SMAD4 antibody

Principal name

SMAD4 antibody

Alternative names for SMAD4 antibody

SMAD family member 4, SMAD-4, SMAD 4, MADH4, MAD homolog 4, DPC4, Mothers against decapentaplegic homolog 4, Mothers against DPP homolog 4, Deletion target in pancreatic carcinoma 4

SwissProt ID

O70437 (Rat), P97471 (Mouse), Q13485 (Human), Q1HE26 (Bovin), Q9GKQ9 (Pig)

Gene ID

4089 (SMAD4), 17128

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Por (Porcine), Can (Canine), Frog, Xen (Xenopus), Mky (Monkey), Ze (Zebrafish), Eq (Equine), GP (Guinea Pig), Gt (Goat), Rb (Rabbit), Sh (Sheep)

Available hosts

Rabbit, Mouse, Goat

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P), Dot blot (Dot), Flow Cytometry (F), Frozen Sections (C)

Background of SMAD4 antibody

SMAD4 (also known as Mothers against decapentaplegic homolog 4, Mothers against DPP homolog 4, deletion target in pancreatic carcinoma 4 and hSMAD4) is a common mediator of signal transduction by TGF-beta (transforming growth factor), but is also involved in cancer development and metastases as a tumor suppressor. SMAD4 promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. SMAD4 may form trimers with receptor-regulated SMAD (R-SMAD) and interacts with ATF2, COPS5, DACH1, MSG1, SKI and TRIM33. In the absence of ligand SMAD4 is found in the cytoplasm, but when complexed with R-SMAD, translocates to the nucleus. Defects in SMAD4 are a cause of pancreatic carcinoma and juvenile polyposis syndrome (JPS), a syndrome in which patients are at risk for developing gastrointestinal cancers.

General readings

1. Kim, B.-G.; Li, C.; Qiao, W.; Mamura, M.; Kasprzak, B.; Anver, M.; Wolfraim, L.; Hong, S.; Mushinski, E.; Potter, M.; Kim, S.-J.; Fu, X.-Y.; Deng, C.; Letterio, J. J. : Smad4 signalling in T cells is required for suppression of gastrointestinal cancer. Nature 441: 1015-1019, 2006. Note: Erratum: Nature 444: 780 only, 2006.
2. Houlston, R.; Bevan, S.; Williams, A.; Young, J.; Dunlop, M.; Rozen, P.; Eng, C.; Markie, D.; Woodford-Richens, K.; Rodriguez-Bigas, M. A.; Leggett, B.; Neale, K.; Phillips, R.; Sheridan, E.; Hodgson, S.; Iwama, T.; Eccles, D.; Bodmer, W.; Tomlinson, I. : Mutations in DPC4 (SMAD4) cause juvenile polyposis syndrome, but only account for a minority of cases. Hum. Molec. Genet. 7: 1907-1912, 1998.
3 Howe, J. R.; Roth, S.; Ringold, J. C.; Summers, R. W.; Jarvinen, H. J.; Sistonen, P.; Tomlinson, I. P. M.; Houlston, R. S.; Bevan, S.; Mitros, F. A.; Stone, E. M.; Aaltonen, L. A. : Mutations in the SMAD4/DPC4 gene in juvenile polyposis. Science 280: 1086-1088, 1998.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SMAD4 antibody

Immunohistochemistry (IHC) analyzes of Smad4 antibody (Cat.-No.: AP06725PU-N) in paraffin-embedded human brast carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

SMAD4 antibody

Immunohistochemical analysis of paraffin-embedded lung cancer tissues using SMAD4 antibody Cat.-No AM06501SU-N with DAB staining. Mouse IgG1 4G1C6 Ascites Hu E, ICC/IF, P, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

SMAD4 antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between CDKN1A and SMAD4 HeLa cells were stained with anti-CDKN1A rabbit purified polyclonal 1:1200 and anti-SMAD4 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Mouse IgG2a 3E2 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SMAD4 antibody

Immunohistochemistry-Paraffin: SMAD4 Antibody (4G1C6) [NBP1-51646] - Immunohistochemical analysis of paraffin-embedded lung cancer tissues using SMAD4 mouse mAb with DAB staining. Mouse IgG1 4G1C6 Purified Hu E, F, ICC/IF, P, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

SMAD4 (C-term) antibody

TA302724 staining (0.5µg/ml) of NIH-3T3 cell lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt E, WB
20 µg / €200.00
  Acris Antibodies GmbH

SMAD4 antibody

Western blot analysis of SMAD4 Antibody (T277)(arrow) using rabbit polyclonal SMAD4 Antibody (T277) (Cat.#TA302055). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SMAD4 gene (Lane 2) (Origene Technologies). Rabbit Ig Aff - Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SMAD4 (C-term) antibody

TA302724 staining (0.5µg/ml) of NIH-3T3 cell lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SMAD4 antibody

Predicted cell location: Cytoplasm. Positive control: Human ovarian cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using SMAD4 antibody at dilution 1/30, on the right is treated with the fusion protein. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

SMAD4 (C-term) antibody

Western blot analysis of SMAD4 (arrow) using rabbit polyclonal SMAD4 Antibody (C-term) (Cat. #TA324984). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SMAD4 gene (Lane 2) . Rabbit Hu WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

SMAD4 antibody

Western blot analysis of extracts from HeLa cells, COS-7 cells, HepG2 cells and HUVEC cells, using Smad4 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SMAD4 antibody

WB Suggested Anti-SMAD4 Antibody Titration: 1.25 ug/ml; ELISA Titer: 1:312500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SMAD4 antibody

WB Suggested Anti-Smad4 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Mouse Brain Rabbit IgG Purified Bov, Can, Eq, GP, Gt, Hu, Ms, Rb, Rt, Sh, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

SMAD4 antibody

Western blot analysis of extracts of various cell lines, using SMAD4 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

SMAD4 antibody

WB Suggested Anti-SMAD4 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: 293T cell lysate; SMAD4 is supported by BioGPS gene expression data to be expressed in HEK293T Rabbit IgG Purified Bov, Can, Eq, GP, Gt, Hu, Ms, Rb, Rt, Sh, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

SMAD4 antibody

Host: Rabbit; Target Name: Smad4; Sample Tissue: Mouse Testis lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Can, Eq, GP, Gt, Hu, Ms, Rb, Rt, Sh, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

SMAD4 antibody

Western blot analysis on HeLa cell lysate using Smad4 Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SMAD4 antibody

SMAD4 Mouse IgG1 IMD-89 Purified Hu ICC/IF, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

SMAD4 antibody

SMAD4 Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €370.00
  Acris Antibodies GmbH

SMAD4 antibody

Western blot using affinity purified anti-SMAD4 to detect over-expressed SMAD4 in transfected COS cells (lane 2). Lane 1 contains lysate from mock transfected COS cells. A doublet band is seen in the SMAD4 transfected lysate with the upper band (arrowhead) representing SMAD4 and the lower band being non-specific staining. The membrane was probed with tprimary antibody at a 1:1,000 dilution. Rabbit IgG Aff - Purified Hu, Ms, Xen E, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

SMAD4 pThr277 antibody

Formalin-fixed and paraffin-embedded Breast Carcinoma reacted with SMAD4 pThr277 (center) (Cat.#AP12711PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu P

Regular Price: 0.4 ml / €370.00

Special Price: 0.4 ml / €231.00

  Acris Antibodies GmbH

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