SMARCA4 / BRG1 antibody

Principal name

SMARCA4 / BRG1 antibody

Alternative names for SMARCA4 / BRG1 antibody

SNF2B, SNF2L4, SNF2-beta, BRG-1 protein, Brahma protein homolog 1, Probable global transcription activator SNF2L4, ATP-dependent helicase SMARCA4, Mitotic growth and transcription activator, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4

SwissProt ID

P51532 (Human)

Gene ID

6597 (SMARCA4)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine)

Available hosts

Mouse, Rabbit, Goat, Rat

Available applications

Enzyme Immunoassay (E), Paraffin Sections (P), Immunoprecipitation (IP), PLA/Proximity ligation assay (PLA), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C)

Background of SMARCA4 / BRG1 antibody

The modulation of chromatin structure is an essential component in the regulation of transcriptiol activation and repression. Modifications can be made by at least two evolutiorily conserved strategies, through the disruption of histone-D contacts by ATP-dependent chromatin remodelers, or by histone tail modifications including methylation and acetylation. One of the four classes of ATP-dependent histone remodelers is the SWI/SNF complex, the central catalytic subunit of which is Brg1 or the highly related protein hBRM . This SWI/SNF complex contains varying subunits but its association with either Brg1 or hBRM remains constant . SWI/SNF complexes have been shown to regulate gene activation, cell growth, the cell cycle and differentiation . Brg1/hBRM have been shown to regulate transcription through enhancing transcriptiol activation of glucocorticoid receptors . Although usually associated with transcriptiol activation, Brg1/hBRM have also been found in complexes associated with transcriptiol repression including with HDACs, Rb and Tif1ß . Brg1/hBRM plays a vital role in the regulation of gene transcription during early mammalian embryogenesis. In addition, Brg1/hBRM also play a role as a tumor suppressors and Brg1 is mutated in several tumor cell lines.

18 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SMARCA4 / BRG1 antibody

Predicted band size: 185 kDa . Positive control: K562, 293T cell and hepG2 lysate. Recommended dilution: 1/200-1000. (Gel: 8%SDS-PAGE Lane1: K562, lysate Lane2: 293T cell lysate Lane3: HepG2 cell lysate Lysates: 50 ug per lane Primary antibody: 1/300 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SMARCA4 / BRG1 antibody

Predicted band size: 185 kDa . Positive control: Hela and Jurkat cell lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lane1: Hela cell lysate Lane2: Jurkat cell lysate Lysates: 50 ug per lane Primary antibody: 1/700 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 30 seconds) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SMARCA4 / BRG1 antibody

Western blot analysis of extracts of various cell lines, using SMARCA4 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

SMARCA4 / BRG1 antibody

Western blot detection of SBRG1 in Hela NE and K562 cell lysates using BRG1 mouse mAb (1:1000 diluted).Predicted band size:220KDa.Observed band size:220KDa. Mouse IgG1 4E11-E11-G11 Purified WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SMARCA4 / BRG1 antibody

Western blot detection of BRG1 in Hela NE,F9 and K562 cell lysates using BRG1 mouse mAb (1:1000 diluted).Predicted band size:220KDa.Observed band size:220KDa. Mouse IgG1 6D7-F7-B6 Purified ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SMARCA4 / BRG1 antibody

Western blot analysis of extracts from 293 and HepG2 cells using Brg1 Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SMARCA4 / BRG1 antibody

Western blot analysis of SMARCA4 in SK-N-SH cell lysate with SMARCA4 antibody at 1 ug/mL. Rabbit IgG Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SMARCA4 / BRG1 (C-term) antibody

Immunostaining of Brg1 by BRG-01 antibody. 
A - Brg1-negative SW13 cells were transfected with Brg1-expression plasmid and analysed by Western blot for Brg1 expression. 
B - HEK293 cytoplasmic (cyt) and nuclear (nuc) lysates were analysed by Western blot for Brg1 presence. Mouse IgG2a BRG-01 Aff - Purified Hu P, WB
0.1 mg / €260.00
  Acris Antibodies GmbH

SMARCA4 / BRG1 (2-14) antibody

Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE) Goat IgG Aff - Purified Bear, Bov, Bt, Can, Chk, Elephant, Eq, Fi, Hst, Hu, Mky, Op, Por, Prim, Rb, Rt, Xen P
50 µg / €470.00
  Acris Antibodies GmbH

SMARCA4 / BRG1 antibody

SMARCA4 / BRG1 Mouse Serum Hu E
50 µl / €290.00
  Abnova Taiwan Corp.

SMARCA4 / BRG1 antibody

SMARCA4 / BRG1 Rabbit Hu, Ms IP, WB
0.1 ml / €470.00
  Abnova Taiwan Corp.

SMARCA4 / BRG1 antibody

Brg1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human Brg1 Antigen Affinity-purified Polyclonal Antibody at 10 μg/mL for 3 hours at room temperature. Cells were stained with 557-conjugated anti-Goat IgG Secondary Antibody (red, upper panel) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. Goat IgG Aff - Purified Hu, Ms ICC/IF, WB
0.1 mg / €450.00
  Neuromics Antibodies

SMARCA4 / BRG1 antibody

Immunohistochemistry-Paraffin: BRG1 Antibody [NB100-2594] - Please see notes section Rabbit Aff - Purified Hu, Ms IP, P, PLA, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

SMARCA4 / BRG1 antibody

SMARCA4 / BRG1 Goat Hu, Ms IP
0.1 mg / €500.00
  Novus Biologicals Inc.

SMARCA4 / BRG1 antibody

Western Blot: BRG1 Antibody (EPR3913) [NBP1-40785] - Western blot analysis on A) K562 and B) Molt-4 cell lysates using anti-BRG1 Rabbit IgG EPR3913 Hu, Ms WB
0.1 ml / €420.00
  Novus Biologicals Inc.

SMARCA4 / BRG1 antibody

SMARCA4 / BRG1 Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €420.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

SMARCA4 / BRG1

SMARCA4 / BRG1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

SMARCA4 / BRG1

SMARCA4 / BRG1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

18 Item(s)

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