Hu (Human), Can (Canine), Ms (Mouse), Rt (Rat), Bov (Bovine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of SMARCC1 / BAF155 antibody
SMARCC1 is involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). It may stimulate the ATPase activity of the catalytic subunit of the complex. It is a component of five multiprotein chromatin remodeling complexes: Swi/Snf-A (BAF), Swi/Snf-B (PBAF), Brm, Brg1(I) and Brg1(II). Each of the five complexes contains a catalytic subunit (either SMARCA4 or SMARCA2), and at least SMARCE1, ACTL6A/BAF53A or ACTL6B/BAF53B, SMARCC2 and SMARCB1. Other subunits specific to each of the complexes may also be present. It may also interact with the SIN3A histone deacetylase transcription repressor complex in conjunction with SMARCA2 and SMARCA4. The minimal complex composed of SMARCC1 and SMARCA4 seems to be able to associate with cyclin such as CCNE1 or transcription factors such as KLF1 or GATA1.
Phelan,M.L., et al., (1999) Mol. Cell 3 (2), 247-253
SMARCC1 polyclonal antibody (A01), Lot # 051012JC01 Western Blot analysis of SMARCC1 expression in SJCRH30 ( Cat # L027V1 ).
Human Jurkat; WB Suggested Anti-SMARCC1 Antibody Titration: 1.25ug/ml. ELISA Titer: 1:312500. Positive Control: Jurkat cell lysate. SMARCC1 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells.; SMARCC1 antibody - C-terminal region (ARP33307_T100) in Human Jurkat cells using Western Blot
Western Blot and IP: SMARCC1/BAF155 Antibody [NB100-55312] - Detection of Human SMARCC1/BAF155 on HeLa whole cell lysate using NB100-55312. SMARCC1/BAF155 was also IPed by rabbit anti-SMARCC1/BAF155 antibodies NB100-55313 and NB100-55314.
Western Blot and IP: SMARCC1/BAF155 Antibody [NB100-55314] - Detection of Human SMARCC1/BAF155 on HeLa whole cell lysate using NB100-55314. SMARCC1/BAF155 was also IPed by rabbit anti-SMARCC1/BAF155 antibodies NB100-55312 and NB100-55313.
SMARCC1 antibody [C2C3], C-term detects SMARCC1 protein at nucleus on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. SMARCC1 antibody [C2C3], C-term (GTX114777) dilution: 1:500.
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using SMARCC1(GTX114777) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
SMARCC1 antibody immunoprecipitates SMARCC1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 30 μg whole cell lysate/extract of SMARCC1 protein expressing 293T cells B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of SMARCC1 by 2.5 μg of SMARCC1 antibody (GTX114777) 5% SDS-PAGE The immunoprecipitated SMARCC1 protein was detected by SMARCC1 antibody (GTX114777) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.
Cross-linked ChIP was performed with MF-7 chromatin extract treated with B-estradiol (10 nM for 45 min) and 5 μg of either control rabbit IgG or anti-SMARCC1 antibody. The precipitated DNA was detected by PCR with primer set targeting to ESR1 or Sat2.
Human Jurkat; WB Suggested Anti-SMARCC1 Antibody Titration: 1.25ug/ml. ELISA Titer: 1:312500. Positive Control: Jurkat cell lysate. SMARCC1 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells.; SMARCC1 antibody - C-terminal region (AP42196PU-N) in Human Jurkat cells using Western Blot