SMAD2 antibody

Principal name

SMAD2 antibody

Alternative names for SMAD2 antibody

SMAD family member 2, SMAD-2, SMAD 2, MADH2, MAD homolog 2, MADR2, Mad-related protein 2, JV18-1, Mothers against decapentaplegic homolog 2, Mothers against DPP homolog 2

SwissProt ID

O70436 (Rat), Q15796 (Human), Q1W668 (Bovin), Q62432 (Mouse), Q9I9P9 (Danre)

Gene ID

4087 (SMAD2)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Por (Porcine), Rt (Rat), Ze (Zebrafish), African clawed frog, Chimp (Chimpanzee), Mky (Monkey), Rb (Rabbit), Sh (Sheep), Dros (Drosophila)

Available hosts

Goat, Mouse, Rabbit

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Flow Cytometry (F), Immunoprecipitation (IP), ChIP assay (ChIP), Frozen Sections (C)

Background of SMAD2 antibody

SMAD2 belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene \'mothers against decapentaplegic\' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. SMAD2 mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. SMAD2 is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, SMAD2 is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin.The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene \'mothers against decapentaplegic\' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants encoding the same protein have been observed.

General readings

Eppert K, Scherer SW, Ozcelik H, Pirone R, Hoodless P, Kim H, Tsui LC, Bapat B, Gallinger S, Andrulis IL, Thomsen GH, Wrana JL, Attisano L. MADR2 maps to 18q21 and encodes a TGFbeta-regulated MAD-related protein that is functionally mutated in colorectal carcinoma.
Cell 1996 Aug 23;86(4):543-52
PMID: 8752209

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SMAD2 antibody

Anti-SMAD2 mouse monoclonal antibody (TA501777) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SMAD2(RC204604). Mouse IgG2b OTI4G7 Purified Hu F, ICC/IF, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

SMAD2 antibody

Immunohistochemical analysis of paraffin-embedded human cerebellum tissues using SMAD2 mouse mAb with DAB staining. Mouse IgG1 5G7 Ascites Hu E, ICC/IF, P, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

SMAD2 antibody

Immunoperoxidase of monoclonal antibody to SMAD2 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml] Mouse IgG 4D10 Purified Hu E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SMAD2 antibody

Immunoperoxidase of monoclonal antibody to SMAD2 on formalin-fixed paraffin-embedded human testis. [antibody concentration 5 ug/ml] Mouse IgG2b 3G9 Purified Hu E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SMAD2 antibody

TA302690 staining (2µg/ml) of HeLa lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Hu E, WB
20 µg / €200.00
  Acris Antibodies GmbH

SMAD2 antibody

TA302690 staining (2µg/ml) of HeLa lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SMAD2 antibody

Western blot analysis of extracts from RAW264.7 cells and 293 cells, using Smad2 (Ab-220) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SMAD2 antibody

Western blot analysis of extracts from 293 cells, JurKat cells, HepG2 cells and K562 cells, using Smad2 (Ab-465) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SMAD2 antibody

WB using Anti-Smad2 to detect over-expressed Smad2 in COS cells (arrow). Lane C shows mock infection of COS cells with lentiviral vector alone. Lane S2 shows detection of Smad2 in lysates of COS transfected with Smad2. Lane V contains lysates of MDA-MB231 cells treated with vehicle; the next lane contains lysates of MDA-MB231 cells treated with TGF. Preincubation of the antibody with immunizing peptide (data not shown) completely blocks specific band staining. The primary antibody at 1:2500. Rabbit IgG Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SMAD2 antibody

Predicted band size: 60 kDa . Positive control: 293 and NIH/3T3 cell lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lane1: 293 cell lysate Lane2: NIH/3T3 cell lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SMAD2 antibody

Western blot analysis of SMAD2 Antibody (Cat.# TA324932) in NIH-3T3 cell line lysates (35ug/lane). SMAD2 (arrow) was detected using the purified Pab. Rabbit IgG Purified Bov, Chk, Dros, Hu, Ms, Por, Rt, Ze F, ICC/IF, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

SMAD2 antibody

Western blot analysis of SMAD2 Antibody (T220) (Cat.# TA324933) in NCI-H460 cell line lysates (35ug/lane). SMAD2 (arrow) was detected using the purified Pab. Rabbit IgG Purified Bov, Hu, Ms, Rt, Ze ICC/IF, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

SMAD2 antibody

Western blot analysis of Smad2 expression in HepG2 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SMAD2 antibody

Western blot analysis of Smad2 expression in 293 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SMAD2 antibody

Western Blot: SMAD2 Antibody [TA336363] - analysis using SMAD2 antibody. Lysate from rat muscle in the 1) absence and 2) presence of immunizing peptide probed with SMAD2 antibody at 3 ug/ml. Rabbit Purified Bov, Can, Chk, Hu, Ms, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SMAD2 antibody

Western blot detection of Smad2 in Hela,A431,Jurkat and K562 cell lysates using Smad2 mouse mAb (1:500 diluted).Predicted band size:60KDa.Observed band size:60KDa. Mouse IgG1 6H5-E3-C11 Purified ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SMAD2 antibody

Anti-SMAD2 mouse monoclonal antibody (TA501728) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SMAD2(RC204604). Mouse IgG2b OTI2C10 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SMAD2 (181-280) antibody

Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2b 3G6 Purified Hu E, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

SMAD2 antibody

Western blot (WB) analysis of Smad2 antibody (Cat.-No.: AP01355PU-N) in extracts from COLO cells treated with serum 20% 15' Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €350.00
  Acris Antibodies GmbH

SMAD2 antibody

Immunohistochemistry (IHC) analyzes of Smad2 antibody (Cat.-No.: AP06413PU-N) in paraffin-embedded human prostate carcinoma lung carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

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