SMAD7 antibody

Principal name

SMAD7 antibody

Alternative names for SMAD7 antibody

SMAD family member 7, SMAD-7, SMAD 7, MADH7, MAD homolog 7, MADH8, MAD homolog 8, Mothers against decapentaplegic homolog 7, Mothers against DPP homolog 7

SwissProt ID

O15105 (Human), O35253 (Mouse), O88406 (Rat)

Gene ID

4092 (SMAD7), 17131 (Smad7), 81516

Available reactivities

Hu (Human), Can (Canine), Ms (Mouse), Rt (Rat), Por (Porcine), Sh (Sheep)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of SMAD7 antibody

SMADs are members of the MAD-related family of molecules. MAD-related proteins are a family of intracellular proteins that are essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily (1). SMADs can be divided into receptor-regulated SMADs (R-SMADs: SMAD1, SMAD2, SMAD5, SMAD8 and SMAD9), common-mediator SMAD (co-SMAD: SMAD4), and inhibitory SMADs (I-SMADs: SMAD6 and SMAD7). SMAD1, SMAD5, SMAD8 and SMAD9 have high degrees of homology and antibodies are available that recognize sequences common to all of them. 
Human SMAD3 is a 425 amino acid protein; Human SMAD4 is a 552 amino acid protein; Human SMAD5 is a 465 amino acid protein; Human SMAD6 is a 496 amino acid protein; Human SMAD7 is a 426 amino acid protein; Mouse SMAD8 is a 430 amino acid protein; Human SMAD9 is a 430 amino acid protein (SMAD8 and SMAD9 are typically used as alternate names for one another in the literature.)

General readings

Suh,Y.A., (2006) Biochem. Biophys. Res. Commun. 341 (3), 889-894

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SMAD7 antibody

  Rabbit polyclonal anti-Smad7 antibody  
Western blot analysis of Smad7 expression in Jurkat cell lysate (Lane 1& 2) and 3T3 cell lysate (Lane 3). Rabbit Aff - Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SMAD7 antibody

  Anti-SMAD7 rabbit polyclonal antibody  
Predicted band size: 46 kDa . Positive control: Mouse heart tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/1000 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 40 seconds) Rabbit IgG Purified Hu, Ms, Rt P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SMAD7 antibody

  Anti-SMAD7 Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm,Nucleus. Positive control: Human liver and thyroid cancer tissue. Recommended dilution: 1/50-200  The image on the left is immunohistochemistry of paraffin-embedded human liver cancer tissue using SMAD7 antibody at dilution 1/40, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

SMAD7 antibody

  Rabbit polyclonal Smad7 Antibody (Center)  
Western blot analysis of Smad7 Antibody (Center) (Cat. #TA324865) in ZR-75-1 cell line lysates (35ug/lane). Smad7 (arrow) was detected using the purified Pab. Rabbit IgG Purified Hu, Rt ICC/IF, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

SMAD7 antibody

  Rabbit Polyclonal Anti-SMAD7 Antibody  
WB Suggested Antibody Titration: 0.2-1 ug/ml; Positive Control: Lung Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

SMAD7 antibody

  Rabbit Polyclonal Anti-SMAD7 Antibody  
WB Suggested Anti-SMAD7 Antibody Titration: 1.0 ug/ml; Positive Control: Human Lung Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SMAD7 antibody

  Rabbit Polyclonal Smad7 Antibody  
Western Blot: SMAD7 Antibody [TA337039] - Analysis using SMAD7 antibody. Lysate from HepG2 cells probed with SMAD7 antibody at 5 ug/ml. Rabbit IgG Purified Hu, Ms, Rt, Sh WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SMAD7 antibody

  Rabbit Polyclonal Anti-Smad7 Antibody  
Western blot analysis of Smad7 expression in Human Kidney lysate Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

SMAD7 (12-29; 36-50) antibody

Immunohistochemistry staining of human skeletal muscle (Formalin-fixed, Paraffin-embedded tissue after heat-induced antigen retrieval) using SMAD7 antibody Cat.-No. AP07193PU-N.
  Rabbit IgG Purified Hu, Ms, Rt P, WB
50 µg / €470.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

SMAD7 - human gene knockout kit via CRISPR

SMAD7 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

SMAD7 - human gene knockout kit via CRISPR

SMAD7 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

SMAD7 - human gene knockout kit via CRISPR

SMAD7 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

SMAD7 - human gene knockout kit via CRISPR

SMAD7 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Smad7 - mouse gene knockout kit via CRISPR

Smad7 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Smad7 - mouse gene knockout kit via CRISPR

Smad7 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Smad7 - mouse gene knockout kit via CRISPR

Smad7 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Smad7 - mouse gene knockout kit via CRISPR

Smad7 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

SMAD7

  Recombinant protein of human SMAD family member 7 (SMAD7)  
SMAD7 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

Lysates

Catalog No.    

SMAD7 overexpression lysate

SMAD7 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, SMAD7 (mGFP-tagged) - Human SMAD family member 7 (SMAD7), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, SMAD7 (mGFP-tagged) - Human SMAD family member 7 (SMAD7), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

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