SMAD5 antibody

Principal name

SMAD5 antibody

Alternative names for SMAD5 antibody

SMAD family member 5, SMAD 5, SMAD-5, MADH5, MAD homolog 5, JV5-1, Mothers against decapentaplegic homolog 5, Mothers against DPP homolog 5

SwissProt ID

P97454 (Mouse), Q56I99 (Chick), Q99717 (Human), Q9R1V3 (Rat), Q9W7E7 (Danre)

Gene ID

4090 (SMAD5)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Bov (Bovine), Chk (Chicken), Ze (Zebrafish), African clawed frog, Sh (Sheep)

Available hosts

Rabbit, Mouse, Goat

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Frozen Sections (C), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Paraffin Sections (P)

Background of SMAD5 antibody

Bone morphogenetic proteins (BMPs) constitute a large family of sigling molecules that regulate a wide range of critical processes including morphogenesis, cell-fate determition, proliferation, differentiation, and apoptosis (1,2). BMP receptors are members of the TGF-β family of Ser/Thr kise receptors. Ligand binding induces multimerization, autophosphorylation, and activation of these receptors (3-5). They subsequently phosphorylate Smad1 at Ser463 and Ser465 in the carboxy-termil motif SSXS, as well as Smad5 and Smad8 at their corresponding sites. These phosphorylated Smads dimerize with the coactivating Smad4 and translocate to the nucleus, where they stimulate transcription of target genes (5).MAP kises and CDKs 8 and 9 phosphorylate residues in the linker region of Smad1, including Ser206. The phosphorylation of Ser206 recruits Smurf1 to the linker region and leads to the degradation of Smad1 (6). Phosphorylation of this site also promotes Smad1 transcriptiol action by recruiting YAP to the linker region (7).

General readings

Langenfeld,E.M., et al., (2006) Oncogene 25 (5), 685-692

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SMAD5 antibody

Immunohistochemical analysis of paraffin-embedded brain tissues (left) and lung cancer tissues (right) using SMAD5 antibody Cat.-No AM06562SU-N with DAB staining. Mouse IgG1 3H9 Ascites Hu, Rt E, F, ICC/IF, P, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

SMAD5 antibody

Immunofluorescence of monoclonal antibody to SMAD5 on HeLa cell. [antibody concentration 10 ug/ml] Mouse IgG2a 2D7 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SMAD5 antibody

Predicted band size: 52 kDa . Positive control: Hela cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/250 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 20 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SMAD5 antibody

Predicted cell location: Cytoplasm. Positive control: Human breast and lung cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human breast cancer tissue using SMAD5 antibody at dilution 1/20, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

SMAD5 antibody

WB Suggested Anti-SMAD5 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

SMAD5 antibody

WB Suggested Anti-SMAD5 Antibody; Titration: 2.5 ug/ml; Positive Control: HepG2 Whole Cell Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SMAD5 antibody

Western blot analysis of extracts of K562 cell lines, using SMAD5 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

SMAD5 antibody

Western blot detection of SMAD5 (C-terminus) in Hela,Jurkat and K562 cell lysates using SMAD5 (C-terminus) mouse mAb (1:1000 diluted).Predicted band size:52KDa.Observed band size:60KDa. Mouse IgG1 4B10-B10-B6 Purified F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SMAD5 antibody

SMAD5 Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €390.00
  Acris Antibodies GmbH

SMAD5 antibody

Western Blot analysis of SMAD5 expression in transfected 293T cell line (H00004090-T01) by SMAD5 MaxPab polyclonal antibody.

Lane 1: SMAD5 transfected lysate(51.15 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

SMAD5 antibody

Immunofluorescence of monoclonal antibody to SMAD5 on HeLa cell. [antibody concentration 10 ug/ml] Mouse IgG2a 3A10 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SMAD5 antibody

SMAD5 monoclonal antibody (M05), clone 4F10 Western Blot analysis of SMAD5 expression in HeLa ( Cat # L013V1 ). Mouse IgG2a 4F10 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SMAD5 antibody

SMAD5 monoclonal antibody (M08), clone 5E12 Western Blot analysis of SMAD5 expression in HeLa ( Cat # L013V1 ). Mouse IgG1 5E12 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SMAD5 antibody

SMAD5 monoclonal antibody (M11), clone 1C1 Western Blot analysis of SMAD5 expression in C32 ( Cat # L002V1 ). Mouse IgG2a 1C1 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SMAD5 antibody

r-SMAD5; . Lanes: Lane 1: 5ug of transfected 293T lysate (SMAD1) . Lane 1: 5ug of transfected 293T lysate (SMAD2) . Lane 1: 5ug of transfected 293T lysate (SMAD3) . Lane 1: 5ug of transfected 293T lysate (SMAD4) . Lane 1: 5ug of transfected 293T lysate (SMAD5) . Lane 1: 5ug of transfected 293T lysate (SMAD6) . Lane 1: 5ug of transfected 293T lysate (SMAD7) . Lane 1: 5ug of transfected 293T lysate (SMAD8) . Lane 1: 5ug of transfected 293T lysate (GFP). Primary Antibody Dilution: 1:1000. Secondary Antibody: Goat anti-Rabbit IgG HRP Conjugated. Secondary Antibody Dilution: 1:10,000. Gene Name: SMAD5. Submitted by: Amanda Urick, Medical College of Wisconsin.; SMAD5 antibody - middle region (ARP32006_P050) in r-SMAD5 cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

SMAD5 antibody

Human HepG2; WB Suggested Anti-SMAD5 Antibody. . Titration: 2.5 ug/ml. . Positive Control: HepG2 Whole Cell; SMAD5 antibody - middle region (ARP32006_T100) in Human HepG2 cells using Western Blot Rabbit Aff - Purified Can, Hu, Ms, Rt WB
0.1 mg / €310.00
  AVIVA Systems Biology

SMAD5 antibody

SMAD5 Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €290.00
  Boster Immunoleader

SMAD5 antibody

Western blot analysis of SMAD5 expression in HeLa (A); mouse testis (B); PC12 (C) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt, Sh WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

SMAD5 antibody

Western blot analysis of SMAD5 expression in HeLa (A); mouse testis (B); PC12 (C) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt, Sh WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

SMAD5 antibody

Western blot analysis of SMAD5 expression in HeLa (A); mouse testis (B); PC12 (C) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt, Sh WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

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