SMO antibody

Principal name

SMO antibody

Alternative names for SMO antibody

SMOH, Smoothened homolog, Gx protein

SwissProt ID

O42224 (Chick), P56726 (Mouse), P91682 (Drome), P97698 (Rat), Q99835 (Human)

Gene ID

6608 (SMO)

Available reactivities

Bt (Bat), Bov (Bovine), Can (Canine), Chk (Chicken), Eq (Equine), Hst (Hamster), Hu (Human), Mky (Monkey), Ms (Mouse), Op (Opossum), Por (Porcine), Rb (Rabbit), Rt (Rat), Xen (Xenopus), Bear, Ze (Zebrafish), Prim (Primate)

Available hosts

Rabbit, Mouse, Goat

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Enzyme Immunoassay (E), Western blot / Immunoblot (WB)

Background of SMO antibody

Overexpression of either Wnt-1 or the GLI proteins results in cancer; however, the molecular basis for this transformation was poorly understood. The Wnt-1 and GLI proteins have now been placed in a signaling cascade downstream of the mammalian homologs of the Drosophila hedgehog and patched proteins. The Drosophila segment polarity gene hedgehog (hh) encodes a 19-25 kDa secreted protein that appears to function in embryonic and imaginal disc patterning. The ptc gene, also identified as a Drosophila segment polarity gene, encodes the transmembrane protein patched, the expression of which is precisely regulated during embryonic development. Hedgehog has been shown to enhance the expression of the Wnt family of proteins through a signaling cascade involving the GLI transcription factors, while patched functions as a repessor opposing hedgehog’s effects. Smoothened (Smo), a seven transmembrane receptor, is complexed with patched in many tissues and is believed to be an essential component in the Hh signaling pathway.

General readings

Taipale J, Cooper MK, Maiti T, Beachy PA.
Patched acts catalytically to suppress the activity of Smoothened.
Nature. 2002 Aug 22;418(6900):892-7. Erratum in: Nature 2002 Nov
28;420(6914):445.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SMO (N-term, extracell. dom.) antibody

Human Kidney: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Bear, Bov, Bt, Can, Chk, Elephant, Eq, Hst, Hu, Mky, Ms, Op, Platypus, Por, Prim, Rb, Rt, Tr P, WB
50 µg / €379.00
  OriGene Technologies, Inc.

SMO (1st Extracell. Dom.) antibody

Human, Testis: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Bear, Bov, Bt, Can, Chk, Elephant, Eq, Hst, Hu, Mky, Ms, Op, Por, Rb, Rt, Xen ICC/IF, P
50 µg / €379.00
  OriGene Technologies, Inc.

SMO (C-term) antibody

A) TA303075 and B) TA303149 (0.3µg/ml) staining of Human Bone Marrow lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence Goat Aff - Purified Bov, Can, Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SMO antibody

A) TA303075 and B) TA303149 (0.3µg/ml) staining of Human Bone Marrow lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt, Ze E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SMO antibody

Western blot analysis of extracts from HuvEc cells, using SMO antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SMO antibody

Anti-SMO antibody IHC of human placenta, cytotrophoblast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. Mouse IgG2a 3E5 Purified Hu E, P, WB
50 µg / €345.00
  OriGene Technologies, Inc.

SMO antibody

Predicted cell location: Cytoplasm. Positive control: Human brain and thyroid cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human brain tissue using SMO antibody at dilution 1/20, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

SMO antibody

Western Blot: Smoothened Antibody [TA337000] - Analysis in human heart lysate in the 1) absence and 2) presence of immunizing peptide using SMO antibody at 0.1 ug/ml. Rabbit Purified Hu, Ms, Prim P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SMO antibody

Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2a 3D10 Purified Hu E, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

SMO antibody

Western blot (WB) analysis of Smo antibody (Cat.-No.: AP01508PU-N) in extracts from HUVEC cells. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €350.00
  Acris Antibodies GmbH

SMO Control Peptide

SMO IgG Purified
50 µg / €210.00
  Acris Antibodies GmbH

SMO antibody

Western blot using SMO antibody on human brain lysate. Lysate loaded at 15 µg/lane. Antibody used at 10 µg/ml (1) and 5 µg/ml (2) dilution. Secondary antibody, mouse anti-rabbit-HRP, used at 1:150K dilution. Rabbit IgG Aff - Purified Hu WB
10 Blots / €480.00
  Acris Antibodies GmbH

SMO antibody

Western blot using SMO antibody (AP05293SU-N) on human brain lysate. Lysate loaded at 15 µg/lane. Antibody used at 10 µg/ml (1) and 5 µg/ml (2) dilution. Secondary antibody, mouse anti-rabbit-HRP, used at 1:150K dilution. Rabbit IgG Purified Hu WB
0.1 mg / €370.00
  Acris Antibodies GmbH

SMO (N-term) antibody

Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Hu, Ms, Rt E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

SMO (Center) antibody

SMO Antibody (Center) (Cat. #AP53947PU-N) western blot analysis in HepG2 cell line lysates (35µg/lane).This demonstrates the SMO antibody detected the SMO protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

SMO antibody

SMO Mouse Serum Hu E
50 µl / €290.00
  Abnova Taiwan Corp.

SMO antibody

SMO Mouse IgG1 1D6 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

SMO antibody

SMO Mouse IgG1 1G11 Ascites Hu E
0.2 ml / €370.00
  Abnova Taiwan Corp.

SMO antibody

Detection limit for recombinant GST tagged SMO is approximately 0.3ng/ml as a capture antibody. Mouse IgG1 3G8 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

SMO antibody

Detection limit for recombinant GST tagged SMO is approximately 0.3ng/ml as a capture antibody. Mouse IgG1 2F8 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

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