. Chem. 281 (9), 5341-5347

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SMAD1 antibody

Western blot analysis of extracts of various cell lines, using SMAD1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

SMAD1 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-SMAD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA507244) Mouse IgG2b OTI1C5 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SMAD1 antibody

Immunoperoxidase of monoclonal antibody to SMAD1 on formalin-fixed paraffin-embedded human salivary gland tissue. [antibody concentration 3ug/ml] Mouse IgG1 1D3 Purified Hu E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SMAD1 antibody

Immunoperoxidase of monoclonal antibody to SMAD1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml] Mouse IgG1 2E9 Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SMAD1 antibody

Immunoperoxidase of monoclonal antibody to SMAD1 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 1.5 ug/ml] Mouse IgG2b 2A1 Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SMAD1 antibody

SMAD1 monoclonal antibody (M06), clone 1B8. Western Blot analysis of SMAD1 expression in NIH/3T3 ( Cat # L018V1 ). Mouse IgG1 1B8 Purified Hu, Ms, Rt E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SMAD1 antibody

Western blot analysis of extracts from 293 cells, using Smad1 (Ab-187) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SMAD1 antibody

Western blot analysis of extracts from COS7 cells, treated with EGF (200ng/ml, 15mins), using Smad1 (Ab-465) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SMAD1 antibody

Western blot analysis of extracts from HeLa cells, using Smad1/5/9 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SMAD1 antibody

WB using Anti-SMAD1 antibody shows detection of endogenous SMAD1 in whole cell lysates from human hepatoma (HEPG2, lanes 1-4) and keratinocyte (HaCaT, lanes 5-8) derived cell lines treated with PBS, BMP2, EGF, or NaCl for 1 h at 37? before harvest. Each lane contains approximately 15 µg of lysate. Primary antibody was used at 1:500. Anti-beta actin staining was used as a loading control. The membrane was washed and reacted with a 1:3,000 dilution HRP-conjugated a-Rabbit IgG. Rabbit IgG Aff - Purified Bonobo, Can, Cattle, Chimp, Chk, Dros, Duckbill platypus, Eq, Gorilla, Hu, Macaque, Ms, Op, Orang-Utan, Por, Rt, Salmon, Sh E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SMAD1 pSer206 antibody

WB using Anti-SMAD1 pS206 antibody shows detection of endogenous phosphorylated SMAD1 in whole cell lysates from HEPG2 (lanes 1-4) derived cell lines treated with PBS, BMP2 (5ng/mL), EGF (1ng/mL), or NaCl for 1h at 37? before harvest. Primary antibody was used at 1:500 and pre-incubated before reacting with blot as follows: top row - with PBS,middle row - with the immunizing phosphorylated peptide and bottom row - with control or non-phosphorylated peptide. HRP-conjugated-Rabbit IgG was 1:3,000. Rabbit IgG Aff - Purified Bonobo, Can, Cattle, Chimp, Chk, Dros, Duckbill platypus, Eq, Gorilla, Hu, Macaque, Ms, Op, Orang-Utan, Por, Rt, Salmon, Sh E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SMAD1 antibody

Predicted band size: 52 kDa . Positive control: Hela and 3T3 cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lane1,2: Hela and 3T3 cells lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SMAD1 antibody

Western blot analysis of Smad1 expression in EGF treated COS7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SMAD1 antibody

SMAD1 Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SMAD1 antibody

Western Blot: SMAD1/5/8/9 Antibody [TA336360] - Lysate fromA549 cells probed with SMAD antibody at 2 ug/ml. Rabbit Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SMAD1 antibody

Western Blot: SMAD1/5/8/9 Antibody [TA336440] - analysis using SMAD antibody. Lysate from Saos-2 cells probed with SMAD antibody at 1:1000. Rabbit Purified Hu, Ms, Rt WB
0.2 ml / €325.00
  OriGene Technologies, Inc.

SMAD1 antibody

Western Blot: SMAD1/5/8/9 Antibody [TA336694] - analysis using SMAD antibody. Lysate from A549 cells probed with antibody at 0.5 ug/ml. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test. Rabbit Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SMAD1 antibody

SMAD1 Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SMAD1 antibody

Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-SMAD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA507240) Mouse IgG1 OTI2D2 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SMAD1 antibody

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SMAD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA507243) Mouse IgG1 OTI1C2 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

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