SMAD3 antibody

Principal name

SMAD3 antibody

Alternative names for SMAD3 antibody

SMAD family member 3, SMAD 3, SMAD-3, Mad3, MAD-3, MADH3, MAD homolog 3, JV15-2, Mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3

SwissProt ID

P84022 (Human), P84023 (Chick), P84024 (Pig), P84025 (Rat), Q8BUN5 (Mouse), Q9GKR4 (Human)

Gene ID

4088 (SMAD3), 17127 (Smad3), 25631

Available reactivities

Hu (Human), Rt (Rat), Ms (Mouse), Can (Canine), Chk (Chicken), Por (Porcine), Ze (Zebrafish), African clawed frog, Bov (Bovine), Frog, Mky (Monkey), Macaque, Sh (Sheep), Xen (Xenopus)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Immunoprecipitation (IP), ELISA (detection) (E(detection))

Background of SMAD3 antibody

Smad3 (also known as Mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, Mad3, hMAD-3, JV15-2 or hSMAD3) is a transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type I receptor kinase. These activators exert diverse effects on a wide array of cellular processes. The Smad proteins mediate much of the signaling responses induced by the TGF-beta superfamily. Briefly, activin type I receptor kinase phosphorylates receptor-activated Smads (R-Smads) at the two extreme serines in the C-terminal SSXS motif; e.g. Smad2 and Smad3 proteins in the TGF-beta pathway, or Smad1, Smad5 or Smad8 in the Bone Morphogenetic Proteins (BMP) pathway. The phosphorylated R-Smad then translocates into the nucleus, where it regulates transcription of target genes. Based on microarray and animal model experiments, Smad3 accounts for at least 80% of all TGF-beta-mediated responses.

General readings

1. Finer G  et al. Divergent roles of Smad3 and PI3-kinase in murine adriamycin nephropathy indicate distinct mechanisms of proteinuria and fibrogenesis. Kidney Int : (2012).PubMed: 22534961
2. Nevo Y  et al. Fibrosis inhibition and muscle histopathology improvement in laminin-alpha2-deficient mice. Muscle Nerve 42:218-29 (2010). IHC-P ; Mouse .PubMed: 20589893
3. Lee JM  et al. Lung tissue regeneration after induced injury in Runx3 KO mice. Cell Tissue Res 341:465-70 (2010). IHC (PFA fixed) ; Mouse .PubMed: 20623301
4. Rodríguez-Vita J  et al. Essential role of TGF-beta/Smad pathway on statin dependent vascular smooth muscle cell regulation. PLoS ONE 3:e3959 (2008). IHC-P ; Mouse .PubMed: 19088845

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SMAD3 (417-425) pSer423/425 antibody

Formalin-Fixed Paraffin-Embedded Human Skin stained with SMAD3 antibody Cat.-No AP07817PU-N at 2.5 µg/ml after heat-induced antigen retrieval. Antibody LS-B64 concentration 5 ug/ml. Rabbit Aff - Purified Bov, Chk, Frog, Hu, Ms, Por, Rt, Ze E, F, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

SMAD3 antibody

Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2a 2C12 Purified Hu E, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

SMAD3 antibody

Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG1 7F3 Purified Hu, Rt E, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

SMAD3 antibody

  Rabbit anti-SMAD3 (Phospho-Ser425) polyclonal antibody(Phospho-specific)  
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Smad3 (phospho-Ser425) antibody. Rabbit IgG Purified Hu, Ms, Rt P
0.1 mg / €325.00
  OriGene Technologies, Inc.

SMAD3 antibody

  Rabbit polyclonal Smad3 (Ab-204) antibody  
Western blot analysis of extracts from HUVEC cells, using Smad3 (Ab-204) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SMAD3 (C-term) antibody

  Rabbit polyclonal anti-SMAD3 antibody  
Western blot using affinity purified anti-Smad3 antibody shows detection of endogenous Smad3 in both unstimulated and stimulated cell lysates. Lysates were prepared from control cells (- lanes), or cells stimulated with 2 ng/ml TGF-ß lanes for 1 hour. This reagent recognizes both non-phosphorylated and phosphorylated Smad3 protein. Personal Communication. Ying Zhang, NIH, CCR, Bethesda, MD. Rabbit IgG Aff - Purified Bov, Chk, Hu, Ms, Por, Rt, Xen, Ze E, P, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

SMAD3 antibody

  Rabbit polyclonal anti-SMAD3 antibody  
Western blot using affinity purified anti-Smad3 to detect over-expressed Smad3 in 231 cells (lane 2). Lane 1 shows mock infection of 231 cells with lentiviral vector alone. The membrane was probed with the primary antibody at a 1:5,000 dilution. Personal Communication, Allan Weissman, CCR-NCI, Bethesda, MD. Personal Communication Rabbit IgG Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

