SMARCA2 / BRM antibody

Principal name

SMARCA2 / BRM antibody

Alternative names for SMARCA2 / BRM antibody

SNF2L2, SNF2A, SNF2-alpha, BAF190B, ATP-dependent helicase SMARCA2, Probable global transcription activator SNF2L2, Protein brahma homolog, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 2, BRG1-associated factor 190B

SwissProt ID

P51531 (Human)

Gene ID

6595 (SMARCA2)

Available reactivities

Bov (Bovine), Can (Canine), Chk (Chicken), Hu (Human), Ms (Mouse), Rt (Rat), Ze (Zebrafish), African clawed frog, Fi (Fish), Xen (Xenopus), Mky (Monkey)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of SMARCA2 / BRM antibody

Chromatin, the physiological packaging structure of histone proteins and DNA, is considered a key element in regulating gene expression. Several complexes involved in transcriptional regulation function by either modifying histones or altering chromatin structure. Postranslational modifications of histones, such as acetylation, phosphorylation and methylation, contribute to the regulation of transcription. The ATP-dependent chromatin-remodeling complexes alter chromatin structure by using the energy of ATP hydrolysis to locally disrupt the association of histones with DNA, displacing the nucleosomes from promoter and enhancer regions, and therefore allowing transcription initiation. Chromatin remodeling complexes have been purified from a variety of organisms, and most cell types contain more than one type of complex. These complexes contain structurally related catalytic subunits, but differ in the way in which they manipulate chromatin. Three families of complexes have been described the SWI/SNF family, ISWI family, and Mi-2 family. The SWI/SNF family of ATP-dependent remodeling complexes was identified in yeast, drosophila, and human. It causes nucleosomes to change structure and/or position in order to allow transcriptional activators to gain access to their target sites. In humans, two conserved ATPase subunits have been identified as hBrm (also designated hSNF2alpha) and Brg1 (also designated as SNF2beta). Brg1 (1,613 amino acids) and hBrm (1,586 amino acids) share approximately 52% identity. Components of the hSWI/SNF complexes have been implicated in a range of cellular events including gene activation, regulation of cell growth, and development. Brg1 and hBrm enhances transcriptional activation by glucocorticoid receptors. Apparently, Brg1 and Brm complexes direct distinct cellular processes by recruitment to specific promoters through protein-protein interactions that are unique to each ATPase. The remodeling complexes were traditionally associated with transcriptional activation. However, SWI/SNF has been found associated with repressor complexes, such as HDAC (histone deacetylase) and Rb (retinoblastoma) in a complex that leads to cell cycle arrest, suggesting that they are associated with transcriptional repression. Antibodies reacting specifically with hBrm/SNF2alpha may be used for studying the effects of chromatin remodeling on gene expression.

General readings

Muchardt,C., et al., (1994) Mamm. Genome 5 (4), 241-242

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SMARCA2 / BRM antibody

SMARCA2 / BRM Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

SMARCA2 / BRM antibody

SMARCA2 / BRM Mouse Hu WB
0.5 mg / €1,400.00
  Abnova Taiwan Corp.

SMARCA2 / BRM antibody

Detection limit for recombinant GST tagged SMARCA2 is approximately 0.1ng/ml as a capture antibody. Mouse IgG2a 2D12 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SMARCA2 / BRM antibody

Western blot analysis of extract from MDA-MB-435 cells ( lane 1 ) , SW-13 cells ( lane 2 ) , vector transgenic cell SW-13 cells ( lane3 ) , Brm transgenic cell SW-13 cells ( lane 4 ) and BRGI transgenic cell SW-13 cells ( lane 5 ) , using SMARCA2 polyclonal antibody ( Cat # PAB8486 ) . Rabbit Hu IP, WB
25 µg / €660.00
  Abnova Taiwan Corp.

SMARCA2 / BRM antibody

Human Skin; SMARCA2 antibody - middle region (ARP34484_P050) in Human Skin cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Rt, Ze P, WB
50 µg / €350.00
  AVIVA Systems Biology

SMARCA2 / BRM antibody

Western blot analysis of BRM expression in HepG2 (A); HeLa (B); THP1 (C); NIH3T3 (D) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Mky, Ms, Rt WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

SMARCA2 / BRM antibody

Western blot analysis of BRM expression in HepG2 (A); HeLa (B); THP1 (C); NIH3T3 (D) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Mky, Ms, Rt WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

SMARCA2 / BRM antibody

Western blot analysis of BRM expression in HepG2 (A); HeLa (B); THP1 (C); NIH3T3 (D) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Mky, Ms, Rt WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

SMARCA2 / BRM antibody

SMARCA2 / BRM Rabbit Aff - Purified Hu IP, WB
25 µg / €630.00
  Funakoshi Co., Ltd.

SMARCA2 / BRM antibody

WB and Immunoprecipitation: SMARCA2/BRM Antibody [NB100-55307] - Detection of Human SMARCA2/BRM on HeLa whole cell lysate using NB100-55307.  SMARCA2/BRM was also IPed by rabbit anti-SMARCA2/BRM antibodies NB100-55308 and NB100-55309. Rabbit Aff - Purified Hu IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

SMARCA2 / BRM antibody

WB and Immunoprecipitation: SMARCA2/BRM Antibody [NB100-55308] - Detection of Human SMARCA2/BRM on HeLa whole cell lysate using NB100-55307.  SMARCA2/BRM was also IPed by rabbit anti-SMARCA2/BRM antibodies NB100-55308 and NB100-55309. Rabbit Aff - Purified Hu IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

SMARCA2 / BRM antibody

WB and Immunoprecipitation: SMARCA2/BRM Antibody [NB100-55309] - Detection of Human SMARCA2/BRM on HeLa whole cell lysate using NB100-55307.  SMARCA2/BRM was also IPed by rabbit anti-SMARCA2/BRM antibodies NB100-55308 and NB100-55309. Rabbit Aff - Purified Hu IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

SMARCA2 / BRM antibody

SMARCA2 / BRM Rabbit Aff - Purified Bov, Can, Chk, Hu, Ms, Rt, Xen, Ze P, WB
50 µg / €440.00
  Novus Biologicals Inc.

SMARCA2 / BRM antibody

Immunohistochemistry-Paraffin: BRM Antibody [NBP1-90015] - Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

SMARCA2 / BRM

SMARCA2 / BRM Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

SMARCA2 / BRM

SMARCA2 / BRM Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

16 Item(s)

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