SMAD5 antibody

Principal name

SMAD5 antibody

Alternative names for SMAD5 antibody

SMAD family member 5, SMAD 5, SMAD-5, MADH5, MAD homolog 5, JV5-1, Mothers against decapentaplegic homolog 5, Mothers against DPP homolog 5

SwissProt ID

P97454 (Mouse), Q56I99 (Chick), Q99717 (Human), Q9R1V3 (Rat), Q9W7E7 (Danre)

Gene ID

4090 (SMAD5), 17129 (Smad5), 59328

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Bov (Bovine), Chk (Chicken), Ze (Zebrafish), African clawed frog, Sh (Sheep)

Available hosts

Rabbit, Mouse, Goat

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), ELISA (detection) (E(detection)), Flow Cytometry (F), Paraffin Sections (P)

Background of SMAD5 antibody

Bone morphogenetic proteins (BMPs) constitute a large family of sigling molecules that regulate a wide range of critical processes including morphogenesis, cell-fate determition, proliferation, differentiation, and apoptosis (1,2). BMP receptors are members of the TGF-β family of Ser/Thr kise receptors. Ligand binding induces multimerization, autophosphorylation, and activation of these receptors (3-5). They subsequently phosphorylate Smad1 at Ser463 and Ser465 in the carboxy-termil motif SSXS, as well as Smad5 and Smad8 at their corresponding sites. These phosphorylated Smads dimerize with the coactivating Smad4 and translocate to the nucleus, where they stimulate transcription of target genes (5).MAP kises and CDKs 8 and 9 phosphorylate residues in the linker region of Smad1, including Ser206. The phosphorylation of Ser206 recruits Smurf1 to the linker region and leads to the degradation of Smad1 (6). Phosphorylation of this site also promotes Smad1 transcriptiol action by recruiting YAP to the linker region (7).

General readings

Langenfeld,E.M., et al., (2006) Oncogene 25 (5), 685-692

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SMAD5 antibody

Immunohistochemical analysis of paraffin-embedded brain tissues (left) and lung cancer tissues (right) using SMAD5 antibody Cat.-No AM06562SU-N with DAB staining. Mouse IgG1 3H9 Ascites Hu, Rt E, F, ICC/IF, P, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

SMAD5 antibody

  Anti-SMAD5 Rabbit Polyclonal Antibody  
Predicted band size: 52 kDa . Positive control: Hela cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/250 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 20 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SMAD5 antibody

  Anti-SMAD5 Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm. Positive control: Human breast and lung cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human breast cancer tissue using SMAD5 antibody at dilution 1/20, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

SMAD5 antibody

  Rabbit Polyclonal Anti-SMAD5 Antibody  
WB Suggested Anti-SMAD5 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

SMAD5 antibody

  Rabbit Polyclonal Anti-SMAD5 Antibody  
WB Suggested Anti-SMAD5 Antibody; Titration: 2.5 ug/ml; Positive Control: HepG2 Whole Cell Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SMAD5 antibody

  Rabbit anti-SMAD5 Polyclonal Antibody  
Western blot analysis of extracts of K562 cell lines, using SMAD5 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

SMAD5 antibody

  Mouse Monoclonal SMAD5 (C-terminus) Antibody  
Western blot detection of SMAD5 (C-terminus) in Hela,Jurkat and K562 cell lysates using SMAD5 (C-terminus) mouse mAb (1:1000 diluted).Predicted band size:52KDa.Observed band size:60KDa. Mouse IgG1 4B10-B10-B6 Purified F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SMAD5 antibody

SMAD5 Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €350.00
  Acris Antibodies GmbH

SMAD5 (155-258) antibody

SMAD5 staining paraffin-embedded human colon tissue sections (dilution  5 µg/mL). Tissues were stained usiing anti-goat HRP-DAB (brown) and counterstained with hematoxylin (blue). Goat IgG Aff - Purified Hu, Ms, Rt C, E, WB
0.1 mg / €450.00
  Neuromics Antibodies

CRISPR

Catalog No. Species Donor Vector  

SMAD5 - human gene knockout kit via CRISPR

SMAD5 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

SMAD5 - human gene knockout kit via CRISPR

SMAD5 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

SMAD5 - human gene knockout kit via CRISPR

SMAD5 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

SMAD5 - human gene knockout kit via CRISPR

SMAD5 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Smad5 - mouse gene knockout kit via CRISPR

Smad5 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Smad5 - mouse gene knockout kit via CRISPR

Smad5 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Smad5 - mouse gene knockout kit via CRISPR

Smad5 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Smad5 - mouse gene knockout kit via CRISPR

Smad5 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

SMAD5 (full length, N-term HIS tag, transcript variant 1)

  Purified recombinant protein of Human SMAD family member 5 (SMAD5), transcript variant 1, full length, with N-terminal HIS tag, expressed in E. coli, 50ug  
SMAD5 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Smad5 (GFP-tagged) - Mouse MAD homolog 5 (Drosophila) (Smad5), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, Smad5 (GFP-tagged) - Mouse MAD homolog 5 (Drosophila) (Smad5), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €1,070.00
  OriGene Technologies, Inc.

Lenti ORF particles, Smad5 (GFP-tagged) - Mouse MAD homolog 5 (Drosophila) (Smad5), transcript variant 3, 200 uL, >10^7 TU/mL

Lenti ORF particles, Smad5 (GFP-tagged) - Mouse MAD homolog 5 (Drosophila) (Smad5), transcript variant 3, 200 uL, >10^7 TU/mL  
200 µl / €1,170.00
  OriGene Technologies, Inc.

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