SMAD3 antibody

  Rabbit polyclonal SMAD3 phospho T179 antibody  
NMuMG mouse mammary epithelial cells were probed for the activation of Smad3 by detecting phosphorylation of threonine 179. The cells were either untreated or treated with TGF-beta, transferred to membranes and probed with Anti-SMAD3 pT179 (RABBIT) Antibody. The antibody detects only Smad3 in stimulated cells suggesting detection of phosphorylated SMAD3 at T179. Rabbit IgG Purified Hu E, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

SMAD3 antibody

  Rabbit polyclonal anti-SMAD3 antibody  
WB of Rabbit Anti-SMAD3 antibody. Lane 1: AML12 unstimulated. Lane 2: AML12 stimulated with TGFB. Lane 3: MEFwt unstimulated. Lane 4: MEFwt stimulated with TGFB. Lane 5: MEF Smad3 KO unstimulated. Lane 6: MEF Smad3 KO stimulated with TGFB. Lane 7: HEK293 Smad3T179A mutant unstimulated. Lane 8: HEK293 Smad3T179A mutant stimulated with TGFB. Lane 9: HEK293 Smad3T179V mutant unstimulated. Lane 10: HEK293 Smad3T179V mutant stimulated with TGFB.  Primary antibody: 1:1000. Secondary antibody:1:10,000. Rabbit IgG Purified Hu E, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

SMAD3 antibody

  Rabbit polyclonal SMAD3 phospho S423/phospho S425 antibody  
Western blot using affinity purified anti-Smad3 pS423 pS425 antibody shows detection of endogenous Smad3 in stimulated cell lysates. Lysates were prepared from control cells (- lanes), or cells stimulated with 2 ng/ml TGF (+lanes) for 1 hour. This reagent recognizes phosphorylated Smad3 and has negligible reactivity against non-phosphorylated Smad3 protein. Personal Communication. Ying Zhang, NIH, CCR, Bethesda, MD. Rabbit IgG Purified Bov, Chk, Hu, Ms, Rt, Xen, Ze E, P, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

SMAD3 antibody

  Anti-SMAD3 (Phospho-Ser425) Rabbit Polyclonal Antibody  
Predicted band size: 48 kDa . Positive control: Hela cells treated with EGF lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lane1: Hela cells untreated with EGF lysate Lane2: Hela cells treated with EGF lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SMAD3 antibody

  Anti-SMAD3 Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm; Nucleus. Positive control: Human breast carcinoma tissue. Recommended dilution: 1/ 50-100  The image on the left is immunohistochemistry of paraffin-embedded human breast carcinoma tissue using SMAD3 (Ab-425) antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt ICC/IF, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

SMAD3 antibody

  Rabbit polyclonal SMAD3-S208 Antibody  
SMAD3 Antibody (S208) (Cat. #TA325124) western blot analysis in HepG2 and mouse C2C12 cell line lysates (35ug/lane).This demonstrates the SMAD3 antibody detected the SMAD3 protein (arrow). Rabbit Ig Aff - Purified Hu F, ICC/IF, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

SMAD3 antibody

  Rabbit Polyclonal Smad2/3 (Thr8) Antibody (Phospho-specific)  
Western blot analysis of Smad2/3 phosphorylation expression in RAW264.7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SMAD3 antibody

  Rabbit Polyclonal Smad3 (Ser204) Antibody (Phospho-specific)  
Western blot analysis of Smad3 phosphorylation expression in Serum treated NIH-3T3 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SMAD3 antibody

  Rabbit Polyclonal Smad3 (Ser208) Antibody (Phospho-specific)  
Western blot analysis of Smad3 phosphorylation expression in Jurkat whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SMAD3 antibody

  Rabbit Polyclonal Smad3 Antibody  
Western blot analysis of Smad3 expression in 293 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SMAD3 antibody

  Rabbit Polyclonal Smad2/3 Antibody  
Western blot analysis of Smad2/3 expression in UV treated COS7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SMAD3 antibody

  Rabbit Polyclonal anti-SMAD3 Antibody  
Human Breast Carcinoma stained with anti-SMAD3 antibody. Rabbit Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

SMAD3 antibody

Immunofluorescence analysis of NIH/3T3 cells using SMAD3 antibody Cat.-No AM06479SU-N (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Mouse IgG1 5G11 Ascites Hu, Ms E, ICC/IF, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

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