SMT3 antibody

Principal name

SMT3 antibody

Alternative names for SMT3 antibody

SMT-3, Ubiquitin-like protein SMT3

SwissProt ID

O97102 (Drome), Q12306 (Yeast)

Gene ID

33981, 852122 (SMT3)

Available reactivities

Dros (Drosophila), Hu (Human), Ye (Yeast), S. cerevisiae (Saccharomyces cerevisiae)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Frozen Sections (C), Enzyme Immunoassay (E)

Background of SMT3 antibody

Covalent modification of cellular proteins by the ubiquitin-like modifier SUMO (small ubiquitin-like modifier) regulates various cellular processes, such as nuclear transport, signal transduction, stress responses and cell cycle progression. However, in contrast to ubiquination, sumoylation does not tag proteins for degradation by the 26S proteasome but rather seems to enhance stability or modulate their subcellular compartmentalization. Ubiquitin-like proteins fall into two classes: the first class, ubiquitin-like modifiers (UBLs) function as modifiers in a manner analogous to that of ubiquitin. Examples of UBLs are SUMO, Rub1 (also called Nedd8), Apg8 and Apg12. Proteins of the second class, including parkin, RAD23 and DSK2, are designated ubiquitin-domain proteins (UDPs). These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other. In contrast to UBLs, UDPs are not conjugated to other proteins. Once covalently attached to cellular targets, SUMO regulates protein:protein and protein:DNA interactions, as well as localization and stability of the target protein. Sumoylation occurs in most eukaryotic systems, and SUMO is highly conserved from yeast to humans. Where invertebrates have only a single SUMO gene termed SMT3, three members of the SUMO family have been identified in vertebrates: SUMO-1 and the close homologues SUMO-2 and SUMO-3. SUMO has been called SMT3 (yeast), sentrin, PIC1, GMP1 and UBL1. SUMO has been shown to bind and regulate mammalian SP-RINGs (such as Mdm2, PIAS and PML), RanGAP1, RanBP2, p53, p73, HIPK2, TEL, c-Jun, Fas, Daxx, TNFRI, Topo-I, Topo-II, WRN, Sp100, IkappaB-alpha, Androgen receptor (AR), GLUT1/4, Drosophila Ttk69, Dorsal, CaMK, yeast Septins, and viral CMV-IE1/2, EBV-BZLF1, HPV/BPV-E1. These bindings implicate SUMO in the stabilization of the target proteins and/or their localization to subcellular complexes. SUMO has an apparent molecular weight of ~12 kDa, and human SUMO-1 (a 101 amino acid polypeptide) shares 50% sequence identity with SUMO-2 and SUMO-3 and with yeast SMT3. SUMO and ubiquitin only show about 18% homology, but both possess a common three-dimensional structure characterized by a tightly packed globular fold with beta-sheets wrapped around an alpha-helix.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SMT3 antibody

Immunoblot of ySUMO fusion protein. Anti-ySUMO antibody, generated by immunization with recombinant yeast SUMO, was tested by immunoblot against a SUMO-GFP fusion protein (lane 2). While the actual molecular weight of the fusion protein is 39 kDa, the protein migrates as a 49 kDa band (arrowhead). No reactivity is seen for lane 1 which contains His-tagged GFP protein. The membrane was blocked using BLOTTO. Primary antibody was used at a 1:1,000 dilution in BLOTTO. The membrane was washed and reacted with a 1:10,000 dilution of IRDye(R) 800 Conjugated Affinity Purified Goat-anti-Mouset IgG (H&L) MX10 (800 nm channel). Molecular weight estimation was made by comparison to prestained MW markers indicated at the right (lane M, 700 nm channel). Other detection systems will yield similar results. Mouse IgG1 4F2.F5.G2 Purified Ye E, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

SMT3 (C-term) antibody

Figure 1. Western blot analysis using anti-SUMO1 Pab (Cat. #AP11285PU-N) to detect SUMO1 in HL-60 cell lysate. Rabbit Ig Purified Dros, Hu WB

Regular Price: 0.4 ml / €370.00

Special Price: 0.4 ml / €231.00

  Acris Antibodies GmbH

SMT3 antibody

Immunoblot of SMT3 fusion protein.
SMT3 monoclonal antibody, clone 4F2.F5.G2 ( Cat # MAB2093 ), generated by immunization with recombinant yeast SMT3, was tested by immunoblot against a SMT3-GFP fusion protein (lane 2).
While the actual molecular weight of the fusion protein is 39 kDa, the protein migrates as a 49 kDa band ( arrowhead ).
No reactivity is seen for lane 1 which contains His-tagged GFP protein.
The primary antibody was used at a 1:1,000 dilution. Mouse IgG1 4F2.F5.G2 Ye C, E, WB
0.1 mg / €560.00
  Abnova Taiwan Corp.

SMT3 antibody

Immunoblot of SMT3-GFP fusion proteins cleaved by insect cell protein extracts.
SMT3 polyclonal antibody ( Cat # PAB11357 ) , generated by immunization with recombinant yeast SMT3, was tested by immunoblot against several constructs of SMT3-GFP fusion proteins after cleavage by proteases in insect cell protein extracts.
These constructs contained various linkers between the SMT3 and GFP portion of the fusion proteins. Each sample was run twice.
The left lanes each contain 2 µg E.coli expressed and purified SMT3-GFP fusion proteins after incubation with lysed cells ( 50 µgtotal protein ) for 1 h.
The right lanes contain the same fusion proteins incubated with the lysate in the presence of 2% SDS.
After probing with anti-GFP antibodies the membranes were stripped of antibody using SDS-DTT solution for 30 m at 60°C and were then re-probed using the anti-SMT3 antibody at a 1:1000 dilution incubated overnight at 4°C in 5% non-fat drymilk in TTBS.
Detection occurred using a 1:2000 dilution of HRP-labeled Donkey anti-Rabbit IgG for1 hour at room temperature.
A chemiluminescence system was used for signal detection ( Roche ) . Rabbit Ye E, WB
0.5 mg / €560.00
  Abnova Taiwan Corp.

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