SMAD3 antibody

Principal name

SMAD3 antibody

Alternative names for SMAD3 antibody

SMAD family member 3, SMAD 3, SMAD-3, Mad3, MAD-3, MADH3, MAD homolog 3, JV15-2, Mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3

SwissProt ID

P84022 (Human), P84023 (Chick), P84024 (Pig), P84025 (Rat), Q8BUN5 (Mouse), Q9GKR4 (Human)

Gene ID

4088 (SMAD3)

Available reactivities

Hu (Human), Rt (Rat), Ms (Mouse), Can (Canine), Chk (Chicken), Por (Porcine), Ze (Zebrafish), African clawed frog, Bov (Bovine), Frog, Mky (Monkey), Macaque, Xen (Xenopus), Sh (Sheep)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), ChIP assay (ChIP), Immunoprecipitation (IP), ELISA (detection) (E(detection)), Frozen Sections (C)

Background of SMAD3 antibody

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

General readings

1. Finer G  et al. Divergent roles of Smad3 and PI3-kinase in murine adriamycin nephropathy indicate distinct mechanisms of proteinuria and fibrogenesis. Kidney Int : (2012).PubMed: 22534961
2. Nevo Y  et al. Fibrosis inhibition and muscle histopathology improvement in laminin-alpha2-deficient mice. Muscle Nerve 42:218-29 (2010). IHC-P ; Mouse .PubMed: 20589893
3. Lee JM  et al. Lung tissue regeneration after induced injury in Runx3 KO mice. Cell Tissue Res 341:465-70 (2010). IHC (PFA fixed) ; Mouse .PubMed: 20623301
4. Rodríguez-Vita J  et al. Essential role of TGF-beta/Smad pathway on statin dependent vascular smooth muscle cell regulation. PLoS ONE 3:e3959 (2008). IHC-P ; Mouse .PubMed: 19088845

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SMAD3 (417-425) pSer423/425 antibody

Formalin-Fixed Paraffin-Embedded Human Skin stained with SMAD3 antibody Cat.-No AP07817PU-N at 2.5 µg/ml after heat-induced antigen retrieval. Antibody LS-B64 concentration 5 ug/ml. Rabbit Aff - Purified Bov, Chk, Frog, Hu, Ms, Por, Rt, Ze E, F, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

SMAD3 antibody

Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2a 2C12 Purified Hu E, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

SMAD3 antibody

Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG1 7F3 Purified Hu, Rt E, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

SMAD3 antibody

Immunoperoxidase of monoclonal antibody to SMAD3 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 6 ug/ml] Mouse IgG2a 2C12 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SMAD3 antibody

Immunoperoxidase of monoclonal antibody to SMAD3 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml] Mouse IgG1 7F3 Purified Hu, Rt E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SMAD3 antibody

Western Blot analysis of SMAD3 expression in transfected 293T cell line by SMAD3 monoclonal antibody (M07), clone 1B7.

Lane 1: SMAD3 transfected lysate(48 KDa).
Lane 2: Non-transfected lysate. Mouse IgG2b 1B7 Purified Hu, Ms, Rt E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SMAD3 antibody

Immunofluorescence of monoclonal antibody to SMAD3 on HeLa cell. [antibody concentration 10 ug/ml] Mouse IgG2a 1C11 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SMAD3 pSer213 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with SMAD3 (phospho S213) polyclonal antibody ( Cat # PAB0610 ) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma. Rabbit Hu E, P, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

SMAD3 antibody

Human breast ca stained with Anti-Smad3, cat# AP15778PU Rabbit Purified Hu P
0.5 ml / €360.00
  Acris Antibodies GmbH

SMAD3 antibody

Human breast ca stained with Anti-Smad3, cat# AP15778PU Rabbit Purified Hu P
1 ml / €520.00
  Acris Antibodies GmbH

SMAD3 antibody

Human breast ca stained with Anti-Smad3, cat# AP15778PU Rabbit Purified Hu P
0.1 ml / €190.00
  Acris Antibodies GmbH

SMAD3 antibody

Western blot analysis of extracts from HUVEC cells, using Smad3 (Ab-204) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SMAD3 (C-term) antibody

Western blot using affinity purified anti-Smad3 antibody shows detection of endogenous Smad3 in both unstimulated and stimulated cell lysates. Lysates were prepared from control cells (- lanes), or cells stimulated with 2 ng/ml TGF-ß lanes for 1 hour. This reagent recognizes both non-phosphorylated and phosphorylated Smad3 protein. Personal Communication. Ying Zhang, NIH, CCR, Bethesda, MD. Rabbit IgG Aff - Purified Bov, Chk, Hu, Ms, Por, Rt, Xen, Ze E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SMAD3 antibody

Western blot using affinity purified anti-Smad3 to detect over-expressed Smad3 in 231 cells (lane 2). Lane 1 shows mock infection of 231 cells with lentiviral vector alone. The membrane was probed with the primary antibody at a 1:5,000 dilution. Personal Communication, Allan Weissman, CCR-NCI, Bethesda, MD. Personal Communication Rabbit IgG Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SMAD3 antibody

NMuMG mouse mammary epithelial cells were probed for the activation of Smad3 by detecting phosphorylation of threonine 179. The cells were either untreated or treated with TGF-beta, transferred to membranes and probed with Anti-SMAD3 pT179 (RABBIT) Antibody. The antibody detects only Smad3 in stimulated cells suggesting detection of phosphorylated SMAD3 at T179. Rabbit IgG Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SMAD3 antibody

WB of Rabbit Anti-SMAD3 antibody. Lane 1: AML12 unstimulated. Lane 2: AML12 stimulated with TGFB. Lane 3: MEFwt unstimulated. Lane 4: MEFwt stimulated with TGFB. Lane 5: MEF Smad3 KO unstimulated. Lane 6: MEF Smad3 KO stimulated with TGFB. Lane 7: HEK293 Smad3T179A mutant unstimulated. Lane 8: HEK293 Smad3T179A mutant stimulated with TGFB. Lane 9: HEK293 Smad3T179V mutant unstimulated. Lane 10: HEK293 Smad3T179V mutant stimulated with TGFB.  Primary antibody: 1:1000. Secondary antibody:1:10,000. Rabbit IgG Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SMAD3 antibody

Western blot using affinity purified anti-Smad3 pS423 pS425 antibody shows detection of endogenous Smad3 in stimulated cell lysates. Lysates were prepared from control cells (- lanes), or cells stimulated with 2 ng/ml TGF (+lanes) for 1 hour. This reagent recognizes phosphorylated Smad3 and has negligible reactivity against non-phosphorylated Smad3 protein. Personal Communication. Ying Zhang, NIH, CCR, Bethesda, MD. Rabbit IgG Purified Bov, Chk, Hu, Ms, Rt, Xen, Ze E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SMAD3 antibody

Predicted cell location: Cytoplasm; Nucleus. Positive control: Human breast carcinoma tissue. Recommended dilution: 1/ 50-100  The image on the left is immunohistochemistry of paraffin-embedded human breast carcinoma tissue using SMAD3 (Ab-425) antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt ICC/IF, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

SMAD3 antibody

SMAD3 Antibody (S208) (Cat. #TA325124) western blot analysis in HepG2 and mouse C2C12 cell line lysates (35ug/lane).This demonstrates the SMAD3 antibody detected the SMAD3 protein (arrow). Rabbit Ig Aff - Purified Hu F, ICC/IF, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

SMAD3 antibody

Western blot analysis of Smad3 expression in 293 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

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