SMAD7 antibody

Principal name

SMAD7 antibody

Alternative names for SMAD7 antibody

SMAD family member 7, SMAD-7, SMAD 7, MADH7, MAD homolog 7, MADH8, MAD homolog 8, Mothers against decapentaplegic homolog 7, Mothers against DPP homolog 7

SwissProt ID

O15105 (Human), O35253 (Mouse), O88406 (Rat)

Gene ID

4092 (SMAD7)

Available reactivities

Hu (Human), Can (Canine), Ms (Mouse), Rt (Rat), Por (Porcine), Sh (Sheep)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of SMAD7 antibody

SMADs are members of the MAD-related family of molecules. MAD-related proteins are a family of intracellular proteins that are essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily (1). SMADs can be divided into receptor-regulated SMADs (R-SMADs: SMAD1, SMAD2, SMAD5, SMAD8 and SMAD9), common-mediator SMAD (co-SMAD: SMAD4), and inhibitory SMADs (I-SMADs: SMAD6 and SMAD7). SMAD1, SMAD5, SMAD8 and SMAD9 have high degrees of homology and antibodies are available that recognize sequences common to all of them. SMAD8 and SMAD9 are typically used as alternate names for one another in the literature. Human SMAD1 is a 465 amino acid protein; GenBank Accession No. AAP36050.1. Human SMAD2 is a 467 amino acid protein; GenBank Accession No. AAC51918.1 Human SMAD3 is a 425 amino acid protein; GenBank Accession No. NP_005893.1 Human SMAD4 is a 552 amino acid protein GenBank Accession No. NP_005350.1. Human SMAD5 is a 465 amino acid protein; GenBank Accession No. AAC50791.1. Human SMAD6 is a 496 amino acid protein; GenBank Accession No. AAH12986.1 Human SMAD7 is a 426 amino acid protein; GenBank Accession No. AAB81354.1 Mouse SMAD8 is a 430 amino acid protein; GenBank Accession No. AAN85445.1 Human SMAD9 is a 430 amino acid protein; GenBank Accession No. NP_005896.1

General readings

Suh,Y.A., (2006) Biochem. Biophys. Res. Commun. 341 (3), 889-894

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SMAD7 antibody

Western blot analysis of Smad7 expression in Jurkat cell lysate (Lane 1& 2) and 3T3 cell lysate (Lane 3). Rabbit Aff - Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SMAD7 antibody

Predicted band size: 46 kDa . Positive control: Mouse heart tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/1000 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 40 seconds) Rabbit IgG Purified Hu, Ms, Rt P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SMAD7 antibody

Predicted cell location: Cytoplasm,Nucleus. Positive control: Human liver and thyroid cancer tissue. Recommended dilution: 1/50-200  The image on the left is immunohistochemistry of paraffin-embedded human liver cancer tissue using SMAD7 antibody at dilution 1/40, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

SMAD7 antibody

Western blot analysis of Smad7 Antibody (Center) (Cat. #TA324865) in ZR-75-1 cell line lysates (35ug/lane). Smad7 (arrow) was detected using the purified Pab. Rabbit IgG Purified Hu, Rt ICC/IF, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

SMAD7 antibody

WB Suggested Antibody Titration: 0.2-1 ug/ml; Positive Control: Lung Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

SMAD7 antibody

WB Suggested Anti-SMAD7 Antibody Titration: 1.0 ug/ml; Positive Control: Human Lung Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SMAD7 antibody

Western Blot: SMAD7 Antibody [TA337039] - Analysis using SMAD7 antibody. Lysate from HepG2 cells probed with SMAD7 antibody at 5 ug/ml. Rabbit IgG Purified Hu, Ms, Rt, Sh WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SMAD7 antibody

Western blot analysis of Smad7 expression in Human Kidney lysate Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

SMAD7 (12-29; 36-50) antibody

Immunohistochemistry staining of human skeletal muscle (Formalin-fixed, Paraffin-embedded tissue after heat-induced antigen retrieval) using SMAD7 antibody Cat.-No. AP07193PU-N.
  Rabbit IgG Purified Hu, Ms, Rt P, WB
50 µg / €470.00
  Acris Antibodies GmbH

SMAD7 antibody

SMAD7 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

SMAD7 antibody

Detection limit for recombinant GST tagged SMAD7 is approximately 0.1ng/ml as a capture antibody. Mouse IgG2a 3G8 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SMAD7 antibody

Detection limit for recombinant GST tagged SMAD7 is approximately 1ng/ml as a capture antibody. Mouse IgG2a 1C4 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SMAD7 antibody

Detection limit for recombinant GST tagged SMAD7 is approximately 0.1ng/ml as a capture antibody. Mouse IgG2a 3E9 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SMAD7 antibody

SMAD7 monoclonal antibody (M05), clone 2G6. Western Blot analysis of SMAD7 expression in U-2 OS ( Cat # L022V1 ). Mouse IgG2a 2G6 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SMAD7 antibody

SMAD7 monoclonal antibody (M06), clone 4E1. Western Blot analysis of SMAD7 expression in human placenta. Mouse IgG2a 4E1 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SMAD7 antibody

SMAD7 monoclonal antibody (M07), clone 4G11. Western Blot analysis of SMAD7 expression in human parotid gland. Mouse IgG2a 4G11 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SMAD7 antibody

Detection limit for recombinant GST tagged SMAD7 is approximately 1ng/ml as a capture antibody. Mouse IgG2a 2G2 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SMAD7 antibody

Immunoperoxidase of monoclonal antibody to SMAD7 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml] Mouse IgG2a 1G10 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SMAD7 antibody

SMAD7 Mouse IgG2b 4C11 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SMAD7 antibody

SMAD7 monoclonal antibody (M11), clone 3A12. Western Blot analysis of SMAD7 expression in human placenta. Mouse IgG2a 3A12 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

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