SMPD1 antibody

Principal name

SMPD1 antibody

Alternative names for SMPD1 antibody

Sphingomyelin phosphodiesterase, Acid sphingomyelinase, ASM

SwissProt ID

P17405 (Human), Q04519 (Mouse), Q0VD19 (Bovin)

Gene ID

6609 (SMPD1), 20597 (Smpd1)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Rt (Rat), Sh (Sheep), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P)

Background of SMPD1 antibody

Kit Component:
- KN316344G1, Smpd1 gRNA vector 1 in pCas-Guide vector
- KN316344G2, Smpd1 gRNA vector 2 in pCas-Guide vector
- KN316344D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

Zeidan,Y.H., (2008) J. Cell Biol. 181 (2), 335-350

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SMPD1 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-SMPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803659) Mouse IgG1 OTI3H7 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SMPD1 antibody

WB Suggested Anti-SMPD1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Human heart Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Sh WB
50 µg / €325.00
  OriGene Technologies, Inc.

SMPD1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SMPD1 (RC219758, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SMPD1. Mouse IgG1 OTI10C5 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SMPD1 antibody

SMPD1 Mouse IgG2a 4H2 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SMPD1 antibody

SMPD1 Rabbit Hu E, WB
0.1 mg / €560.00
  Abnova Taiwan Corp.

SMPD1 antibody

Human Heart; WB Suggested Anti-SMPD1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human heart; SMPD1 antibody - middle region (ARP35630_P050) in Human Heart cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

SMPD1 antibody

Western blot analysis of Acid Sphingomyelinase expression in A431 (A); HepG2 (B); SP2/0 (C); H9C2 (D) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Ms, Rt, Sh WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

SMPD1 antibody

Western blot analysis of Acid Sphingomyelinase expression in A431 (A); HepG2 (B); SP2/0 (C); H9C2 (D) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Ms, Rt, Sh WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

SMPD1 antibody

Western blot analysis of Acid Sphingomyelinase expression in A431 (A); HepG2 (B); SP2/0 (C); H9C2 (D) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Ms, Rt, Sh WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

CRISPR

Catalog No. Species Donor Vector  

SMPD1 - human gene knockout kit via CRISPR

SMPD1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

SMPD1 - human gene knockout kit via CRISPR

SMPD1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

SMPD1 - human gene knockout kit via CRISPR

SMPD1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

SMPD1 - human gene knockout kit via CRISPR

SMPD1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Smpd1 - mouse gene knockout kit via CRISPR

Smpd1 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Smpd1 - mouse gene knockout kit via CRISPR

Smpd1 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Smpd1 - mouse gene knockout kit via CRISPR

Smpd1 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Smpd1 - mouse gene knockout kit via CRISPR

Smpd1 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

SMPD1 (transcript variant 1)

SMPD1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

SMPD1

SMPD1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

SMPD1

SMPD1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

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